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Low-energy LED red light inhibits the NF-κB pathway and promotes hPDLSCs proliferation and osteogenesis in a TNF-α environment in vitro

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Abstract

There are few studies on the effect of low-energy LED red light on periodontal tissue regeneration in an inflammatory environment. In this study, Cell Counting Kit-8 (CCK-8) assays were used to detect the effects of TNF-α at three different concentrations (0, 10 ng/ml, and 20 ng/ml) on the proliferation of human periodontal ligament stem cells (hPDLSCs), and 10 ng/ml was selected as the subsequent experimental stimulation concentration. CCK-8 assays were used to detect the effect of LED red light with energy density of 1 J/ cm2, 3 J/ cm2, and 5 J/cm2 on the proliferation of hPDLSCs. The promotion effect of energy density of 5 J/cm2 on the proliferation of hPDLSCS was the most obvious (p < 0.05). Set CON group, ODM group, ODM + 10 ng/ml TNF-α group, and ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red light group. Alkaline phosphatase staining and activity detection, alizarin red staining and calcium nodules quantitative detection of osteoblast differentiation products, real-time fluorescence quantitative PCR detection of osteoblast gene expression (Runx2, Col-I, OPN, OCN). The results showed that ODM showed the strongest osteoblast ability, followed by ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red light group. The osteoblast ability of ODM + 10 ng/ml TNF-α was decreased, but was not found in CON group. Western blot was used to detect the expression of NF-κB pathway protein and osteoblast-related proteins (Runx2, Col-I, OPN, OCN) after addition of PDTC inhibitor. The results showed that the expression of p-IκBα was increased and the expression of IκBα was decreased (p < 0.05). The expression of osteoblast protein increased after the addition of inhibitor (p < 0.05). Therefore, in an inflammatory environment constructed by 10 ng/ml TNF-α, 5 J/cm2 LED red light can upregulate the proliferation and osteogenesis of hPDLSCs by inhibiting NF-κB signaling pathway.

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Data availability

The data that support the findings of this study are available on request from the corresponding author Yao Wang, upon reasonable request.

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Funding

This work was supported by the Sichuan science and technology plan joint innovation special task project(2022YFS0634); Sichuan Province Medical Research Project (S21015) and University-level scientific research project of Southwest Medical University (2021ZKMS013).

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Author notes

  1. Yuan Liu and Juan Yang contributed equally to this work.

Authors and Affiliations

  1. Institute of Stomatology, Southwest Medical University, Luzhou, 646000, China

    Yuan Liu & Yao Wang

  2. The Third Hospital of Mianyang, Department of Stomatology, Mianyang, 621000, China

    Yuan Liu

  3. Sichuan Mental Health Center, Mianyang, 621000, China

    Yuan Liu

  4. Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou, 646000, China

    Juan Yang, Bing Jiang, Genzi Zheng & Yao Wang

  5. Chenjiaqiao Hospital of Shapingba District Chongqing, Chongqing, 400000, China

    Juan Yang

  6. Dazhou Hospital of Integrated TCM & Western Medicine Hospital, Dazhou, 635000, China

    Bing Jiang

  7. The Third Hospital of Yibin, Department of Stomatology, Yibin, 644000, China

    Genzi Zheng

  8. The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, 646000, China

    Yao Wang

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  1. Yuan Liu
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Correspondence to Yao Wang.

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All procedures performed in the study were in accordance with the Ethics Committee of the Affiliated Hospital of Stomatology Southwest Medical University Certificate (contract grant 20211119003) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Liu, Y., Yang, J., Jiang, B. et al. Low-energy LED red light inhibits the NF-κB pathway and promotes hPDLSCs proliferation and osteogenesis in a TNF-α environment in vitro. Lasers Med Sci 38, 240 (2023). https://doi.org/10.1007/s10103-023-03880-5

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