Skip to main content
SpringerLink
Log in

Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objective

To investigate the effect of dihydrotestosterone (DHT) combined with Dezawa’s method on the differentiation of bone marrow stromal cells (BMSCs) into schwann-like cells.

Results

Compared to the Dezawa’s method, schwann-like cells obtained from our modified method were longer and thinner and exhibited a typical bipolar or tripolar shape. These cells had a higher mRNA expression of S100 and myelin protein zero (P0), about 1.7- and 2.5-fold respectively, while the glial fibrillary acidic protein (GFAP) mRNA level was decreased about 92 %. No significant difference in peripheral myelin protein 22 (PMP22) mRNA expression was found. Immunofluorescence and Western blot showed the similar results.

Conclusion

DHT in combination with Dezawa’s method to induce a BMSCs to differentiate into schwann-like cells with higher expression of P0, which might be more effective in clinical application than previous method for nerve regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bronstein JM (2000) Function of tetraspan proteins in the myelin sheath. Curr Opin Neurobiol 10:552–557

    Article  CAS  PubMed  Google Scholar 

  • Desarnaud F, Do TA, Brown AM et al (1998) Progesterone stimulates the activity of the promoters of peripheral myelin protein-22 and protein zero genes in schwann cell. J Neurochem 71:1765–1768

    Article  CAS  PubMed  Google Scholar 

  • Dezawa M, Takahashi I, Esaki M et al (2001) Sciatic nerve regeneration in rats induced by transplantation of in vitro differentiated bone-marrow stromal cells. Eur J Neurosci 14:1771–1776

    Article  CAS  PubMed  Google Scholar 

  • D’Urso D, Ehrhardt P, Muller HW (1999) Peripheral myelin protein 22 and protein zero: a novel association in peripheral nervous system myelin. J Neurosci 19:3396–3403

    PubMed  Google Scholar 

  • Hou SY, Zhang HY, Quan DP et al (2006) Tissue-engineered peripheral nerve grafting by differentiated bone marrow stromal cells. Neuroscience 140:101–110

    Article  CAS  PubMed  Google Scholar 

  • Hussain R, Ghoumari AM, Bielecki B et al (2013) The neural androgen receptor: a therapeutic target for myelin repair in chronic demyelination. Brain 136:132–146

    Article  PubMed  PubMed Central  Google Scholar 

  • Kaewkhaw R, Scutt AM, Haycock JW (2011) Anatomical site influences the differentiation of adipose-derived stem cells for Schwann-cell phenotype and function. Glia 59:734–749

    Article  PubMed  Google Scholar 

  • Liu Y, Chen J, Liu W et al (2016) A modified approach to inducing bone marrow stromal cells to differentiate into cells with mature schwann cell phenotypes. Stem Cell Develop 25:347–359

    Article  CAS  Google Scholar 

  • Magnaghi V, Cavarretta I, Zucchi I et al (1999) Po gene expression is modulated by androgens in the sciatic nerve of adult male rats. Brain Res Mol Brain Res 70:36–44

    Article  CAS  PubMed  Google Scholar 

  • Mantovani C, Mahay D, Kingham M et al (2010) Bone marrow- and adipose-derived stem cells show expression of myelin mRNAs and proteins. Regen Med 5:403–410

    Article  CAS  PubMed  Google Scholar 

  • Melcangi RC, Cavarretta IT, Ballabio M et al (2005) Peripheral nerves: a target for the action of neuroactive steroids. Brain Res Brain Res Rev 48:328–338

    Article  CAS  PubMed  Google Scholar 

  • Melcangi RC, Giatti S, Pesaresi M et al (2011) Role of neuroactive steroids in the peripheral nervous system. Front Endocrinol 2:104

    Article  Google Scholar 

  • Movaghar B, Tiraihi T, Mesbah-Namin SA (2008) Transdifferentiation of bone marrow stromal cells into Schwann cell phenotype using progesterone as inducer. Brain Res 1208:17–24

    Article  CAS  PubMed  Google Scholar 

  • Park HW, Lim MJ, Jung H et al (2010) Human mesenchymal stem cell-derived Schwann cell-like cells exhibit neurotrophic effects, via distinct growth factor production, in a model of spinal cord injury. Glia 58:1118–1132

    Article  PubMed  Google Scholar 

  • Wiberg M, Terenghi G (2003) Will it be possible to produce peripheral nerves? Surg Technol Int 11:303–310

    PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Number: 81471270).

Supporting information

Supplementary Table 1—Sequences of qRT-PCR primers.

Author information

Authors and Affiliations

  1. Department of Hand Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Xiaofan Yang, Jianghai Chen, Ranran Liu, Wei Ji, Xiaocheng Lu & Zhenbing Chen

  2. Department of Pharmacy, Tongji Hospital, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan, 430030, China

    Pingping Xue

  3. Department of Anesthesiology, The People’s Hospital of Hanchuan Renmin Hospital of Wuhan University, Wuhan, 432300, Hubei Province, China

    Xin Liu

Authors
  1. Xiaofan Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jianghai Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pingping Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ranran Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaocheng Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zhenbing Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenbing Chen.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1. Sequences of qRT-PCR primers. (DOC 32 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, X., Chen, J., Xue, P. et al. Differentiation of bone marrow stromal cells into schwann-like cells using dihydrotestosterone combined with a classical induction method. Biotechnol Lett 39, 331–337 (2017). https://doi.org/10.1007/s10529-016-2239-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-016-2239-4

Keywords

Search

Navigation