Abstract
Biofuel production from lignocellulose feedstocks is sustainable and environmentally friendly. However, the lignocellulosic pretreatment could produce fermentation inhibitors causing multiple stresses and low yield. Therefore, the engineering construction of highly resistant microorganisms is greatly significant. In this study, a composite functional chimeric cellulosome equipped with laccase, versatile peroxidase, and lytic polysaccharide monooxygenase was riveted on the surface of Saccharomyces cerevisiae to construct a novel yeast strain YI/LVP for synergistic lignin degradation and cellulosic ethanol production. The assembly of cellulosome was assayed by immunofluorescence microscopy and flow cytometry. During the whole process of fermentation, the maximum ethanol concentration and cellulose conversion of engineering strain YI/LVP reached 8.68 g/L and 83.41%, respectively. The results proved the availability of artificial chimeric cellulosome containing lignin-degradation enzymes for cellulosic ethanol production. The purpose of the study was to improve the inhibitor tolerance and fermentation performance of S. cerevisiae through the construction and optimization of a synergistic lignin-degrading enzyme system based on cellulosome.
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This work was supported by the National Natural Science Foundation of China under Grant (No. 31971202).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Yutong Ye and Han Liu. The first draft of the manuscript was written by Yutong Ye, and all authors commented on previous versions of the manuscript. All the authors have read and approved the final manuscript.
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Ye, Y., Liu, H., Wang, Z. et al. A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation. Biotechnol Lett (2024). https://doi.org/10.1007/s10529-024-03485-0
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DOI: https://doi.org/10.1007/s10529-024-03485-0