Preservation of kidney function irrelevant of total kidney volume growth rate with tolvaptan treatment in patients with autosomal dominant polycystic kidney disease

Background Tolvaptan slowed the rates of total kidney volume (TKV) growth and renal function decline over a 3-year period in patients with autosomal dominant polycystic kidney disease (ADPKD) enrolled in the Tolvaptan Efficacy and Safety in Management of Autosomal Dominant Polycystic Kidney Disease and Its Outcomes (TEMPO) 3:4 trial (NCT00428948). In this post hoc analysis of Japanese patients from TEMPO 3:4, we evaluated whether the effects of tolvaptan on TKV and on renal function are interrelated. Methods One hundred and forty-seven Japanese patients from TEMPO 3:4 were included in this analysis (placebo, n = 55; tolvaptan, n = 92). Tolvaptan-treated patients were stratified into the responder group (n = 37), defined as tolvaptan-treated patients with a net decrease in TKV from baseline to year 3, and the non-responder group (n = 55), defined as tolvaptan-treated patients with a net increase in TKV. Results Mean changes during follow-up in the placebo, responder, and non-responder groups were 16.99%, − 8.33%, and 13.95%, respectively, for TKV and − 12.61, − 8.47, and − 8.58 mL/min/1.73 m2, respectively, for estimated glomerular filtration rate (eGFR). Compared with the placebo group, eGFR decline was significantly slowed in both the responder and non-responder groups (P < 0.05). Conclusion Tolvaptan was effective in slowing eGFR decline, regardless of TKV response, over 3 years in patients with ADPKD in Japan. Treatment with tolvaptan may have beneficial effects on slowing of renal function decline even in patients who have not experienced a reduction in the rate of TKV growth by treatment with tolvaptan. Supplementary Information The online version contains supplementary material available at 10.1007/s10157-020-02009-0.


Introduction
Autosomal dominant polycystic kidney disease (ADPKD) is an inherited disease characterized by age-dependent development of multiple cysts in the kidneys, causing a gradual and irreversible expansion of kidney volume [1]. ADPKD eventually leads to end-stage renal failure and the need for renal replacement therapy in a majority of patients, typically by the fifth or sixth decade of life [2]. Natural history data on the gradual expansion of total kidney volume (TKV) in ADPKD are available, including 8 years of follow-up from the prospective, observational Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP) I and II studies [1,3,4]. TKV and glomerular filtration rate (GFR) are markers of ADPKD progression, with TKV used 1 3 as a prognostic indicator early in the disease course, prior to significant changes in renal function [4]. Tolvaptan, a vasopressin V 2 -receptor antagonist, suppresses binding of vasopressin to the V 2 -receptor in renal epithelial cells, and vasopressin V 2 -receptor blockade has been shown, in animal models of ADPKD, to inhibit the growth of renal cysts and the decline in renal function [5][6][7][8][9]. In the Tolvaptan Efficacy and Safety in Management of Autosomal Dominant Polycystic Kidney Disease and Its Outcomes (TEMPO) 3:4 clinical trial, a global, multicenter study that included patients from Japan, tolvaptan slowed the rates of TKV growth and renal function decline over a 3-year period compared with placebo in patients with ADPKD [10,11].
In this post hoc analysis of patients enrolled in TEMPO 3:4 in Japan, we assessed the patterns of TKV response to tolvaptan in ADPKD and explored the factors potentially predictive of TKV response, such as baseline ADPKD risk class [12]. Further, we evaluated possible correlations between the effects of tolvaptan on TKV growth rate and on renal function decline.

Study design
This study is a post hoc analysis of the Japanese population from TEMPO 3:4, a multicenter, double-blind, placebocontrolled, 3-year clinical study (ClinicalTrials.gov number, NCT00428948) [10,11]. Patients were enrolled at 129 sites worldwide. Study design, setting, data collection, and enrollment criteria have been previously described in detail [10,13]. Briefly, eligible patients were aged 18-50 years, with a diagnosis of ADPKD by the Ravine criteria, TKV ≥ 750 mL as measured by magnetic resonance imaging (MRI), and estimated creatinine clearance (Ccr) ≥ 60 mL/min. Patients were randomly assigned in a 2:1 ratio to receive tolvaptan or placebo. Study drug was titrated from a daily split dose of 45/15 mg-60/30 mg and 90/30 mg based on patient-reported tolerability. After the titration phase, patients took the highest dose tolerable for 36 months. The primary endpoint was the annual rate of change in TKV from baseline; secondary endpoints included a composite of time to clinical progression (defined as worsening kidney function, kidney pain, hypertension, and albuminuria) and rate of kidney function decline.
TEMPO 3:4 was conducted in accordance with the ethical principles originating in the Declaration of Helsinki and in compliance with good clinical practice guidelines. The protocol was approved by the institutional review board at each trial site. Written informed consent was obtained from all participants.

Objectives
The objectives of this post hoc analysis were to compare the change in TKV from baseline to year 3 among the placebo, responder, and non-responder groups; identify the factors predictive of decrease in TKV; and explore the correlations between changes in TKV and kidney function.

Patients
Patients who completed the TEMPO 3:4 trial in Japan were stratified into three groups: (1) placebo-treated patients; (2) responders, defined as tolvaptan-treated patients with a net decrease in TKV from baseline to year 3; and (3) non-responders, defined as tolvaptan-treated patients who showed a net increase in TKV.

Statistical analysis
The proportions of patients with a net decrease in TKV from baseline to year 3 in the placebo and tolvaptan groups 1 3 were compared by the chi-square test. Factors affecting decrease in TKV were analyzed by univariate logistic regression, with calculation of odds ratio (OR), 95% confidence interval (CI), and P values. The covariates analyzed included demographic characteristics, stratification factors, medical history, current medication, polycystic kidney disease characteristics, and kidney function parameters. Factors found to be significant were further analyzed in a multiple logistic regression model.
To evaluate the correlations between changes in TKV and changes in kidney function parameters from baseline to year 3, Pearson's correlation coefficient (r), simple regression analysis parameter estimate, standard error, 95% CI, and P value were calculated. Changes in TKV, kidney function, and urine osmolality from baseline to year 3 were compared among the placebo and tolvaptan groups using Tukey-Kramer's honestly significant difference test.
Statistical significance was defined as P < 0.05. All statistical analyses were performed using SAS 9.4 and JMP 13 (SAS Institute, Cary, North Carolina).

Patient characteristics
One hundred and forty-seven Japanese patients from the TEMPO 3:4 trial were included in this analysis (placebo, n = 55; tolvaptan, n = 92). By ADPKD risk classification, 9.5% of patients were class 1B, 40.8% class 1C, 30.6% class 1D, and 16.3% class 1E. Tolvaptan-treated patients were stratified into responders (n = 37), defined as patients who achieved a net decrease in TKV from baseline to year 3, or non-responders (n = 55), defined as patients who experienced a net increase in TKV from baseline to year 3 ( Fig. 1). Patient characteristics and demographic data in the placebo, responder, and non-responder groups are shown in Table 1. The distribution of patients in each risk subclass was similar between the placebo and tolvaptan groups (Fig. 2). Overall, 40.2% (37/92) of tolvaptan-treated patients, compared with 5.5% (3/55) of placebo-treated patients, experienced a net decrease in TKV from baseline to year 3 (P < 0.0001). Tolvaptan was associated with decreased TKV in 60% of

Change in TKV
The time course of percent change in TKV at each visit is shown in Fig. 3 for each group. Changes in TKV from baseline to each year of follow-up were significantly different between the placebo and responder groups (P < 0.0001) and between the responder and non-responder groups (P < 0.0001). No statistically significant differences between the placebo and non-responder groups were observed. In the responder group, TKV was decreased in all patients at 12 months, with the decreases maintained throughout 3 years of treatment in all patients, with the exception of one patient at year 2 ( Fig. 4).
Data from additional analyses for changes in eGFR in class 1 patients and by age are summarized in Online Resources 1 and 2.

Associations between TKV and renal function
In the placebo group, changes in Cr (r = 0.5971; P < 0.0001) and CysC (r = 0.5240; P < 0.0001) from baseline to year 3 demonstrated a significant positive correlation with TKV, while Ccr (r = − 0.3110; P = 0.0208) and eGFR CKD-EPI (r = − 0.2811; P = 0.0376) showed a significant negative correlation (Table 5). However, no statistically significant associations between changes in TKV and renal function were observed in either the responder or the non-responder group.

Discussion
Mechanisms of cyst expansion in ADPKD and the associated increase in TKV are based on reduced intracellular Ca 2+ influx caused by mutations in either PKD1 or PKD2, increased cellular adenosine 3′, 5′-cyclic monophosphate (cAMP) levels, and aberrant Ras/Raf/ERK activation [18]. Tolvaptan blocks the vasopressin V 2 -receptor, reducing cAMP levels [18]. The progression of ADPKD is characterized by a continuous expansion of cyst volume, a process that was well represented by change in TKV over time in the placebo group of this study (Fig. 3). During longitudinal follow-up in CRISP II, htTKV and GFR exhibited year 3. P < 0.0001 based on Tukey-Kramer's honestly significant difference test comparing percent change in TKV from baseline to each year of follow-up between the placebo and responder groups (*) and between the responder and non-responder groups ( † ). TKV total kidney volume Fig. 4 Time course of TKV in each patient throughout the treatment period. Responders: tolvaptan-treated patients with a net decrease in TKV from baseline to year 3. Non-responders: tolvaptan-treated patients with a net increase in TKV from baseline to year 3. TKV total kidney volume a significant negative correlation [4]. This phenomenon has also been observed in a Japanese cohort, in which a significant negative correlation between baseline TKV and eGFR slope was observed [19]. Thus, TKV is used as a surrogate biomarker of ADPKD progression [1]. In our study, female sex was a significant positive predictor of tolvaptan's effect in reducing TKV growth. Since there was no modification of tolvaptan dosage for either sex in this study, it is possible that the same dosage may have produced higher serum drug concentrations in females. However, pharmacokinetic analyses in patients with ADPKD have shown that there are no sex differences in the blood concentration of tolvaptan [20]; therefore, we could not define why female sex was identified as a predictor.
In a previous post hoc analysis of TEMPO 3:4, it was reported that baseline urine osmolality influenced disease progression and the response to tolvaptan in patients with ADPKD [21]. The importance of vasopressin is further supported by recent findings on copeptin levels, a surrogate marker for arginine vasopressin (AVP). Copeptin concentrations are higher in men, positively correlate with TKV growth, and predict response to tolvaptan in ADPKD [22,23]. Subsequent research has confirmed that men have a consistently higher urine osmolality than women [24]. In the present analysis, however, urine osmolality did not predict the effect of tolvaptan in reducing TKV growth. Additionally, there were no differences in urine osmolality at years 1, 2, and 3 between the responder and non-responder groups.
ADPKD risk classification is a practical method for evaluating the prognosis of patients with ADPKD [12,25,26]. In the model developed by Irazabal and colleagues, rates of TKV growth and eGFR decline increase stepwise from class 1A to 1E in ADPKD with a typical presentation. We assessed if there was any difference in risk class distribution between Japanese patients and the global population. In our analysis, classes 1B, 1C, 1D, and 1E constituted 9.5%, 40.8%, 30.6%, and 16.3%, respectively, of the total 147 patients (Table 1), which is a similar distribution to that    [25]. Taken together, our findings indicate no reason to suggest that the effects of tolvaptan in slowing renal function decline are dependent on changes in TKV growth rate. However, the non-responder group included more class 1E patients compared with the responder group (8.1% vs 23.6%). We cannot deny the possibility that TKV growth in the non-responder group might have been much faster and masked the efficacy of tolvaptan.  Several lines of evidence suggest that vasopressin contributes to non-diabetic and diabetic chronic kidney disease progression. Plasma vasopressin levels are increased in animal models and in patients with non-diabetic chronic kidney disease, in animal models of streptozotocin-induced and genetic diabetes mellitus, and in patients with type I and type II diabetes mellitus [27,28]. Plasma levels of copeptin are inversely correlated with GFR [29]. Previous studies have shown that AVP is associated with the progression of chronic kidney disease. Plasma copeptin levels are associated with a decline in kidney function in recipients of kidney transplant [30], and high plasma copeptin levels are associated with lower GFR in patients with type II diabetes mellitus [31]. Furthermore, treatment with AVP V 1a and V 2 receptor antagonists has been shown to cause a significant reduction in blood pressure, proteinuria, and glomerulosclerosis in animal models with 5/6 nephrectomy [32]. Although the underlying mechanisms are unknown, inhibiting the AVP V 2 -receptor may confer a renal protective effect, independent of the kidney volume reduction. Thus, tolvaptan might plausibly be beneficial for patients with ADPKD in whom there is little treatment effect on TKV.
Limitations of this study are its small size and use of a sample from a single ethnic group. Potential differences in genetic features between responders and non-responders warrant further investigation.
Currently, in Japan and other countries, the labeling for tolvaptan in ADPKD stresses its effect on reducing the rate of TKV increase. In the United States, following the Replicating Evidence of Preserved Renal Function: an Investigation of Tolvaptan Safety and Efficacy in ADPKD (REPRISE) trial [33], which did not assess changes in TKV, the Food and Drug Administration approved tolvaptan with an indication to slow renal function decline. In our analysis of the Japanese cohort of TEMPO 3:4, we have shown that tolvaptan suppressed eGFR decline regardless of the effects on TKV growth. Accordingly, we propose that patients receiving treatment with tolvaptan who do not experience a reduction in the rate of TKV growth should not discontinue treatment as there may still be beneficial effects on slowing of renal function decline.

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