Abstract
Sirtuins (SIRTs) are a family of proteins involved in the metabolic process responsible for extending the lifespan. The role of SIRT1, 6, and 7 in oral squamous cell carcinoma (OSCC) and oral leukoplakia (OLP), one of its precursors, is still elusive. In this study, 82 OLP and 77 OSCC were immunohistochemically examined for SIRT1, 6, and 7. Stained sections were thoroughly scanned and evaluated using a digital image analysis program. The SIRT1, 6, and 7 expressions were detected in the nuclei of epithelial and carcinoma cells in various degrees. Afterward, any correlations among SIRTs, including associations with clinicopathological features and the Kaplan–Meier curves were analyzed. OSCC demonstrated significantly higher SIRT1 expression than OLP, while non-dysplastic lesions showed significantly higher SIRT6 expression than other lesions. A strong correlation was observed between SIRT6 and 7 in OLP, SIRT1 and 6 in in OSCC and in SIRT6 and 7 when all lesion types were considered. There were no significant differences between SIRTs reactivity and the clinical features in OLP. For OSCC, SIRT1 and 6 was found to be directly associated with site of the lesion, while SIRT7 showed a direct relationship between gender, stromal lymphocytic infiltration, and depth of the invasion. OSCC with high SIRT7 expression revealed a slightly lower survival probability, although not statistically significant (p = 0.1019). Our findings suggest that SIRT1, 6, and 7 may play correlated and diverse roles in the development and advancement of OSCC.
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15 June 2023
A Correction to this paper has been published: https://doi.org/10.1007/s10266-023-00825-w
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The study protocol was reviewed and approved by the Research Ethics Committee of Tohoku University Graduate School of Dentistry (2020-3-043).
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The original online version of this article was revised as bold style was missing in some significant values available in Table 1, 3 and corrected in this version. In addition, description of Table 1, 3 were incorrect and corrected now. Finally, statement regarding SIRT7 expression were incorrect under the section “Results”, “Discussion” and corrected in this version.
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Sripodok, P., Saito, H., Kouketsu, A. et al. Immunoexpression of SIRT1, 6, and 7 in oral leukoplakia and oral squamous cell carcinoma. Odontology 112, 221–229 (2024). https://doi.org/10.1007/s10266-023-00816-x
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DOI: https://doi.org/10.1007/s10266-023-00816-x