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Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus

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Abstract

Papaya ringspot virus (PRSV) has a single-stranded RNA genome and causes severe economic losses both in cucurbits and papaya worldwide. The extent to which the genome of PRSV is shaped by recombination provides an understanding of the molecular evolution of PRSV and helps in studying features such as host specificity, geographic distribution, and its emergence as new epidemics. The PRSV-P-Indian isolate was completely sequenced and compared with 14 other isolates reported from the rest of the world for their phylogenetic survey of recombination events. Cistron-by-cistron sequence comparison and phylogenetic analysis based on full-genome polyprotein showed two distinct groupings of Asian and American isolates, although PRSV-P and W-India clustered along with the American isolates. Recombination sites were found throughout the genomes, except in the small 6K1 protein gene. A significant proportion of recombination hotspots was found in the P1 gene, followed by P3, cylindrical inclusion (CI), and helper component proteinase (HcPro). Correlations between the presence of recombination sites, geographic distribution, and phylogenetic relationship provide an opportunity to establish the molecular evolution and geographic route of PRSV.

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Acknowledgment

Satendra K. Mangrauthia was supported by a fellowship from the Council of Scientific and Industrial Research, New Delhi.

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Authors and Affiliations

  1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012, India

    Satendra K. Mangrauthia

  2. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    B. Parameswari, R. K. Jain & Shelly Praveen

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  1. Satendra K. Mangrauthia
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  2. B. Parameswari
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  3. R. K. Jain
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  4. Shelly Praveen
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Correspondence to Shelly Praveen.

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Mangrauthia, S.K., Parameswari, B., Jain, R.K. et al. Role of Genetic Recombination in the Molecular Architecture of Papaya ringspot virus . Biochem Genet 46, 835–846 (2008). https://doi.org/10.1007/s10528-008-9198-y

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  • DOI: https://doi.org/10.1007/s10528-008-9198-y

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