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High-level expression and characterization of a lipase from Trichosporon fermentans Y3 and its application as an aquafeed additive for grouper (Epinephelus coioides)

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Abstract

A lipase gene was cloned from Trichosporon fermentans Y3, subcloned into pPICZαA, and then expressed in Pichia pastoris X33. The lipase (lipRT), which was obtained using a 50-L bioreactor, had maximum activity of 6000 U/mL. It had an optimum pH 8.0 and remained stable between pH 3.0 and 11.0, while its optimum temperature was 45 °C, maintaining more than 55% of optimum activity between 20 and 35 °C. To evaluate the potential application of lipRT as an aquafeed additive for juvenile grouper (Epinephelus coioides), fish with initial weight 13.31 ± 0.26 g were randomly divided into 12 sea cages and fed five formulated diets (labeled diets 1 to 4). Diets 1–4 were formulated to include 8% crude lipid and serial levels of lipRT (0 U/kg, 50 U/kg, 500 U/kg, and 1000 U/kg). The results showed that WG, FE, and PER of fish as well as ACH50, LSZ, and O2− production ratio in serum of fish on diets 3 and 4 were significantly (P < 0.05) higher than those on diets 1 and 2. The present data suggests that lipRT could improve the utilization of lipid and some nonspecific immunological parameters, and therefore could potentially be used as an aquafeed additive.

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Funding

This project is supported by projects of Guangdong Department of Science and Technology (No. 2011B050300026 and S2011030005257).

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Authors and Affiliations

  1. Marine Biology Institute, Shantou University, Shantou, Guangdong, China

    Tao Zhang, Jude Juventus Aweya & Wenhua Liu

  2. College of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong, China

    Wenju Xu

  3. Key Laboratory of Aquaculture Nutriton and Feed, Ministry of Agriculture, Ocean University of China, Qingdao, Shandong, China

    Shude Xu

  4. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, Shandong, China

    Shude Xu

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Zhang, T., Xu, W., Xu, S. et al. High-level expression and characterization of a lipase from Trichosporon fermentans Y3 and its application as an aquafeed additive for grouper (Epinephelus coioides). Aquacult Int 28, 1279–1292 (2020). https://doi.org/10.1007/s10499-020-00525-0

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