Abstract
Circular RNAs are emerging players in human cancers, including esophageal squamous cell carcinoma (ESCC). Herein, we assessed the expression level of circ_0023990 and explored the molecular mechanisms of circ_0023990 in ESCC. circ_0023990, miR-6884-5p, and PAK1 expressions in ESCC tissues and cells were detected by quantitative real-time polymerase chain reaction and western blot. ESCC cells were transfected with different constructs to alter the expression of circ_0023990, miR-6884-5p, and PAK1. The effect of circ_0023990 on the proliferation, invasion, and glycolysis of ESCC cells was observed. The interaction between circ_0023990 and miR-6884-5p and between miR-6884-5p and PAK1 were explored. A mouse model of ESCC was established to study the in vivo effect of circ_0023990 knockdown on tumor formation.The expression levels of circ_0023990 was upregulated in ESCC tissues and cells. Inhibiting circ_0023990 suppressed the proliferation, invasion, and glycolysis of ESCC cells. circ_0023990 might target miR-6884-5p and consequently modulate the expression and activity of PAK1. Knockdown of circ_0023990 led to significantly reduced tumor volume and weight in mice with ESCC.These findings overall suggest an oncogenic role of circ_0023990 in ESCC. Future research is warranted to confirm the expression pattern and clinical significance of circ_0023990 in ESCC.
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The datasets generated during and/or analyzed during the current study are not publicly available, but are available from the corresponding author on reasonable request.
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Ningbo Natural Science Foundation(2017A610155). Zhejiang Medical and Health Science and Technology Project(2020KY867). NINGBO Medical & Health Leading Academic Discipline Project(Project Number:2022-F02).
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Conception and design: Jinxian He, Hui Tian, Gaofeng Liang. Data analysis and interpretation: Hui Tian, Gaofeng Liang, Qi Qin. Manuscript writing: Jinxian He, Hui Tian, Chaoqun Yu. Final approval of manuscript: All authors.
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Written informed consent was obtained from each patient included in the study and the study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as reflected in a priori approval by the Ethics Committee of Ningbo Medical Center Lihuili Hospital. Each animal experimental procedure gained approval from Animal Ethics Committee of Ningbo Medical Center Lihuili Hospital. The experimental protocol was performed in accordance with the relevant guidelines and regulations of the Basel Declaration. The study is reported in accordance with ARRIVE guidelines (https://arriveguidelines.org).
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Tian, H., Liang, G., Qin, Q. et al. Circ_0023990 Promotes the Proliferation, Invasion, and Glycolysis of Esophageal Squamous Cell Carcinoma Cells Via Targeting miR-6884-5p/PAK1 Axis. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10674-z
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DOI: https://doi.org/10.1007/s10528-024-10674-z