Antimicrobial activity of ceftibuten-avibactam against a global collection of Enterobacterales from patients with urinary tract infections (2021)

We evaluated the in vitro activity of ceftibuten-avibactam against Enterobacterales causing urinary tract infection (UTI). A total of 3216 isolates (1/patient) were consecutively collected from patients with UTI in 72 hospitals from 25 countries in 2021 then susceptibility tested by CLSI broth microdilution. Ceftibuten-susceptible breakpoints currently published by EUCAST (≤ 1 mg/L) and CLSI (≤ 8 mg/L) were applied to ceftibuten-avibactam for comparison. The most active agents were ceftibuten-avibactam (98.4%/99.6% inhibited at ≤ 1/ ≤ 8 mg/L), ceftazidime-avibactam (99.6% susceptible [S]), amikacin (99.1%S), and meropenem (98.2%S). Ceftibuten-avibactam (MIC50/90, 0.03/0.06 mg/L) was fourfold more potent than ceftazidime-avibactam (MIC50/90, 0.12/0.25 mg/L) based on MIC50/90 values. The most active oral agents were ceftibuten (89.3%S; 79.5% inhibited at ≤ 1 mg/L), levofloxacin (75.4%S), and trimethoprim-sulfamethoxazole (TMP-SMX; 73.4%S). Ceftibuten-avibactam inhibited 97.6% of isolates with an extended-spectrum β-lactamase phenotype, 92.1% of multidrug-resistant isolates, and 73.7% of carbapenem-resistant Enterobacterales (CRE) at ≤ 1 mg/L. The second most active oral agent against CRE was TMP-SMX (24.6%S). Ceftazidime-avibactam was active against 77.2% of CRE isolates. In conclusion, ceftibuten-avibactam was highly active against a large collection of contemporary Enterobacterales isolated from patients with UTI and exhibited a similar spectrum to ceftazidime-avibactam. Ceftibuten-avibactam may represent a valuable option for oral treatment of UTI caused by multidrug-resistant Enterobacterales. Supplementary Information The online version contains supplementary material available at 10.1007/s10096-023-04562-4.


Introduction
Urinary tract infections (UTIs) are among the most common of all bacterial infections. Approximately 50% of all women experience at least 1 UTI by the age of 35, and approximately 20% of women between the ages of 18 and 24 have a UTI annually [1]. The majority of UTIs are treated on an outpatient basis. However, resistance to first-line oral antimicrobials that are used to treat UTIs has increased markedly in the last 2 decades, complicating outpatient treatment approaches [2]. In the USA, Escherichia coli resistant to trimethoprim-sulfamethoxazole (TMP-SMX) among adult females with UTI has exceeded 25%, and among females aged ≥ 65 years, ciprofloxacin resistance approaches 30% [3]. In parts of Europe, resistance to TMP-SMX and ciprofloxacin has also increased dramatically among E. coli [4]. Moreover, side effects of some first-line agents for treatment of UTI are of great concern [5,6].
Other important mechanisms of resistance in UTI pathogens are the production of extended-spectrum β-lactamases (ESBLs) and, more recently, the production of carbapenemases. These resistance mechanisms are frequently associated with fluoroquinolone and/or aminoglycoside resistance determinants [7]. As the number of pathogens resistant to outpatient therapies has risen, the number of hospitalizations for UTIs has also grown [2].
Other oral agents used to treat UTI include fosfomycin and pivmecillinam, the oral version of mecillinam. Most clinical data available is on the effectiveness of fosfomycin to treatment and prevention of lower UTI, primarily cystitis [8]. Although fosfomycin remains very active against E. coli, including MDR and carbapenem-resistant isolates, it has more limited activity against Klebsiella spp. and other Enterobacterales responsible for UTI [9]. Mecillinam is also an old antibiotic that remains very active against E. coil even in regions where it is commonly used to treat UTI; however, a randomized clinical trial comparing this drug with standard of care regimes is warranted [10,11].
Ceftibuten is an oral third-generation cephalosporin which is highly potent against Enterobacterales and stable against many class A and C β-lactamases produced by these organisms, including some ESBLs [12,13]. Avibactam is a synthetic diazabicyclooctane (DBO) non-β-lactam inhibitor. Avibactam is available for clinical use in combination with ceftazidime as an IV formulation; an avibactam formulation for oral use is currently being developed to be combined with ceftibuten for clinical use. Compared with clavulanic acid, sulbactam, and tazobactam, avibactam provides excellent inhibition of most clinically relevant class A and class C β-lactamases, such as ESBLs, KPCs, and AmpC β-lactamases [14]. In the present study, we evaluated the in vitro activity of ceftibuten-avibactam (fixed 4 mg/L) against a contemporary collection of Enterobacterales collected from patients with UTIs worldwide.

Materials and methods
Participant medical centers were invited to collect a specific number (25 to 60, depending on geographic region) of consecutive isolates (1/patient) from patients with UTI in 2021. Only bacterial isolates determined to be significant by local criteria as the reported probable cause of infection were included in this investigation. The organism collection included 3216 isolates from 72 medical centers in 25 countries. Isolates were mainly from the US (n = 1585; 29 centers) and Europe (n = 1410; 33 centers in 18 countries), but also included E. coli isolates from Latin America (n = 121; 6 centers in 5 countries) and Japan (n = 100; 4 centers).
Antimicrobial susceptibility was evaluated by reference broth microdilution method in a monitoring laboratory (JMI Laboratories, North Liberty, Iowa, USA) and conducted according to Clinical and Laboratory Standards Institute (CLSI) procedures (document M07) [15]. Current ceftibuten breakpoints published by CLSI (≤ 8 mg/L) and EUCAST (≤ 1 mg/L) were applied to ceftibuten-avibactam for comparison [16,17]. Avibactam was present at a fixed concentration of 4 mg/L in combination with ceftibuten.

Discussion
Increasing antimicrobial resistance coupled with the lack of new oral antimicrobial agents for MDR organisms represents a major challenge for the treatment of complicated and uncomplicated UTI. Infections caused by ESBL-producing Enterobacterales or CRE usually require IV antimicrobial therapy with very limited options for oral step-down treatment [7,19].
The results of the present study showed that ceftibutenavibactam was very active against a large collection of Enterobacterales isolates causing UTI in various regions of the world. Moreover, ceftibuten-avibactam retained strong activity against isolates with an MDR and/or ESBL phenotype, as well as isolates nonsusceptible to levofloxacin, nitrofurantoin, and/or TMP-SMX. Ceftibuten-avibactam also retained activity against most CRE isolates, inhibiting 73.7% and 78.9% at ≤ 1 mg/L and ≤ 8 mg/L, respectively. Characterization of the isolates with increased ceftibuten-avibactam MIC values (≥ 2 mg/L) is ongoing. Once completed, these characterizations will provide a better understanding of the spectrum of activity for this antimicrobial combination.
The limitations of the study should be considered when interpreting the data. The absence of fosfomycin and pivmecillinam as comparator agents represents a limitation of this investigation since these oral agents are commonly used to treat UTI in some regions. Another limitation of the study is the lack of β-lactamase characterization of ESBL-phenotype and CRE isolates, as well as those with decreased susceptibility to ceftibuten-avibactam. Despite the limitations of the study, the results presented here provide valuable information on the in vitro activity of this novel agent against contemporary isolates from patients with UTI. In summary, these results indicate that ceftibuten-avibactam may represent a valuable addition for the treatment of UTI caused by MDR Enterobacterales. Further pharmacokinetic/pharmacodynamic and clinical studies are warranted to define the role of ceftibuten-avibactam for the treatment of UTI.