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Regulation of Apoptosis and Autophagy During Anoxia in the Freshwater Crayfish, Faxonius virilis

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Abstract

The ability of an animal to survive prolonged periods of oxygen deprivation is a critical area of study, both in terms of its importance to better understanding the physiology of these incredible animals and to its potential applicability to medical fields. The freshwater crayfish, Faxonius virilis, is one such animal capable of resisting anoxia, but it remains understudied and much of the metabolic mechanisms underlying this anoxia tolerance remain largely unprofiled. This study examines the activity and regulation of apoptosis and autophagy in F. virilis in response to 20-h anoxia. Apoptosis signaling was assessed through pro- and anti-apoptosis targets, whereas autophagy was assessed via expression response of multiple autophagy proteins. An anoxia-triggered, tissue-specific result arose, potentially based on the importance of individual organ integrity through hypometabolism. Tail muscle, which showed increased expression profiles of all three target groups, contrasted with hepatopancreas, which appeared to not be susceptible to either apoptotic or autophagic signaling during anoxia. This is likely due to the importance of the hepatopancreas, given that apoptosis or autophagy of this organ at any significant level could be fatal to the organism. The data provides a comprehensive overview of the responses and integration of multiple stress-responsive signaling pathways in F. virilis that provide a novel contribution to our understanding of pro-survival mechanisms supporting invertebrate anoxia resistance.

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Acknowledgements

The authors thank Benjamin Lant for his experimental contributions.

Funding

This work was supported by a Discovery grant (Grant # 6793) from the Natural Sciences and Engineering Research Council (NSERC) of Canada. KBS holds the Canada Research Chair in Molecular Physiology.

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  1. Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON, Canada

    Sarah A. Breedon, Aakriti Gupta & Kenneth B. Storey

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  1. Sarah A. Breedon
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Correspondence to Kenneth B. Storey.

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Breedon, S.A., Gupta, A. & Storey, K.B. Regulation of Apoptosis and Autophagy During Anoxia in the Freshwater Crayfish, Faxonius virilis. Mar Biotechnol 24, 626–639 (2022). https://doi.org/10.1007/s10126-022-10132-0

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