Abstract
Clostridium butyricum (CB) has received much attention as a probiotic; however, few studies have focused on its effects on liver health. Here, we studied the influence of CB on the liver health of spotted sea bass through transcriptomics and metabolomics studies and preliminarily explored its molecular mechanisms. This study showed that CB significantly reduced hepatic aspartate aminotransferase (AST) activity and increased alkaline phosphatase (AKP) and acid phosphatase (ACP) activity (P < 0.05). CB has demonstrated significant effects in strengthening liver immunity and can increase hepatic amylase and trypsin activities and promote hepatic catabolism of carbohydrates and amino acids. Integration of the liver transcriptomics and metabolomics showed altered transcript levels of mainly gluconeogenic, lipogenic, and amino acid metabolic pathways. It regulated the abundance of metabolic biomarkers such as arachidonate, crotonyl-CoA, and D-glucose 1-phosphate. Our findings support that CB can reduce liver damage in spotted sea bass, enhance liver immunity, and improve liver metabolism.
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Funding
This experiment was funded by the Science and Technology Planning Project in Fujian, China (Grant No. 2015N0010) and Science and Technology Planning Project in Xiamen, China (Grant No. 3502Z20143017).
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Lumin Kong and Zhongbao Li conceived and designed the experiments.Lumin Kong, Sishun Zhou, Hao Lin, Jianrong Ma, Zhongying Long, Huihui Qin, Zhangfan Huang, Longhui Liu and Yi Lin performed the experiments.Lumin Kong performed the analyses.Lumin Kong analyzed the data, wrote the paper, and prepared figures and tables.Lumin Kong and Zhongbao Li discussed the result together.Zhongbao Li reviewed drafts of the paper.
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Kong, L., Ma, J., Lin, H. et al. Analyzing the influence of Clostridium butyricum on liver health in spotted sea bass (Lateolabrax maculatus) via transcriptomics and metabolomics. Aquacult Int (2024). https://doi.org/10.1007/s10499-024-01398-3
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DOI: https://doi.org/10.1007/s10499-024-01398-3