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Marinifaba aquimaris gen. nov., sp. nov., a novel chitin‐degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench

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Abstract

A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench. Strain SM1970T grew at 15-37 oC and with 1–5% (w/v) NaCl. It hydrolyzed colloidal chitin, agar and casein but did not reduce nitrate to nitrite. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria. The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c). The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8. The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation. The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6–57.1% while the percentage of conserved proteins (POCP) values between them were 28.5–31.5%. The genomic DNA G + C content of strain SM1970T was 40.1 mol%. On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).

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Acknowledgements

We sincerely thank Jing-Yao Qu, Jing Zhu and Zhi-Feng Li from State Key Laboratory of Microbial Technology of Shandong University for help and guidance in LC-MS and professor Aharon Oren from the Hebrew University of Jerusalem for his assistance in naming the new genus.

Funding

This work was supported by the National Key Research and Development Program of China (grants 2018YFC0310704 and 2018YFC1406504, awarded to Xiu-Lan Chen and Xiao-Yan Song), the National Science Foundation of China (grants 31971535, U1706207, 42076151, 31900023, 42076229 and 31870101, awarded to Xiao-Yan Song, Yu-zhong Zhang, Xi-ying Zhang, Hai-Nan Su, Chun-Yang Li and Qi-Long Qin, respectively), Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (2019JZZY010817 awarded to Yu-Zhong Zhang), Taishan Scholars Program of Shandong Province (tspd20181203 awarded to Yu-Zhong Zhang). Persons employed by the funders had no role in the study or in the preparation of the article or decision to publish.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China

    Xin Sui, Xiao-yan He, Ning-hua Liu, Yan-ru Dang, Qian-qian Cha, Xiao-yan Song, Qi-long Qin, Xiu-lan Chen, Yu-zhong Zhang, Hai-nan Su & Xi-ying Zhang

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Mei-ling Sun, Chun-yang Li, Hui-hui Fu, Xiu-lan Chen, Yu-zhong Zhang, Hai-nan Su & Xi-ying Zhang

  3. College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, 266003, China

    Mei-ling Sun, Chun-yang Li, Hui-hui Fu & Yu-zhong Zhang

  4. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China

    Hai-nan Su & Xi-ying Zhang

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  1. Xin Sui
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Contributions

Xi-ying Zhang, Yu-zhong Zhang, Xiu-lan Chen designed and coordinated this study; Xin Sui performed experiments and drafted the manuscript; Xiao-yan He, Ning-hua Liu and Mei-ling Sun helped in experiments and interpreting the results; Yan-ru Dang, Qian-qian Cha, Qi-long Qin, Hui-hui Fu and Hai-nan Su collected the samples and helped in experiments; Chun-yang Li, Xiao-yan Song, Xiu-lan Chen, Yu-zhong Zhang, Xi-ying Zhang and Xiao-yan He revised the manuscript.

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Correspondence to Hai-nan Su or Xi-ying Zhang.

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Xin Sui and Xiao-yan He have contributed equally to this work.

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Sui, X., He, Xy., Liu, Nh. et al. Marinifaba aquimaris gen. nov., sp. nov., a novel chitin‐degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench. Antonie van Leeuwenhoek 114, 947–955 (2021). https://doi.org/10.1007/s10482-021-01568-w

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