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Let-7c-5p Down Regulates the Proliferation of Colorectal Cancer Through the MAPK-ERK-Signaling Pathway

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A Correction to this article was published on 21 February 2024

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Abstract

Colorectal cancer (CRC) is one of the most prevalent and life-threatening cancers. Rapid cell proliferation is the leading cause of cancer-related death in CRC. MicroRNAs (miRNAs) have been identified to play essential roles in the proliferation of CRC. Differential expression of let-7c-5p in CRC was assessed using a GEO dataset, and confirmed through RT-qPCR using CRC subject tissues. Let-7c-5p-overexpressing HCT8 cell line was constructed by transfecting let-7c-5p. Bioinformatics analysis identified that DUSP7 is the target gene of let-7c-5p. Further experimental assays, including Cell Counting Kit-8 (CCK8), EdU staining, cell colony, and Western Blot assays, confirmed the target genes and pathway of let-7c-5p. Receiver operator characteristic curve (ROC) analysis was performed to evaluate the diagnostic value of let-7c-5p for CRC. Finally, survival analysis was performed to determine the effect of DUSP7 and let-7c-5p on the prognosis of CRC patients. RT-qPCR analysis showed that the expression level of let-7c-5p was significantly increased in CRC subject tissues compared to the adjacent tissue. Overexpression of let-7c-5p promoted cell proliferation in HCT8 cell line. Furthermore, the MAPK-ERK pathway’s protein expression of p-ERK1/2 was downregulated, while the ratio of Bcl-2/Bax was increased by let-7c-5p transfection in HCT 8. ROC analysis demonstrated that the expressive level of let-7c-5p had higher diagnostic value for CRC. Survival curve analysis indicated that high expression of DUSP7 and low expression of let-7c-5p were associated with poor prognosis in CRC patients. The findings suggest that let-7c-5p exerts an antitumor function by inhibiting the DUSP7-mediated MAPK-ERK pathway. Both DUSP7 and let-7c-5p have the potential to serve as prognostic biomarkers in CRC patients.

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All data are available from the corresponding author on request.

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Acknowledgements

The authors thank the financial support of Hebei Provincial of Human Resources and Social Security as well as Chengde Medical University.

Funding

The study was supported by Hebei Provincial of Human Resources and Social Security (CY201701, CY201702).

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Authors and Affiliations

  1. School of Basic Medical Sciences of Chengde Medical University, Chengde, Hebei, China

    Juanjuan Fu, Jining Zheng & Zhiping Hou

  2. Department of Gastroenterology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China

    Longmei Zhou, Sijin Li & Peiyuan He

  3. Kuancheng Manchu Autonomous County Hospital, Chengde, China

    Wenjing He

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  1. Juanjuan Fu
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  3. Sijin Li
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Contributions

FJJ, HZP and HPY designed the study and wrote the manuscript. LSJ and ZLM carried out data analysis, HWJ prepared pathological slides. All authors have read and approved the manuscript.

Corresponding authors

Correspondence to Zhiping Hou or Peiyuan He.

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The authors declared no potential conflicts of interest concerning the research, authorship, and/or publication of this article.

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The studies involving human participants were reviewed and approved by the Ethics Committee of Chengde Medical University.

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The original online version of this article was revised: The affiliation 'Basic College of Chengde Medical University, Chengde, Hebei, China' has been removed.

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Fu, J., Zhou, L., Li, S. et al. Let-7c-5p Down Regulates the Proliferation of Colorectal Cancer Through the MAPK-ERK-Signaling Pathway. Biochem Genet (2023). https://doi.org/10.1007/s10528-023-10581-9

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  • DOI: https://doi.org/10.1007/s10528-023-10581-9

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