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Mesenchymal Stem Cells Enhance Chemotaxis of Activated T Cells through the CCL2-CCR2 Axis In Vitro

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Activation and migration of donor T cells to the host target organs are critical mechanisms in the pathogenesis of graft-versus-host disease (GVHD). The role of monocyte chemoattractant protein-1 (MCP-1/CCL2) and its receptor CCR2 in the recruitment of T cells during immune or inflammatory response is also well known. For elucidation of the mechanism of the therapeutic effect of human bone marrow derived-mesenchymal stem cells (MSC) in GVHD, we studied the effect of these cells on migration of activated donor T cells through the CCL2-CCR2 axis in vitro. MSC were expanded from donors’ bone marrow mononuclear cells. After co-culturing of IL-2-activated T cells with allogeneic MSC at different ratios, the levels of CCL2 in supernatants were measured by ELISA, and CCR2 expression in CD4+/CD8+ T cells subsets were detected by flow cytometry. The effect of MSC on the migration of activated T cells in the Transwell system was studied in the absence or presence of CCL2. Our results show that CCL2 levels in supernatants of co-cultures were significantly higher than in MSC monoculture and this increase depended on the number of MSC. MSC inhibited proliferation of T cells, but did not change the percentages of CD4+ and CD8+ T cells subsets. MSC can up-regulate the CCR2 expression in CD8+ subsets rather than in CD4+ subsets; MSC enhanced migration of IL-2-activated T cells to CCL2 by increasing the expression of CCR2. The data demonstrate that MSC can enhance chemotaxis of cytokine-activated T cells through the CCL2-CCR2 axis in vitro.

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Authors and Affiliations

  1. Department of General Practice, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

    Y. L. Zhang

  2. Department of Hematology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

    S. K. Qiao, L. N. Xing, X. N. Guo & J. H. Ren

Authors
  1. Y. L. Zhang
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  2. S. K. Qiao
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  3. L. N. Xing
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  4. X. N. Guo
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  5. J. H. Ren
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Correspondence to S. K. Qiao.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 186-196, September, 2021

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Zhang, Y.L., Qiao, S.K., Xing, L.N. et al. Mesenchymal Stem Cells Enhance Chemotaxis of Activated T Cells through the CCL2-CCR2 Axis In Vitro. Bull Exp Biol Med 172, 263–269 (2021). https://doi.org/10.1007/s10517-021-05373-3

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  • DOI: https://doi.org/10.1007/s10517-021-05373-3

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