Comparative analysis of airborne bacteria and fungi in two salt mines in Poland

The aim of this work was to determine the genera or species composition and the number of colony forming units of airborne bacteria and fungi, respectively, in two salt mines in Poland “Wieliczka” (Lesser Poland) and “Polkowice–Sieroszowice” (Lower Silesia). Both of them are working environments characterized by extreme conditions, and additionally “Wieliczka,” officially placed on the UNESCO World Heritage Sites’ list, plays a role of tourist attraction. There are also some curative chambers located in this mine. Air samples were taken once in December 2015, between 6:00 a.m. and 9:00 a.m. There were nine measurement points located about 200 m underground in “Wieliczka” and six measurement points located in the working shafts about 400 m underground in “Polkowice–Sieroszowice.” The total volume of each air sample was 150 L. Air samples, collected in individual measurement points of both salt mines, were inoculated on two microbiological media: potato dextrose agar and tryptic soy agar using the impact method. We identified 10 and 3 fungal genera in the “Wieliczka” Salt Mine and in “Polkowice–Sieroszowice,” respectively. The most common were fungi of the Penicillium genus. In both mines, the Gram-positive bacteria of genus Micrococcus were detected most frequently. Among identified microorganisms, there were neither pathogenic fungi nor bacteria. The most prevalent microorganisms detected in indoor air were Gram-positive cocci, which constituted up to 80% of airborne microflora. Our results showed that microorganisms recorded in the air samples are not a threat to workers, tourists or patients. Neither pathogens nor potentially pathogenic microorganisms, listed as BSL-2, BSL-3 or BSL-4, were detected. The microbes identified during our analysis commonly occur in such environments as the soil, water and air. Some of the detected bacteria are component of natural microflora of human skin and mucous membranes, and they can cause only opportunistic infections in individuals depending on their health condition.


Introduction
The purity of the atmospheric air is one of the basic issues of the evaluation procedure of work space quality. Particular attention should be paid to bioaerosols that are ubiquitous in indoor air and potentially harmful to human health. Although the air is considered to be an inimical environment for the reproduction of microorganisms, they are able to endure there and maintain their infectious skills. Bioaerosol, also referred to as organic dust, is usually composed of pathogenic and non-pathogenic bacteria, viruses, fungal spores, matter of plants and animals as well as their primary and secondary metabolites including the bacterial endotoxins or mycotoxins. They are transmitted through airborne route, droplet route, vector-borne route, by direct contact and less by the oral route. The saprophytic and infectious bioaerosols are considered to worsen hygienic conditions contributing to the human allergy. They may cause a waste of food, construction materials and pharmaceuticals. Bioaerosols are also responsible for transmission of many of human, animal and plant infectious diseases, mycosis, upper respiratory infections, viral diseases, fire blight and many others (Douwes et al. 2003;Górny 2004;Heikkinen et al. 2005; https:// oshwiki.eu/wiki/Bioaerosols_and_OSH).
The wide and regular monitoring of an environmental cleanliness is increasingly carried out for early detection of the potential risk factors for the social wellness. Actually, there are a few publications describing local occurrence of the biological factors in such specific work spaces as the underground salt mines are (Frączek and Górny 2011;Frączek and Kozdrój 2013;Rdzanek et al. 2015), but there are not legal, generally implemented regulations, which would determine suggested limits of airborne bacteria and fungi in extreme work environments.
The salt mines are characterized by a specific microclimate. Brine aerosols have positive impact on the immune system and promote a treatment of various diseases like the upper respiratory ones, allergies, bronchial asthma and many others. Due to the bacteriostatic effect of the salt, the amount of macroand microorganisms in salt mines is significantly lower than on the earth surface. The number of microorganisms in the mine air is also determined by a degree of dust in corridors and chambers, the microclimate conditions and the people staying there (Chervinskaya 2003(Chervinskaya , 2007Hedman et al. 2006;Czajka et al. 2006;d'Obyrn and Rajchel 2014;Rashleigh et al. 2014).
The aim of our study was to evaluate the airborne bacteria and fungi in underground spaces in two salt mine areas in Poland: the ''Wieliczka' ' Salt Mine (Lesser Poland) and the KGHM Polska Miedź S.A. Mine Department ''Polkowice-Sieroszowice'' (Lower Silesia).
As there are no legal regulations in Poland enabling the performance of reliable evaluation of the microbiological quality of the air, in our research we have based on Directive 2000/54/EC, as well as on the suggested limit values for microorganisms in indoor air published by Polish authors (Górny 2004;Zielińska-Jankiewicz et al. 2005;Górny 2010; https:// oshwiki.eu/wiki/Bioaerosols_and_OSH) (Table 1). Actually, the United States Environmental Protection Agency (EPA 1989) does not regulate suggested limits of microbes in indoor air (https://www.epa.gov). Some specific recommendations have been given by various scientific organizations. For example, the recommended maximum limits are: 1000 CFUs/m 3 for the total number of bioaerosol particles set by the National Institute of Occupational Safety and Health (NIOSH), as well as by the American Conference of Governmental Industrial Hygienists (ACGIH) with the culturable count for total bacteria not to exceed 500 CFUs/m 3 (Yassin and Almouqatea 2010).
Basic principles that mainly concern the prevention or reduction of biological contaminants have been recommended by Environmental Health Directorate in Canada (1987), European Economic Community (EEC 1990) andWorld Health Organization (Bonadonna andMarboni 1994). However, some regulations concerning filamentous fungi have been presented in Report on a World Health Organization meeting in 1988 (WHO 1990).

Description of sampling points
This study was performed in two Polish salt mines with different functions and microclimate conditions ( Table 2). The ''Wieliczka'' Salt Mine, located in Wieliczka city (Lesser Poland), has been producing table salt from thirteenth century until 2007 and now is one of the major tourist attractions in Poland playing a role of a cultural monument, as in 1978 it was officially placed on the UNESCO World Heritage Sites' list. ''Polkowice-Sieroszowice,'' located in Polkowice city (Lower Silesia) is a modern industrial salt mine resulting from the merger in 1996 of two existing mines: ''Polkowice'' and ''Sieroszowice.'' Air samples were collected once-on December 7, 2015 (in ''Wieliczka'') and on December 14, 2015 (in ''Polkowice-Sieroszowice''), between 6:00 a.m. and 9:00 a.m. The sampling points where located about 200 m underground in ''Wieliczka'' and in the working shafts about 400 m underground in ''Polkowice-Sieroszowice.'' There were 9 sampling points in ''Wieliczka'' Salt Mine, consecutively numbered: 1 and 2 located in rehabilitation-treatment chambers (Eastern Mountains' Stable, Chamber, Lake Wessel Chamber), 3-6-located on tourist routes (Chamber Modena, Chapel of St. Kinga, Drozdowice Chamber, Gregory fore-shaft), as well as at the ones (7-9) closed to tourist traffic (Tworzyjanków and Daniłowicz Shaft, Franciszek fore-shaft).
There were 6 sampling points in the industrial salt mine ''Polkowice-Sieroszowice'': 1-situated in the processing plant (the place where the minor repair works of the mining machinery are carried out), 2 and 3-located in Working face I and Working face II ''Najdek'' (the locations where combined mining reaches the solid rock), 4-at the Inflow (the place where an atmospheric air reaches the interior of the corridor), 5-at the Vent (the places where the ventilation ducts are connecting and leaving corridors) and 6-located in Miners' room (the only social place for personnel). The measurement points, besides Vent (Table 4), were located inside of mines.

Microbiological analysis of bioaerosols
The volume of 50 L of each mine-air sample (total volume collected in each measurement point was 150 L) was inoculated, in three replicates, on two microbiological media: PDA (Potato Dextrose Agar, Difco) and TSA (Tryptic Soy Agar, bioMérieux SA, France) for the isolation of fungi and bacteria, respectively, using the impact method and the Air Ideal 3P sampler. TSA medium was previously supplemented with 30 lg/mL of nystatin (Polfa, Kraków) to inhibit the growth of molds and yeast. The plates with TSA were incubated in 37°C for 1 day, and in 22°C for next 3 days. The samples inoculated on PDA were incubated in 30°C for 4 days, and in 22°C for next 4 days. After incubation, the number of visible Table 1 Limit values for microorganisms in indoor air recommended by team of experts of biological factors (ZECB) in Poland (Górny 2004(Górny , 2010 Working rooms contaminated by organic dust a (CFU/m 3 ) Living areas and public spaces (CFU/m 3 ) Mesophilic bacteria 1 9 10 5 5 9 10 3 Gram-negative bacteria 2 9 10 4 2 9 10 2 Thermophilic actinomycetes 2 9 10 4 2 9 10 2 Fungi 5 9 10 4 5 9 10 3 Microorganisms listed as BSL-3 or BSL-4 (pathogens) 0 0 a Suggested values should be two times lower for respirable fraction, i.e., 50,000 CFU/m 3 for mesophilic bacteria, 10,000 CFU/m 3 for Gram-negative bacteria, 10,000 CFU/m 3 for thermophilic actinomycetes, 25,000 CFU/m 3 for fungi colonies of bacteria and fungi was counted and a general number of colony forming units (CFU) was calculated for each of this group separately. The bacterial colonies were divided into morphological groups according to the macroscopic observation on TSA medium. The representative colonies from each group were subsequently inoculated on TSA medium using a steak plate method. Finally, the pure cultures were identified morphologically, by Gram and spore staining, as well as biochemically, by catalase and API tests (bioMérieux SA, France). The fungi, grown on PDA medium, were classified to species using the diagnostic keys based on their macroscopic and microscopic morphology (Germain and Summerbell 1996;Fisher and Cook 1998;Samson et al. 2004).
The concentration of the tested bioaerosol (''X'') was presented as the number of colony forming units (CFU) in 1 m 3 of air. It was determined according to the formula: X = (a 9 1000)/V, where ''a'' is the average number of CFU and ''V'' is a volume of the inoculated air samples (50 L for each plate). Finally, they were compared to guidelines of Team of Experts of Biological Factors (ZECB) in Poland (Górny 2004(Górny , 2010; https://oshwiki.eu/wiki/Bioaerosols_ and_OSH).

Microbiological analysis of air in ''Wieliczka'' salt mine
The concentration of bacteria and fungi and the percentage of individual genera and/or species, identified in air of the ''Wieliczka'' Salt Mine in the selected measurement points, are presented in Table 3. It varied in the tested sampling points. The number of bacteria and fungi ranged from 60 ± 17 to 2027 ± 40 CFU/m 3 of air and from 50 ± 10 to 750 ± 107 of CFU/m 3 of air, respectively. The highest concentration of microorganisms (bacteria and fungi together) was observed in curative chambers (Eastern Mountains' Stable Chamber and Lake Wessel Chamber). In these measurement points, the bacteria were a dominating group (96 and 81%, respectively). The highest concentration of fungal spores was detected in tourist chambers: Chapel of St. Kinga (750 ± 107 CFU/m 3 ) and Chamber of Modena (600 ± 78 CFU/m 3 ). The lowest number of both groups of the microorganisms was observed in chambers closed for tourists, like Franciszek fore-shaft, Daniłowicz and Tworzyjanków Shaft. In Daniłowicz Shaft, the total number of bacteria was estimated at 93 ± 31 CFU/m 3 , whereas the concentration of fungal spores in 1 m 3 was 4 times higher (360 ± 68 CFU/m 3 ) in comparison with bacteria.
The dominant group of bacteria, in tested samples of the air, were Gram-positive cocci belonging to Micrococcus genus. In two curative chambers: Eastern Mountains' Stable and Lake Wessel these cocci were accounted for approximately 67% of the all identified microorganisms (both bacteria and fungi). The staphylococci were also detected in tested samples of the air; however, their number usually did not exceeded 5%. Among them, there were identified Staphylococcus saprophiticus and S. epidermidis. In the tested air samples, collected in the ''Wieliczka'' Salt Mine, 17 species of molds belonging to 9 genera were identified. The most frequent were fungi of the Penicillium genus, particularly P. notatum, P. meleagrinum and P. waksmani. The Penicillium was dominating genus of mycobiota in Gregory fore-shaft (66%) and Chapel of St. Kinga (61%). However, in Daniłowicz Shaft, which is closed for tourists, the dominating species, accounted for 71% of total number of microorganisms, was Cladosporium cladosporoides.

The microbiological analysis of the air samples in ''Polkowice-Sieroszowice'' salt mine
The estimated concentration of bacterial and fungal aerosols and the percentage of individual genera and/ or species in the tested air samples of salt mine in ''Polkowice-Sieroszowice'' are presented in Table 4   ranging from 75 to 90%. There were also detected few (less than 5%) staphylococci. Like in the air of the ''Wieliczka'' Salt Mine, in these aerosols there were identified the single colonies of the Actinomyces, Gram-negative rods and spore-forming bacilli. Apart from bacteria, there were also presented 10 species of molds belonging to 3 genera among which Penicillium spp. was dominated and was the most frequently isolated from an air inlet to the mine.

Discussion
In the literature, little is known about bioaerosol composition in indoor air of the mines. Some of these places are not only workplaces but also touristic attractions, as well as the place where some people are cured Grzyb and Frączek 2010;Frączek and Górny 2011;.
In In recent years, the interest in biological infectious agents that occurs in the air of public and working spaces has increased (Kubera et al. 2015;Frączek and Grzyb 2010;Frączek and Górny 2011;. The fungal spores, as well as the bacteria producing endospores, slime, and pigments are undoubtedly more resistant to unfavorable environmental factors. The microorganisms in indoor air are less exposed to such factors as the temperature fluctuations, atmospheric precipitation, UV radiation which is why they are able to survive longer and their amount does not fluctuate seasonally (winter/summer) as much as it does in outdoor air (Chmiel et al. 2015).
The microbiological components of bioaerosols significantly influence on air quality. The airborne bacteria and fungi can be harmful for human health as Table 3 continued All fungi (%) That is why an efficient monitoring of bacteria and fungi's occurrence in indoor air of diverse environments is a very important issue.
The objects of this study in the ''Wieliczka'' Salt Mine were the chambers utilized curatively, visited by tourists as well as closed for public. In the salt mine ''Polkowice-Sieroszowice,'' the air samples were taken from the places of salt mining, ventilation inlet and outlet and miners' chamber. The culture method, used in this research, has many limitations mentioned by different authors (e.g., Š tursa et al. 2009;Vartoukian et al. 2010;Rastogi and Sani 2011) and the most important of them is that it underestimates the real amount of microbes in the tested samples because a vast majority of microorganisms ([ 99%) are noncultivable and they could be detected only by molecular methods. In case of cultivable microbes, the single colonies visible on plates can be clumps of bacterial and fungal cells and the ones with different nutritional and environmental requirements will not grow together (on common medium and growth conditions). The results of microbiological analysis showed that the concentration of the bacteria and fungi in the air of the both tested mines meets the microbiological standards for air quality proposed by the Team of Experts of Biological Factors (ZECB) (Górny 2004(Górny , 2010; https://oshwiki.eu/wiki/Bioaerosols_ and_OSH). In the ''Wieliczka'' Salt Mine, the highest number of microorganisms (2120 CFU/m 3 of air) was recorded in air sampled in Eastern Mountains' Stable Chamber designated for curative purpose. In the salt mine ''Polkowice-Sieroszowice,'' the highest concentration of microorganisms (3827 CFU/m 3 of air) was observed in salt mining area-Working face I.
The appropriate microbial quality of the air, which was observed in our analysis, may be connected with specific atmospheric conditions in the tested mines. In mid-2011, in the ''Polkowice-Sieroszowice'' salt mine the project of salt extraction on the level of 1 million tons per year was implemented and this task has already been realized in the middle of 2013 making KGHM Polska Miedź the leader of salt producer in Poland. In 2015 it was extracted there almost 234 thousand tons. The low humidity of the air combined with a high temperature recorded in the salt mining area, as well as a high concentration of the salt dust, significantly slows down an excessive growth of microorganisms. In the tested period, the air temperature in the ''Polkowice-Sieroszowice'' was 38 ± 1°C and the air humidity ranged from 15 to 20%. In tested curative chambers of the ''Wieliczka'' Salt Mine, such as the Lake Wessel and the Eastern Mountains' Stable, the aerosol contains the ions of chlorine, sodium, magnesium and calcium; the air is characterized by unique bacteriological purity, the constant temperature 10-12°C and high humidity about 80-90% (d' Obyrn and Rajchel 2014). During our experiments, the average temperature in this mine was 12-15°C and air humidity ranged from 40 to 60%.
In indoor air, microbes are usually distributed unevenly (Yassin and Almouqatea 2010) and in ''Wieliczka'' Salt Mine we observed the differences in number of bacteria and fungi between measurement points. It ranged from 60 to 2027 CFU/m 3 of air (bacteria) and from 50 to 750 of CFU/m 3 of air (fungi). Bioaerosols in the ''Wieliczka'' Salt Mine were characterized by greater biodiversity in comparison with the ''Polkowice-Sieroszowice'' where bacteria were the main microbiological component (about 97% of total isolated microorganisms). Górny and Dutkiewicz (2002) have cited results of the complex study of indoor air, in which the bioaerosol composition of more than 100 flats in 15 towns, located in Upper Silesia conurbation in Poland, was examined. In the group of Gram-positive bacteria, mainly Micrococcus/Kocuria spp., Staphylococcus spp. (in 100% of the tested flats), Bacillus spp. (in 90%) and Nocardia spp. (in 33%) were detected. Among Gram-negative bacteria, the species of the Pseudomonadaceae family (detected in 80% of the examined flats), were dominated. In this study, the Gram-positive cocci Micrococcus spp. dominated in indoor air of both mines. They accounted for 84 and 60% of the total number of microorganisms in ''Polkowice-Sieroszowice'' and in ''Wieliczka,'' respectively. They commonly occur in natural environment and colonize the surface of human skin. Usually, they are not pathogenic; however, some of them can cause opportunistic infections in certain groups of patients, like those with immunodeficiency (Greenblatt et al. 2004;Murray et al. 2011). Fraczek et al. (2013, who studied the bioaerosol components at the Bochnia Salt Mine Health Resort, also observed that Gram-positive cocci prevailed in indoor air samples and Micrococcus spp. accounted for 72% of bacterial and fungal flora. The percentage of other Gram-positive bacteria, like staphylococci, endospore-forming and non-sporulating rods in their study, was also low and ranged from less than 1-4% for individual groups. It was consistent with our analysis, in which the calculated percentage of these microorganisms in both salt mines was lower than 5%. Among the staphylococci there are important human pathogens, particularly Staphylococcus aureus, S. epidermidis, S. haemolyticus or S. saprophyticus, which are etiological factors of the staphylococcal food poisoning, infections of skin and wounds, respiratory and urinary system as well as septicemia resulting from blood system infections (Kloos and Bannerman 1999;Talaro and Talaro 2002;Otto 2009;Peacock 2006;Murray et al. 2011). In this study, the percentage of individual staphylococci in both salt mines did not exceed 5% of the total amount of bacteria and fungi. Among them we detected: S. epidermidis, S. hominis, S. saprophyticus and S. xylosus. The percentage of individual staphylococci was similar in the study performed at the Bochnia Salt Mine Health Resort , but authors identified 8 species of bacteria from this genus, and they did not detect S. saprophyticus. It should be noted that we isolated S. equorum in the ''Wieliczka'' Salt Mine-species that is a natural component of the biota of the healthy horses' skin and it was also isolated from naturally fermented products, e.g., sausages or cheese (Schleifer et al. 1984;Kloos and Bannerman 1999;Leroy et al. 2009; https://en.wikipedia.org/wiki/Staphylococcus_ equorum). It occurred (less than 2%) only in Eastern Mountains' Stable Chamber-formerly authentic stable, where the horses were working in twentieth century and the last one left the underground in 2002 (Gierlotka 2014). Currently it plays a role of curative space.
In the individual tested air samples of both mines less than 100 CFU/m 3 of air of Gram-negative bacteria were isolated, while their acceptable number in respirable fraction of this type of working area is 1000 CFU/m 3 of air (Górny 2010).
As described in the literature, fungi are the dominant group representing up to 40% of total microorganisms detected in bioaerosols. The most common are fungi belonging to Cladosporium, Alternaria, Penicillium, Fusarium, Trichoderma and Mucor genera (Pusz et al. 2013(Pusz et al. , 2014. The representatives of Penicillium and Aspergillus genera are the most dominant in indoor air, while outdoor bioaerosols mainly contain Cladosporium spp. (Cabral 2010;Visagie et al. 2014). According to the results of microbial analysis of indoor air presented by Górny and Dutkiewicz (2002), fungi of the Penicillium and Aspergillus genera, as well as yeast, were detected in most of examined flats in Poland (in 97, 62 and 52%, respectively). The general percentage of fungi in this study ranged from 4 to 79% in the ''Wieliczka'' Salt Mine and from 0 to 11% in the ''Polkowice-Sieroszowice'' salt mine. Primarily the fungi belonging to Penicillium genus were detected. In the ''Wieliczka'' Salt Mine, we identified 8 species of Penicillium spp. and most of them represented P. notatum, P. melagrinum and P. waksmani species. Aspergillus niger, isolated in this research, was the only representative of the Aspergillus genus and it was detected only in Inflow (\ 1% of bacteria and fungi together) in ''Polkowice-Sieroszowice'' mine. Yeast colonies were observed only in Tworzyjanków Shaft (3% of bacteria and fungi together) located in the ''Wieliczka'' Salt Mine.
The fungi are one of the most significant groups of inhalant allergens. The strong allergens are spores produced by as Alternaria spp., Cladosporium spp., Fusarium spp., Mucor spp. or Aspergillus spp. (Pusz et al. 2014). The fungi can also cause superficial and systemic mycoses in immunosuppressed patients. The latter ones affect greater amount of tissues, whole organs or group of organs and the most common are respiratory mycoses (Venarske and deShazo 2002). However, the fungi which were detected in both mines in this study are considered to be safe for human health and some of them are listed as BSL-1 (BioSafety Level-1). Neither pathogenic species Aspergillus fumigatus nor toxigenic ones Stachybotrys atra were detected. Both species are listed as unacceptable in Exposure guidelines for residential indoor air quality (EHD 1987).
Microorganisms belonging to higher levels of BSL involving microbes that pose moderate hazards to personnel and the environment (BSL-2) or may cause serious or potentially lethal disease (BSL-3 and BSL-4) have not been isolated. Microorganisms identified during the analysis presented in this paper are saprotrophs and their law potential to cause diseases makes them to be a little threat for human health. They commonly occur in soil, water and air. Some of the detected bacteria are component of natural microflora of skin and mucous membranes, and they can cause immunopathogenic reactions in individuals depending on their health condition, genetic predisposition, acquired immunity as well as on time of exposition or the number of introduced microorganisms (Szczuka et al. 2013).
In the tested air the Actinomyces have also been recorded. These microorganisms are widespread in soil but they could be detected in air as they are important contamination of working and living environment. Their occurrence, in closed spaces, is connected with increased air humidity (Frączek and Kozdrój 2013). In this research they were detected mainly in the ''Wieliczka'' Salt Mine and their highest concentration was observed in Tworzyjanków Shaft (15%); however, their percentage in total amount of bacteria was low and accounted for 2%.
The bioaerosol composition in salt mines may promote the osmotolerant microbes like Micrococcus spp., Bacillus spp. or Penicillium spp. (Solomon and Viswalingam 2013;Roohi et al. 2012). In fact, the representatives of the Micrococcus genus were the most frequently detected microorganisms in all samples collected in the ''Polkowice-Sieroszowice'' salt mine (75-90%) as well as in both curative chambers of the ''Wieliczka'' Salt Mine such as Eastern Mountains' Stable Chamber (68%) and Lake Wessel (66%). However, the percentage of the endospore-forming bacilli in these mines was negligible ranging from 0 to 2% and from 0 to 5%, respectively. The molds of the Penicillium genus were the most frequently isolated fungi in both salt mines and predominating microorganisms in Chapel of St. Kinga (66%) and Fore-shaft Grzegorz (66%) located on touristic route but in the ''Polkowice-Sieroszowice'' mine its percentage ranged from 0 to 13% of total microbes. The results of the study performed by Gunde-Cimerman et al. (2006) and Marbaniang and Nazareth (2007) showed that not only spores but also mycelium of some species of Penicillium are able to survive a prolonged suspension in brines and they are more plentiful in colder environments, so the temperature conditions in the Wieliczka's curative chambers could be conductive for this group of fungi.
In conclusion, the bacterial and fungal aerosol concentration in the tested air of the both salt mines meets the microbiological standards recommended by the Team of Experts of Biological Factors (ZECB) in Poland (Górny 2004(Górny , 2010; https://oshwiki.eu/wiki/ Bioaerosols_and_OSH). Both the number and the quality of microorganisms recorded at this time in the air samples are not a threat to workers, tourists or patients. Neither pathogens nor potentially pathogenic microorganisms, listed as BSL-2, BSL-3 or BSL-4, were detected. The microbes identified in our analysis commonly occur in such environments as the soil, water and air. Some of the detected bacteria are component of natural microflora of human skin and mucous membranes, and they can cause only opportunistic infections in individuals depending on their health condition. Finally, it needs to be highlighted that in our comparative analysis only one time sampling was performed and only regular monitoring of bioaerosols would provide more information on the occurrence of microorganisms in these mines.
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