Abstract
TRP channels have an important role in regulating the function of gastrointestinal epithelial cells. The aim of this study was to investigate the molecular mechanisms of genes associated with TRP channels in Crohn's disease (CD) by bioinformatics approach and to identify potential key biomarkers. In our study, we identified TRP channel-related differentially expressed genes (DEGs) based on the GSE95095 dataset and the TRP channel-related gene set from the GeneCards database. Hub genes (CXCL8, HIF1A, NGF, JUN, IL1A) were identified by the PPI network and validated by the external GSE52746 dataset. Immune infiltration analysis revealed that CXCL8 was significantly correlated with B cells memory, NK cells activated, Mast cells resting, Mast cells activated, and Neutrophils. GSEA of CXCL8 results showed inositol phosphate metabolism, RNA polymerase, propanoate metabolism, MAPK signaling pathway, base excision repair, and Calcium signaling pathway. In addition, we constructed a lncRNA-miRNA-mRNA ceRNA network and a drug–gene interaction network. Finally, we performed in vitro experiments to verify that LPS induced CXCL8 expression in HT-29 cells and that knockdown of CXCL8 inhibited the inflammatory stimulatory effects of LPS. This study reveals that CXCL8 plays an important role in the pathogenesis of Crohn's disease and is expected to be a novel biomarker.
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Data Availability
Data supporting the results of this study are available in the article.
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Funding
This study was supported by the Jiangsu Provincial Chinese Hospital Administration (No. ZT202120) and the Jiangsu Provincial Health and Wellness Commission Scientific Research Project (No. M2021032).
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WC and RS conceived and designed the project; ZX and JJ edited this manuscript; WC, LC, and YC performed the experiments; WC analyzed and interpreted the data; TY and HC revised this manuscript. The authors read and approved the final manuscript.
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Chen, W., Xu, Z., Jiang, J. et al. CXCL8 as a Potential Biomarker for Crohn’s Disease is Associated with Immune Infiltration and RNA Methylation. Biochem Genet 61, 2599–2617 (2023). https://doi.org/10.1007/s10528-023-10397-7
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DOI: https://doi.org/10.1007/s10528-023-10397-7