Abstract
Objective
The aim of the study was to investigate the effect of c-Jun N-terminal protein kinase (JNK) signaling pathway on influencing the sensitivity to radiotherapy of human nasopharyngeal carcinoma CNE cells.
Methods
Human nasopharyngeal carcinoma CNE multicellular spheroids (MCS) were constructed with three dimensional cell culture methods. Western blot was employed to analyze the activity of JNK signaling pathway in MCS after X-ray irradiation, and the expression of caspase-3 protein before and after using SP600125 (a special inhibitor of JNK). X-ray induced cell apoptosis in MCS before and after treated with SP600125 were detected by TUNEL.
Results
The level of JNK phosphorylation in MCS was a dynamic course after radiation, and there was a phosphorylation peaks at 2 h later, the apoptotic rate of MCS (P < 0.05) and the expression of caspase-3 protein (P < 0.05) were significantly increased after treated with SP600125.
Conclusion
The transient activation of JNK played a important role in sensitivity to radiotherapy of CNE MCS via mediating survival signals, blocking this pathway accelerate cell apoptosis, which may be related to the increased expression of caspase-3.
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Wei, L., Mei, B. Role of JNK signaling pathway in sensitivity to radiotherapy of nasopharyngeal carcinoma. Chin. -Ger. J. Clin. Oncol. 11, 279–281 (2012). https://doi.org/10.1007/s10330-011-0951-z
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DOI: https://doi.org/10.1007/s10330-011-0951-z