Abstract
Bilirubin, a key active ingredient of bezoars with extensive clinical applications in China, is produced through a chemical process. However, this method suffers from inefficiency and adverse environmental impacts. To address this challenge, we present a novel and efficient approach for bilirubin production via whole-cell transformation. In this study, we employed Corynebacterium glutamicum ATCC13032 to express a β-glucuronidase (StGUS), an enzyme from Staphylococcus sp. RLH1 that effectively hydrolyzes conjugated bilirubin to bilirubin. Following the optimization of the biotransformation conditions, a remarkable conversion rate of 79.7% in the generation of bilirubin was obtained at temperate 40 °C, pH 7.0, 1 mM Mg2+ and 6 mM antioxidant NaHSO3 after 12 h. These findings hold significant potential for establishing an industrially viable platform for large-scale bilirubin production.
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Acknowledgements
This work was supported by the Key Research Program of the Education Department of Anhui Province (grant no. K120438044 to W.Z.) and the Research funding of Anhui University (grant no. S020318003/012 to W.Z.).
Funding
Key Research Program of the Education Department of Anhui Province,K120438044,Wei Zhou,Research funding of Anhui university,S020318003/012,Wei Zhou
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M.Z., Y.Z.W and W.Z. conceived the project. Y.N.C., M.Y.C, Q.J.G., K.B and W.Z. performed experiments. W.Z., M.Z., and Y.N.C. analyzed the data. W.Z. and M.Z. wrote the manuscript.
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Zhou, W., Cui, Y., Chen, M. et al. Production of bilirubin via whole-cell transformation utilizing recombinant Corynebacterium glutamicum expressing a β-glucuronidase from Staphylococcus sp. RLH1. Biotechnol Lett 46, 223–233 (2024). https://doi.org/10.1007/s10529-024-03468-1
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DOI: https://doi.org/10.1007/s10529-024-03468-1