Tnao38, high five and Sf9—evaluation of host–virus interactions in three different insect cell lines: baculovirus production and recombinant protein expression

Purpose of work: A comparative analysis of new and established insect cell lines, in regard to process relevant parameters, provide data that can be exploited for designing more robust and effective protein production processes. The baculovirus-insect cell expression system has been efficiently used for the production of heterologous proteins. Three different insect cell lines Tnao38, High Five and Sf9 were compared in terms of virus susceptibility, baculovirus production and product yield of an intra-cellularly (YFP) and extra-cellularly (influenza A virus hemagglutinin)-expressed recombinant protein. The Tnao38 and High Five cell lines exhibited higher (tenfold) susceptibility to baculovirus infection than Sf9 cells, whereas Sf9 cells showed a higher (100-fold) capacity for production of infectious virus particles. Analysis of recombinant protein expression revealed considerably higher product yields in Tnao38 and High Five cells as compared to Sf9 cells, for both model proteins. Overall, the two Trichoplusia ni-derived cell lines, High Five and Tnao38, were significantly more efficient in terms of secreting proteins such as the glycoprotein hemagglutinin of influenza A virus.


Introduction
The insect cell-based baculovirus expression vector system (BEVS) is a widely used and safe tool for the production of recombinant proteins and baculovirus particles (Kost and Condreay 1999;Miller 1997). Production is fast and easy and provides high yields, efficient protein secretion and eukaryotic-like glycosylation (Kost et al. 2005). Furthermore, BEVS offers the possibility to co-express several proteins, thereby allowing the production of multi-subunit proteins such as transcription factor complexes or virus-like particles (Berger et al. 2004;Bieniossek et al. 2008;Fernandes et al. 2013;Fitzgerald et al. 2006;Kang et al. 2009;Trowitzsch et al. 2010) which makes it a unique system for many applications in basic research, biotechnology and biopharmacy. The most widely used baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), infects about 40 different Lepidoptera belonging to 11 different families (Doyle et al. 1990). Although, the host range of AcNPV is quite broad and a number of lepidopteran cell lines are established (Lynn 2007), cell lines derived from Spodoptera frugiperda and Trichoplusia ni are mainly used in biotechnology. Spodoptera frugiperda Sf9 cells (Vaughn et al. 1977) are preferentially used for recombinant protein production as well as for baculovirus production. Trichoplusia ni BTI-TN-5B1-4 ''High Five'' cells (Granados et al. 1994;Wickham and Nemerow 1993) have been described to be more efficient in overall yield and especially in terms of secretion, suggested to be advantageous for complex and glycosylated proteins (Bruinzeel et al. 2002;Granados et al. 2007;Krammer et al. 2010). A new Trichoplusia ni cell line BTI-Tnao38 has been established (Hashimoto et al. 2010) and preliminary studies showed that this cell line is feasible for efficient protein secretion and indicated a higher stability during virus infection (Palmberger et al. 2012).
When generated by virus infection, recombinant proteins, their yield and quality, and the robustness of the production process are largely dependent on interactions of host and virus. Influential factors are cell line-specific parameters and genetic features of the virus. Characterization of these interconnections and their comparative analysis are required for setting up standardized, consistent processes that are eligible for generating products, fulfilling quality standards, production requirements and authorities' regulations.
Here we present a comparative analysis of three different insect cell lines (Sf9, High Five, Tnao38) by analysing parameters influenced by host-virus interactions. As the cell lines in our study are of two different species' origin, we sought to determine possible differences in order to expand cell line characterization and identify strategies for improvement of insect cell based production processes. The hypothesis regarding investigation of virus production in different cell lines was that virus produced in a certain cell line may exhibit an increased susceptibility if that virus was titered on that particular cell line. On the other hand, we wanted to validate other insect cell lines, e.g. Tnao38 cell line, in addition to Sf9 cells for baculovirus titration. Besides susceptibility and high titer virus production, we tested the chosen cell lines in terms of intra-cellular and secreted protein expression. The yellow fluorescent protein (YFP) and a secreted version of the influenza A virus hemagglutinin (HA) subtype H1 representing a complex, glycosylated, pharmaceutically relevant protein served as model proteins. This is the first systematic study that includes validation of insect cell lines for virus titer determination of viruses that have been generated in different cell lines, virus production yields as well as protein expression capacities.

Analysis of susceptibility of cell lines and baculovirus production
Tnao38, High Five and Sf9 cells were infected with a multiplicity of infection (MOI) of 1 with AcNPV-H1-Cal09-YFP. Virus was harvested 6 days post-infection (dpi) and clarified by centrifugation at 1,0009g for 10 min. The obtained virus generated in each cell line was used for subsequent titer determination on all three cell lines via endpoint-dilution assay (Harwood 2007) in 96-well plates. Evaluation was done 6 dpi by fluorescence microscopy.
Recombinant protein expression 9 9 10 5 cells/ml of each cell line were infected with AcNPV-H1-Cal09-YFP at an MOI of 10 in duplicates. Infected cells were maintained in shake-flasks in 50 ml at 100 rpm at 27°C in HyClone SFM4Insect medium (Thermo Fisher Scientific) (Tnao38 (0 % (v/v) FCS) and Sf9 [3 % (v/v) FCS]) or serum-free IPL-41 medium (High Five). Cells from each cell line infected with virus encoding an unrelated protein served as negative control. Growth behaviour of infected cells was monitored, respective total and viable cell numbers were determined via TC20 cell counter (Bio-Rad). Infected cells were harvested every 24 h for 5 days and analyzed in regard to recombinant protein expression. Cell pellets were lysed in 1 ml I-PER Insect Cell Protein Extraction Reagent (Pierce) and YFP expression was measured in a plate reader at 488 nm excitation/520 nm emission. To assess HA expression, supernatant and pellet fractions were analyzed via SDS-PAGE and Western blot. Samples were mixed with 29 electrophoresis buffer containing 0.5 M Tris/HCl, 87 % (w/v) glycerol, 2 % (w/v) SDS, 0.1 % (v/v) Bromphenol Blue, pH 6.8 and 100 mM DTT. Proteins were separated by SDS-PAGE according to Laemmli, stained with Comassie Brilliant Blue G-250 or electroblotted on a PVDF transfer membrane. Membranes were blocked overnight with 3 % BSA in T-PBS (PBS with 0.1 % (v/v) Tween 20). Proteins were detected with an anti-H1-Cal09 polyclonal serum produced in mice, (EF-BIO, s.r.o., Tabaková 2942/5, 811 07, Bratislava, Slovakia) diluted 1:2,000 in T-PBS containing 1 % (w/v) BSA by incubation for 1 h. Followed by 1 h incubation of alkaline phosphatase conjugated antimouse IgG (c-chain specific, produced in goat) antibody (Sigma-Aldrich, A1047) diluted 1:2000 in T-PBS containing 1 % (w/v) BSA, Western blots were developed using BCIP/NBT solutions (Sigma-Aldrich) (50 mg/ml).

Virus-host interactions: analysis of susceptibility of cell lines and production of baculovirus progeny
Based on the observation that insect cell lines differ in susceptibility to AcNPV (Lynn 2003), we wanted to investigate possible differences in virus up-take and titration results when in contrast to the routinely used Sf9 cells, High Five and Tnao38 cells are instead employed for titration. Therefore, all three cell lines were infected with AcNPV-H1-Cal09-YFP generated in Sf9 cells at an MOI of 1. Respective titers were determined via endpoint dilution assay on all three cell lines in parallel (experimental scheme is shown in Fig. 1). Figure 2 shows results for titer determination of virus generated by infection of the different cell lines on Tnao38, High Five and Sf9 cells.
Independent of the cell line employed for virus production, calculated titers were about one log higher when Tnao38 and High Five cells were used for titration as compared to Sf9 cells. Thus, both Trichoplusia ni-derived cell lines displayed higher susceptibility to AcNPV than Spodoptera frugiperda Sf9 cells. Apparently, the producing host cell had no influence on viral up-take in the different cell lines. Furthermore, it was noted that Sf9 cells yielded about 100-fold more infectious particles as compared to Tnao38 and High Five cells, independent which cell line was used for titer determination. Thus, higher susceptibility is not combined with faster or more efficient virus replication or higher virus titer production.

Recombinant protein expression
For validation of the three cell lines Tnao38, High Five and Sf9 in regard to recombinant protein expression, cells were infected at an MOI of 10 with AcNPV-H1-Cal09-YFP. This baculovirus construct was designed to express YFP as an easy detectable protein that is produced intracellularly and a secreted version of influenza A virus hemagglutinin, lacking the transmembrane domain, as a model for a complex glycoprotein. Experiments were performed in duplicates, showing consistent results; representative data is shown for one replica.

Intracellular protein expression
Intracellular YFP yield was detected via fluorescence measurement in a plate reader (Fig. 3).
An increase of the YFP signal was detected in all cell lines 2 dpi. When comparing YFP peak levels, Tnao38 cells showed twofold higher expression than High Five cells and an about threefold higher YFP signal could be detected as compared to Sf9 cells. Overall, YFP expression in Tnao38, High Five and Sf9 cells showed cell line dependent differences, in terms of yield and also regarding time point of production peaks. Additionally cell viability was determined over time for all three cell lines, where Tnao38 cells showed the highest value on day 5 (60 %) in comparison to High Five (16 %) and Sf9 cells (32 %). This may indicate a higher stability of the Tnao38 cell line which has been described previously (Palmberger et al. 2012), suggesting that this may be the cause for higher YFP expression.

Protein secretion
Protein yields and integrity of soluble trimeric influenza A virus hemagglutinin H1-Cal09 were detected by Western blot analysis. Figure 4 shows unsecreted and secreted HA in the cell pellet and in the supernatant of Sf9, High Five and Tnao38 cells, respectively. Strong expression of HA was detected 2-5 days after infection in the cell pellet, for all cell lines. Secreted HA was detected in the supernatant of all cells starting on day 2 throughout to day 5, where significantly higher amounts were found for High Five and Tnao38 cells as compared to Sf9 cells. Yet, some product degradation was observed for secreted HA from Tnao38 cells (Fig. 4, lower panel),  In contrast, all cell pellets resulted in a double band, probably due to uncompleted processing or partial degradation.

Discussion
Production of biopharmaceuticals in insect cells is an emerging field in biotechnology. Cell engineering and vector design for higher expression rates and for providing human-like glycosylation are successful strategies for further advancement of the baculovirus expression system in terms of protein quality (Aumiller et al. 2012;Viswanathan et al. 2005). When it comes to process design and up-scaling strategies, the potential and limits still need to be determined. One major factor is the choice of the insect cell line. While Sf9 cells are the most widely used cell line, High Five cells have shown to be highly feasible for efficient protein secretion (Granados et al. 2007;Krammer et al. 2010). Tnao38 is a recently established cell line (Hashimoto et al. 2010) and only preliminary data about production performance have been published. Therefore, comparative analysis and validation of available insect cell lines is highly important.
Here, we characterized these three cell lines, Tnao38, High Five and Sf9, in terms of baculovirus susceptibility, virus progeny production and in regards to their feasibility to express non-secreted and secreted proteins. Employment of Tnao38 and High Five cells for baculovirus titer determination resulted in a tenfold higher titer as compared to using Sf9 cells, independently which cell line was used for virus propagation (Fig. 2). Thus, employment of Tnao38 and High Five cells provides a more sensitive assay for titer determination, which can be of importance e.g. for the detection of unwanted baculovirus background and for fulfilling regulatory requirements for licensing new baculoviral derived products of medical relevance. According to our findings, Sf9 cells as state of the art cell line used for determination of the baculovirus titer does not reflect the actual number of infectious virus particles, but leads to an underestimation of titer values.
Correct titer results are important for calculating MOIs; infection with more virus than anticipated may increase metabolic burden and cause decreased productivity. Since many processes have been optimized regarding MOI for transduction experiments in mammalian cell culture and gene therapeutic applications, the fact that the titer is about one log higher than estimated according to Sf9 cell based titer determination might be taken under consideration. Possibly, there exist insect cell lines that would give an even higher titer value and our findings may just reflect an approximation of the 'real' baculovirus titer. Also, accurate titer determination based on more susceptible cells may help in the establishment of a baculovirus reference material for inter-and intra-laboratory comparison (Kamen et al. 2011). Sf9 cells are preferably used for basic viral assays, transfections and for the production of high titer baculovirus stocks. In terms of producing baculoviruses, we found Sf9 cells to be 100-fold more efficient. Regarding recombinant protein expression, we found that Tnao38 and High Five cells were more suitable for both intra-and extracellular model proteins. Tnao38 cells showed approximately a 2-and 3-fold higher intracellular YFP expression compared to High Five and Sf9 cells, respectively (Fig. 3). The persistent increase of intracellularly-expressed YFP in Tnao38 cells may indicate that this cell line exhibits higher stability upon baculovirus infection. With regard to expression of the influenza A virus hemagglutinin, Tnao38 and High Five cells were comparable in terms of secretion and overall expression. In contrast to YFP expression experiments, here we found similar expression levels on days 2-5. This might be due to the higher stability of the trimeric HA as compared to YFP. Furthermore, no difference in the course of the expression was observed, suggesting similar properties in robustness. In terms of product quality, High Five cells seem to be superior as compared to Tnao38 cells, since Western blot analysis indicated protein degradation in the supernatant of Tnao38 cells, while HA secreted by High Five cells resulted in only one distinct band. Sf9 cells showed significantly lower amounts of secreted HA in the supernatant as compared to Tnao38 and High Five cells (Fig. 4). Higher protein yields may be attributed to lower metabolic burden due to virus replication and to the higher susceptibility of the two Trichoplusia ni cell lines. To date, still many secreted glycoproteins are generated in Sf9 cells, such as influenza virus HA and various virus-like particles (Cox 2008;Khurana et al. 2011;Liu et al. 2013;Tretyakova et al. 2013). However, our findings suggest the high applicability of cell lines derived from Trichoplusia ni for recombinant protein expression, especially in the case of secreted proteins. Further investigation of virushost interactions and their influence on productivity, as well as tools for reliable quality control of the products, will be necessary to advance the application of insect cells as feasible cell factories.