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Repair of UVB-Damaged Skin by the Antioxidant Sulphated Flavone Glycoside Thalassiolin B Isolated from the Marine Plant Thalassia testudinum Banks ex König

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Abstract

Daily topical application of the aqueous ethanolic extract of the marine sea grass, Thalassia testudinum, on mice skin exposed to UVB radiation resulted in a dose-dependent recovery of the skin macroscopic alterations over a 6-day period. Maximal effect (90%) occurred at a dose of 240 μg/cm2, with no additional effects at higher doses. Bioassay-guided fractionation of the plant extract resulted in the isolation of thalassiolin B (1). Topical application of 1 (240 μg/cm2) markedly reduces skin UVB-induced damage. In addition, thalassiolin B scavenged 2,2-diphenyl-2-picrylhydrazyl radical with an EC50 = 100 μg/ml. These results suggest that thalassiolin B is responsible for the skin-regenerating effects of the crude extract of T. testudinum.

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Acknowledgements

The authors are grateful to Boehringer Ingelheim Fonds for financial support provided by a travel grant (E. L. R). We are also grateful to Prof. Raymond J. Andersen (Department of Chemistry, University of British Columbia, Vancouver, Canada) and Dr. Olivier P. Thomas (Department of Marine Natural Products, Faculty of Sciences, University of Nice, France) for collaborating in the results. This work was partially supported by a Grant-in-Aid for Scientific Research No. 038 in 2004–2006 from the Ministry of Science Technology and Environment of Cuba and a research grant (E. L. R) No. 4500045956 of the Intergovernmental Oceanographic Commission of the United Nations Educational, Scientific, and Cultural Organization.

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Authors and Affiliations

  1. Department of Chemistry, Center of Marine Bioproducts, Loma y 37, Alturas del Vedado, C.P. 10400, Havana, Cuba

    Erik L. Regalado, Abilio Laguna, Armando A. Rodríguez, Olga Valdés & Yasnay Hernandez

  2. Department of Chemistry, Laboratory of Cosmetics, Center of Marine Bioproducts, Havana, Cuba

    María Rodríguez & Patricia Lorenzo-Luaces

  3. Department of Pharmacology, Center of Marine Bioproducts, Havana, Cuba

    Roberto Menéndez

  4. Department of Histology, National Center of Medical Genetic, Havana, Cuba

    Ángel A. Concepción

  5. Department of Natural Products, Faculty of Chemistry, University of Havana, Havana, Cuba

    Clara Nogueiras

  6. Department of Chemistry, University of British Columbia, Vancouver, V6T 1Z4, Canada

    David E. Williams

Authors
  1. Erik L. Regalado
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  2. María Rodríguez
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  3. Roberto Menéndez
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  4. Ángel A. Concepción
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  5. Clara Nogueiras
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  6. Abilio Laguna
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  7. Armando A. Rodríguez
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  8. David E. Williams
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  9. Patricia Lorenzo-Luaces
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  10. Olga Valdés
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  11. Yasnay Hernandez
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Corresponding authors

Correspondence to Erik L. Regalado or María Rodríguez.

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Erik L. Regalado and María Rodríguez have contributed equally to this work and should be considered as first authors.

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Regalado, E.L., Rodríguez, M., Menéndez, R. et al. Repair of UVB-Damaged Skin by the Antioxidant Sulphated Flavone Glycoside Thalassiolin B Isolated from the Marine Plant Thalassia testudinum Banks ex König. Mar Biotechnol 11, 74–80 (2009). https://doi.org/10.1007/s10126-008-9123-8

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  • DOI: https://doi.org/10.1007/s10126-008-9123-8

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