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A Canine Surgical Model of Stroke by Minimally Invasive Occlusion of the Proximal Middle Cerebral Artery

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In this study, a model of ischemic stroke by surgical proximal middle cerebral artery (MCA) occlusion was developed on 10 beagle dogs. The advantages of this model are the transtemporal approach and a minimally invasive surgical procedure. Dogs were randomly assigned to two groups: sham-operated (proximal MCA exposure without occlusion) and experimental (permanent proximal MCA occlusion) groups. Different evaluation methods were used to assess the consequences of MCA occlusion in dogs, including neurobehavioral tests, MRI, and immunohistochemical staining. Clear signs of cerebral infarction associated with the region supplied by MCA were confirmed and the model showed good repeatability and consistency. The model can serve as an appropriate large animal model to improve the translation of stroke therapeutics research from the laboratory to the clinical practice.

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Authors and Affiliations

  1. Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China

    J. Liu, X. Xiong & Z. Jian

  2. Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular Research Institute of Wuhan University, Hubei Key Laboratory of Cardiology, Wuhan, Hubei Province, China

    Y. Wang, J. Yin & L. Zi

Authors
  1. J. Liu
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  2. Y. Wang
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  3. J. Yin
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  4. L. Zi
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  5. X. Xiong
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  6. Z. Jian
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Corresponding authors

Correspondence to X. Xiong or Z. Jian.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 9, pp. 361-368, September, 2022

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Liu, J., Wang, Y., Yin, J. et al. A Canine Surgical Model of Stroke by Minimally Invasive Occlusion of the Proximal Middle Cerebral Artery. Bull Exp Biol Med 174, 370–375 (2023). https://doi.org/10.1007/s10517-023-05710-8

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  • DOI: https://doi.org/10.1007/s10517-023-05710-8

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