Abstract
The flavonoids, wighteone (1), naringenin (2), genistein (3), isoliquiritigenin (4), daidzein (5), daidzin (6), genistein-8-C-β-d-apiofuranosyl-(1→6)-O-β-d-glucopyranoside (7), ambocin (8), and genistin (9) were isolated from roots of Pueraria mirifica. Chemical structures of the compounds were determined based on spectroscopic data analysis. Flavonoids 1, 2, 4, 7, and 8 were isolated for the first time. The contents of 6 flavonoids in P. mirifica roots was determined to be 2.5±0.01 (1), 14.8±0.09 (3), 18.6±0.18 (5), 17.3±0.11 (6), 10.4±0.05 (7), and 6.0±0.02 (9) mg/kg, respectively, using HPLC.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Muangman V, Cherdshewasart W. Clinical trial of the phytoestrogenrich herb, Pueraria mirifica as a crude drug in the treatment of symptoms in menopausal women. Siriraj Hosp. Gaz. 53: 300–309 (2001)
Urasopon N, Hamada Y, Asaoka K, Cherdshewasart W, Malaivijitnond S. Pueraria mirifica, a phytoestrogen-rich herb, prevents bone loss in orchidectomized rats. Maturitas 56: 322–331 (2007)
Kerr A. A reputed rejuvenator. J. Siam Soc. Nat. History 8: 336–338 (1932)
Sukawattana T. Oestrogenic principle of Butea superba, preliminary report. J. Med. Assoc. Thailand 24: 183–194 (1940)
Jaroenporn S, Malaivijitnond S, Wattanasiirmkit K, Trisomboon H, Watanabe G, Taya K, Cherdshewasart W. Effects of Pueraria mirifica, an herb containing phytoestrogens, on reproductive organs and fertility of adult male mice. Endocr. J. 30: 93–101 (2006)
Jaroenporn S, Malaivijitnond S, Wattanasirmkit K, Watanabe G, Taya K, Cherdshewasart W. Assessment of fertility and reproductive toxicity in adult female mice after long-term exposure to Pueraria mirifica herb. J. Reprod. Dev. 53: 995–1005 (2007)
Ministry of Food and Drug Safety. Available from: https://www.mfds.go.kr. Accessed Dec. 31, 2013.
Chansakaow S, Ishikawa T, Seki H, Sekine K, Okada M, Chaichantipyuth C. Identification of deoxymiroestrol as the actual rejuvenating principle of “Kwao Keur”, Pueraria mirifica. The known miroestrol may be an artifact. J. Nat. Prod. 63: 173–175 (2000)
Chansakaow S, Ishikawa T, Sekine K, Okada M, Higuchi Y, Kudo M, Chaichantipyuth C. Isoflavonoids from Pueraria mirifica and their estrogenic activity. Planta Med. 66: 572–575 (2000)
Lee JH, Lee BW, Kim B, Kim HT, Ko JM, Baek IY, Seo WT, Kang YM, Cho KM. Changes in phenolic compounds (isoflavones and phenolic acids) and antioxidant properties in high-protein soybean (Glycine max L., cv. Saedanbaek) for different roasting conditions. J. Korean Soc. Appl. Biol. Chem. 56: 605–612 (2013)
Kinoshita T, Ichinose K, Takahashi C, Ho FC, Wu JB, Sankawa U. Chemical studies on Sophora tomentosa: The isolation of a new class of isoflavonoid. Chem. Pharm. Bull. 38: 2756–2759 (1990)
Jeon SH, Chun W, Choi YJ, Kwon YS. Cytotoxic constituents from the bark of Salix hulteni. Arch. Pharm. Res. 31: 978–982 (2008)
Kinjo JE, Furusawa JI, Baba J, Takeshita T, Yamasaki M, Nohara T. Studies on the constituents of Pueraria lobata. III. Isoflavonoids and related compounds in the roots and the voluble stems. Chem. Pharm. Bull. 35: 4846–4850 (1987)
Ma WG, Fukushi Y, Hostettmann K, Tahara S. Isoflavonoid glycosides from Eriosema tuberosum. Phytochemistry 49: 251–254 (1998)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cho, JG., Park, HJ., Huh, GW. et al. Flavonoids from Pueraria mirifica roots and quantitative analysis using HPLC. Food Sci Biotechnol 23, 1815–1820 (2014). https://doi.org/10.1007/s10068-014-0248-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10068-014-0248-4