A simplified methodology: pH sensing using an in situ fabricated Ir electrode under neutral conditions

Herein, a simplified fabrication method for the producing of a pH-sensitive iridium electrode is developed. The in situ electrochemical fabrication of an iridium oxide film is optimized and shown to be achievable under neutral conditions rather than the acidic conditions hitherto employed. The formation of a pH sensitive Ir(III/IV) hydrous film is confirmed via XPS. The amperometric pH-sensing properties of this electrochemically generated material were investigated using square wave voltammetry. In the pH range 2–13, the iridium oxide redox signal has a pH dependency of 86.1 ± 1.1 mV per pH unit for midpoint potentials with uncertainties being ± 0.01–0.05 pH. Finally, the newly developed pH sensor was used to measure the pH of a natural water sample with excellent results as compared to a conventional glass pH probe.


Introduction
The determination of pH quantifies how acidic or basic an aqueous solution is. It is probably the most commonly made measurement by a chemical sensor. Real-world examples of the importance of pH measurements abound. For example, the human body can be viewed as a large buffer solution with the blood pH being maintained within the near neutral range. Abnormally high blood pH (alkalosis), or abnormally low blood pH (acidosis) is used as an indicator of numerous health issues including Bartter (BS) and Gitelman (GS) syndromes and hyperlactatemia [1][2][3]. Alternatively in environmental applications, pH is directly linked to the solubility of heavy metals; the lower pH the more toxic the water possibly is as the metals tend to be more soluble [4]. In food chemistry, pH data reveals the fractionation, solubility and functional properties of essential nutrients [5].
IUPAC [6] defines pH as a function of the activity of the hydrogen ion in a solution: where a H + is the single ion activity (measured on the molality scale in mol kg −1 ), γ H + is the activity coefficient of the hydrogen ion (H + ) at a molality of m H + and m θ is the standard molality (1 mol kg −1 ) [7]. Note that IUPAC chose to define the pH in terms of the single ion quantities despite it often being asserted that these are impossible to measure other than approximately [8,9], although some ingenious work arounds have been proposed [10,11]. In contrast, Rockwood has proposed an approach based on classical equilibrium thermodynamics and provides a clear historical summary of the debate [12].
To date, various methods, electrochemical and nonelectrochemical, have been applied to measure pH values, summarised by Vonau and Guth [13]. The glass pH metre is ubiquitously used in the laboratory by applying direct potentiometric measurements. It is a rapid and convenient method for determining the activity of the hydrogen ion in a solution. However, there are errors affecting its use, most notably the alkaline/acid errors at too high or too low pH, dehydration of the electrode and a reliance on the accuracy of standard buffers [14]. Moreover, there is an essential need for regular, frequent and careful calibration, which must be referenced to a certified buffer solution. Note that the pH value and uncertainty of the reference buffer solution should also be determined using a calibrated measurement. Thus, a traceability chain can be established relating a measured pH to the pH of a primary buffer solution, the basis and the historical development of which is described by Spitzer [15]. Alternatives to the glass electrode for potentiometric sensing have been suggested, for example by Inzelt [16,17]. More generally, the use of potentiometric measurements however make precise and sensitive measurements challenging since the logarithmic relation between potential and activity results in a change of signal of a mere ca 60 mV per decade of concentration at 298 K. Apart from a glass electrode, electrode materials like carbon can be functionalised for pH sensing by various surface modifications, e.g. film formation and physical adsorption [18]. Electrodes can also be chemically modified with organic compounds or polymers, such as quinhydrone [19][20][21][22][23] and polypyrrole [24]. These provide an amperometric response which can be sensitive and selective to H + ions [19,[24][25][26][27]. One advantage of using amperometrically measured peak potentials is the scope for the doubling of sensitivity to 120 mV/decade through the use of two simultaneously immobilised pH-sensitive redox couples [28,29]. A further merit of amperometric measurements is the ability to make calibration free pH measurements [30], while on the other hand, they are restricted to media containing some electrolyte. Outside of electrochemistry, the fibre optic pH sensor is a non-electrochemical sensor with low cost and the ability for continuous sensing, which is commonly used in physiological studies [31][32][33][34]. Optical pH sensors usually consist of a pH indicator immobilised on an organic carrier. The indicator changes its chemical structure after interacting with H + ions, which leads to a variation of its optical response as a function of pH [35]. However, because the response of the colour change is sigmodal with pH, the accuracy is constrained and the useful detection range can be relatively narrow, often being 2-4 pH units only [36]. To be more specific, the optical pH sensors can be based on absorption [33], reflectance intensity [37] and fluorescence intensity [34,38,39]. The use of fluorescence is common due to its high sensitivity compared with reflectometric devices [39]. The short response time and scope for miniaturisation are further advantages. Notably, Optiz and Lubbers developed a fluorescence pH sensor that can measure the ionic strength and hydrogen ion activities simultaneously and continuously using hydroxypyrenetrisulfonic acid (HPTS) and β-methylumbelliferone [40]. Other indicators include 5-(and 6-) carboxynaphthofluorescein [39], and 7-hydroxycoumarin-3-carboxylic acid (HCC) [38] for pH measurements near-neutral values.
Metal/metal oxides or metal oxides pH sensors are widely studied especially in potentiometric mode based on the Nernst equation [41]. The potential determining reactions of some transition metal oxides MO x involves H + ions and the number of electrons transferred during the process and its specific mechanism decide the E -pH relationship. pH sensitive metal/metal oxide systems M/MOx have been investigated including examples where M is antimony (Eqs. 2-3) [42][43][44][45][46][47][48], palladium (Eqs. [4][5] [43,44,49,50], ruthenium (Eq. 6) [51][52][53] and tungsten (Eqs. 7-8) [54][55][56] and applied as pH sensors. The following are typical of the potential determining equilibria for typical M/MO x systems [54,55]: While metal/metal oxide systems are commonly used in classical potentiometric mode in combination with a reference electrode to generate a potential reflecting the pH [44,[57][58][59][60], it is also possible to employ them in amperometric mode in which a pH sensitive voltammetric signal is used, the peak potential of which is used to indicate the pH [61][62][63]. One attraction of this approach is that the measurement generates a current-voltage plot the shape of which allows the assessment of the 'quality' of the measurement and hence the reliability of the data. This is particularly important under conditions where electrode fouling may occur, as in blood or other in vivo analysis [26,64,65], where in contrast to potentiometric measurements which simply deliver a number, amperometry/voltammetry allows the peak shape to provide an indication of the extent of for example electrode fouling or flag up a mis-functioning electrode [66]. One system where both potentiometry [44,57,[67][68][69][70] and amperometry [26,64] has been used in this way is the iridium/iridium oxide system.
The Ir/IrO x redox couple is one of the most popular candidates for pH sensors especially in aggressive environments such as high temperature and/or pressure [57,67,69,[71][72][73][74], which is also famous for its electrocatalytic behaviour for the O 2 and Cl 2 evolution reactions [75][76][77][78][79] and electrochromic properties of redox reactions [67,[71][72][73]. There are diverse methods for Ir oxide generation. Electrodeposition is one of the methods and uses a solution of potassium hexachloroiridate(III) as a precursor [64,80,81]. The conditions and process required to form the final IrO x are complex and hard to control, which lead to limited reproducibility [82][83][84][85]. Iridium oxides can also be prepared by sol-gel [86,87] chemistry, sputtering [59,88] or thermal methods [69,85]. However, a pre-treatment, e.g. hydroxylation, is required prior to the usage of electrodes fabricated by the latter two approaches. This is because the resultant dry oxide films or anhydrous films are less hydrous than iridium oxide prepared electrochemically in an aqueous solution [64,68,89]. Meanwhile, sputtering is expensive, whereas the pH response of the thermally synthesised Ir electrode is highly dependent on the fabrication conditions [90].
It has been reported that the anodic iridium oxide film (AIROF) can be synthesised by cyclic voltammetry and the potential determining redox reaction happens within the iridium oxide film rather than on the Ir metal which simply serves as a conductive support [78,79,[91][92][93]. Similar phenomena were also observed for Rh, Ru, Os and W [75,[94][95][96][97]. Based on this, in this paper, we develop a novel in situ AIROF fabrication methodology for pH sensing. Instead of employing ex situ potential cycling under acidic or basic conditions as previously explored [43,67,92,93,98,99], neutral conditions were newly applied with AIROF being fabricated in situ so facilitating more remote and diverse pH measurements notably in medical and environmental applications. To further simplify the procedure, constant potential electrolysis (CPE) activation was applied and further optimised to mimic and replace CV activation, followed by square wave voltammetry (SWV) to obtain resultant the redox peaks used for pH measurement. The pH dependency of the redox peak position and scope of the method are studied in buffer solutions with different pH values. The methodology is applied to the pH measurement in authentic samples of pond water giving good agreement with pH values obtained independently by a classical commercial pH metre.

Electrochemical apparatus and methods
A high purity Ar flow was used to remove oxygen from all aqueous solutions prior to study. Electrochemical measurements were performed in a thermostatic (25 ± 1 °C) Faraday cage using a CHI760E Electrochemical Workstation (CH Instruments, Inc., Shanghai, China). A standard three electrode set up was used, consisting of a mercury/mercury sulphate/saturated K 2 SO 4 solution reference electrode (MSE, + 0.64 V vs NHE at 25 °C, CH Instruments, Inc., China), a platinum wire counter electrode and an iridium electrode (0.1 mm in diameter, GoodFellow, UK) as the working electrode. An Ir electrode was pretreated by heating the metal using a flame gun using gas liquefied petroleum gas (butane ≥ 95%, Pan'an city Gas Co., Ltd., China) for 10 s to remove surface contamination and impurities. Cyclic voltammetry was used to study the electrochemical behaviour of the Ir electrode, while square wave voltammetry was conducted to determine the pH dependency of in situ modified Ir wire after constant potential electrolysis (CPE) activation.

XPS study and sample preparation
The four pure Ir wires (0.125 mm in diameter, Alfa Aesar (China) Chemicals Co., Ltd) were preflamed as discussed above. For three preflamed Ir wires, the potential cycling activation, starting at − 0.6 V vs MSE then scanning anodically to 0.45 V vs MSE and then cathodically to − 1.05 V vs MSE for 625 cycles was conducted in a degassed PBS solution (pH = 7.25). Then, the cyclic voltammetry was stopped at − 1.05 V, − 0.1 V or 0.3 V vs MSE, respectively, followed by application of the end potential for 60 s. The three Ir samples with electrochemical activation and the one without were sealed in separate vacuum tubes. The calibrated X-ray photoelectron spectroscopy (XPS, K-Alpha +) spectra are referenced to the C 1 s peak at 284.6 eV and were carried out for surface elemental analysis.

Real sample analysis
Pond water from Wenshan Lake located in Shenzhen University, China (113.93° E, 22.54° N), was used for the real sample analysis. Prior to further analysis, natural water samples were filtered using filter paper (pore size 1 μm) to remove solid impurities. The KCl was then added to the natural water to realise a concentration of 100 mM.

Results and discussion
In the following sections, we first explore the oxidative voltammetry of iridium metal under conditions of near neutral pH to show the in situ formation of iridium oxide features which are subsequently used as the basis for amperometric pH sensing. XPS is used to confirm the inferred redox chemistry. Next, the in situ formation of iridium oxide under conditions of different fixed potentials is explored with the aim of permitting in situ sensor generation from an iridium electrode. This process is then optimised for the formation of pH sensitive iridium hydrous oxides. The pH response was further explored using square wave voltammetry (SWV) and an analytical methodology for pH defined in which the surface bound pH sensing redox couple is generated in situ from an iridium wire. Finally, the developed method was employed to determine the pH of a natural water sample.

Cyclic voltammetric study of Ir metal electro-oxidation
The formation of iridium oxides on the surface of an iridium wire was investigated voltammetrically. It was recognised that for reproducible data prior to a measurement, the surface of the Ir wire should be completely free of oxide which is commonly found on such surfaces. For some electrode materials, it is possible to remove such traces of oxide electrochemically but this is known to be unreliable for iridium, leaving a small amount of hydrous oxide on the surface [92,100,101]. For this reason, prior to voltammetry, Ir surfaces were treated by pre-flaming in the present work to renew the surface of the metal electrode between each experiment. The effects of pre-flaming are shown in Sect. 1 of SI where a clear memory effect for an electrode without preflaming was observed, whereas the cyclic voltammograms are reproducible when using a preflamed electrode.
To explore the electrochemical behaviour of an Ir wire in neutral solution, the pre-flamed Ir electrode was immersed in a degassed phosphate buffer solution (PBS) with 0.1 M KCl (pH = 7.25) and cyclic voltammetry was conducted for 625 cycles starting at a potential of − 0.6 V with scan reversal at a potential of 0.45 to − 1.05 V vs MSE where the potential again reversed as shown in Fig. 1a. The figure shows four voltammetric features labelled A, B, C and D. Feature A is assigned to the underpotential adsorption of hydrogen (H upd ) [102,103].
In contrast to feature A, features B and C steadily increase with increasing scan number. This voltammetric response is markedly different from that observed for platinum [104]. Here, feature B is attributed to the Ir (III)-Ir (IV) redox transition with formation of hydrous oxides. This process has been reported to involve various possible redox reactions between the reduced and oxidised forms of oxyhydroxide where the exact stoichiometric composition of the hydrous film is reported as hard to determine [64, The blue lines show the potentials used for constant potential oxide generation (see text). (b) Schematic mechanism of Ir electrode activation. BHO, bulk hydrous oxide; HSL, hydrated surface layer [92] 67, 105]. The equilibria suggested by Olthuis et al. [67] and Burke et al. [105] are as follows: The equilibria in (10) and (11) suggest a super-Nernstian pH dependency of ca 89 mV per pH unit, reflecting the fact the relative numbers of electrons and protons consumed in reaction (10) and (11) [57,64,106].
There are different interpretations of feature C. Pickup and Birss suggested that it corresponds to further oxidation of the Ir hydrous oxides, e.g. from Ir (IV) to Ir (VI) [92]. However, later reports from Pfeifer et al. [78,79,91] proposed assigning it to the oxidation of the oxide anion O 2− , contained in the IrO x matrix in form of adsorbed OH groups, to O − : Feature D is related to the oxygen evolution reaction (OER): It has been suggested that the IrO x O I− formed during the redox reaction in feature C is the catalyst for OER where Eq. (14) explains its possible electrocatalytic behaviour explicitly [79]: Based on the literature, it is possible to summarise the likely sequence of events underpinning the voltammetry shown in Fig. 1. Initially during the first sweep of potential cycling, the pure Ir is oxidised to IrO 2 by potentials ranging from − 0.65 to 0.15 V vs MSE and its surface immediately becomes hydrated when an anodic potential is applied. The resulting Ir (IV) hydrous oxide can subsequently only be reduced back to Ir (III) in contrast to IrO 2 with the applied potential negative than approximate − 1.05 V which is thought to be capable of being reduced to pure Ir when the cyclic voltammetry is swept cathodically [92]. Figure 1b schematically shows the state of the surface of the iridium electrode at different stages of the voltammetry and seeks to explain the build-up of the features B and C from one potential cycle to the next. In Fig. 1b, phase 1 shows a pure Ir wire with bulk Ir(III) hydrous oxide (BHO Ir(III)) accumulated from previous scans on its surface. When an anodic potential is applied (10) (between − 0.65 and 0.15 V vs MSE), the Ir metal is again partly oxidised to IrO 2 and the previously formed BHO Ir(III) is oxidised to form BHO Ir(IV). Note that the Ir (IV) hydrated surface layer (HSL Ir (IV)) shown at phase 2 differs from BHO Ir(IV) formed in previous cycles in that it is newly formed and bonded to the inner IrO 2 layer. Then, by applying a cathodic potential at approximately − 1.05 V vs MSE, neither the BHO nor the HSL Ir (IV) can be reduced to oxidation states lower than Ir (III). As a result, a new layer of BHO Ir (III) from HSL Ir (IV) is added to the original Ir metal surface as shown in phase 3. Consequently, as the number of potential cycling increases as shown in Fig. 1, a progressive build-up of the Ir hydrous oxide layer was observed with scans due to the repeated redox process mentioned above. Referring to the cyclic voltammogram in Fig. 1a, the thickness of accumulated Ir hydrous oxide after 625 cycles is estimated to be around 0.46 μm, assuming that the H upd represents a monolayer (Sect. 2 of SI).
To confirm the proposed interpretation and analyse the formed Ir hydrous oxide layer, three surfaces were electrochemically generated for XPS with a pure Ir wire surface cleaned by flaming for comparison. Figure SI Table 1 states the details of the spectra, which are consistent with the proposed mechanism above and to be more specific, the formed thick layer is iridium (III/IV) hydrous oxide as a result of potential cycling. The presence of this even when the sample for spectroscopic evaluation being withdrawn at − 1.05 V indicates the chemical irreversibility of the formation of this layer (see detailed discussion in Sect. 3 of SI).
In a conclusion, an Ir wire can be activated under neutral rather than acid conditions forming a hydrous iridium (III/ IV) oxide layer, which is pH-sensitive. In the following, we use this approach to develop a pH sensor based on the in situ activation of an Ir electrode under neutral pH conditions.

Optimisation of the electrode activation
In this sub-section, the most effective potential window of potential cycling for the growth of Ir hydrous oxide was first studied based on the conclusions of the last section. Next constant potential oxidation was optimised to mimic the cyclic voltammetry by applying various fixed potentials for defined periods of time to develop a method of in situ generation of the pH sensitive Ir hydrous oxide. Square wave voltammetry (SWV) was then conducted to record the redox peaks of the resulting modified Ir surfaces under different pH conditions and an analytical methodology developed.

Optimisation of the potential window for the growth of pH-sensitive iridium hydrous oxide
The formation mechanism of the Ir hydrous oxide layers was clarified in the previous section. Next, the potential window of cycling was optimised to improve the sensitivity in respect of pH detection. As shown in Fig. 2a, cyclic voltammetry of a pre-flamed Ir wire with different potential windows were conducted in a degassed PBS solution with 0.1 M KCl (pH = 7.25) at 0.5 V s −1 . The CV starts at a potential of − 0.6 V and first swept anodically to a fixed potential of 0.45 V and the swept to different cathodic limits varies from − 1.05 to − 0.7 V vs MSE. The 10th cycle is displayed in Fig. 2a to find the optimised cathodic potential. Figure 2b shows the 10th cyclic voltammograms with the cathodic potential being fixed at − 1.05 V and the anodic potential was progressively increased to 0.5 V from 0.2 V vs MSE.
The resultant Ir (III/IV) redox couple in Fig. 2a becomes better defined with a high signal to background level with a reductive limit of − 1.05 V while in Fig. 2b, the redox couple of the 10th cycle becomes better-defined when the positive potential limit is extended to 0.45 V. Extending the limits beyond − 1.05 V or + 0.45 V showed no further improvement in the quality of the signal, which is consistent with literature [92,107]. Pickup and Birss also suggested that the hydrous oxide begins to dissolve when more positive potentials were applied [92]. From our measurement, it is clear that the potentials between 0.2 and 0.45 V has greater influence on the formation of the Ir hydrous oxide, while extension of the cathodic limit increases the rate of growth only slightly. An explanation for this may be that the completion of redox transition between O 2−/− contained in the IrO x matrix in form of adsorbed OH groups (feature C) is a prerequisite of hydrous oxide formation.
To summarise, for the growth of hydrous oxide, the potential window must embrace both the upper critical limit and the lower critical limit, to be more specific, anodically to 0.45 V and cathodically to − 1.05 V vs MSE. One can also conclude that during cyclic voltammetry, only around 20% of the experimental potential scan while the electrode is at extreme potentials actually induces the chemistry for the growth of hydrous oxide. This latter observation encourages seeking a simpler activation method using constant potential electrolysis as below.

Optimisation of constant potential electrolysis for in situ Ir hydrous oxide growth
Different potential sequences were developed with timescales dictated by the observation that potential cycling between − 1.05 and 0.45 V for 10 cycles was effective to obtain a clear redox couple suggesting that around 12 s might be needed at each potential limit to demonstrate a comparable effect as the potential cycling. Thus, three CPE methods were analysed. Figure 3a-c are the plots of potentials against time to demonstrate the three CPE activations to mimic and enhance the potential cycling activation established as above. For method 1 in Fig. 3a, a potential of 0.45 V was applied for 12 s and followed by a potential of − 1.05 V for 12 s. Method 2 has the same total activation Fig. 2 Cyclic voltammograms of the 10th cycle in degassed buffer solution with pH = 7.25 starting at a potential of − 0.6 V for activation of Ir wire with different (a) cathodic and (b) anodic potentials at a scan rate of 0.5 Vs −1 time at each limit potential but was divided into six repeats of potential so that 2 s was spent on each potential on every repeat (Fig. 3b). As shown in Fig. 3c, method 3 is a CPE method with four repeats of potential switch (2 s for each potential segment) for a shorter overall duration (16 s rather than 24 s). Figure 3d shows the three cyclic voltammograms resulting from the three activation methods and, as expected, display the presence of two redox couples (Ir 3+/4+ and O 2−/− ) as characterised above with formal potentials of ca. − 0.26 V and 0.13 V vs MSE, respectively. Comparison of the three voltammograms shows that method 1 produces a much weaker signal than methods 2 and 3 but that there is a negligible difference between the later.
We conclude that CPE is effective for the growth of Ir hydrous oxide films and that method 3 is suitable. Hence, CPE with four repeats of potential switch between 0.45 and − 1.05 V vs MSE will be used as the in situ Ir electrode fabrication method for further study as reported below.

pH dependency of the iridium hydrous oxide layer in buffer solutions
In this section, the pH dependency of the iridium hydrous oxide layer is investigated by square wave voltammetry (SWV) using an Ir wire activated by the CPE method established as above. The optimisation of SWV parameters, including frequency, step potential and amplitude, in a degassed PBS solution with 0.1 M KCl (pH = 7.25) was implemented to obtain the best-defined square wave voltammograms for pH measurements (Sect. 4 of SI). The optimised SWV with frequency of 90 Hz, amplitude of 60 mV and step potential of 1 mV were applied for pH measurements in buffer solutions with various pHs. Prior to the measurements, the pHs of buffers were measured by a pH metre, with an uncertainty of 0.01 (x-axis error-bar in Fig. 4c). SWV were first scanned anodically to obtain a net oxidative peak and then scans were immediately reversed to obtain a desired reduction peak ( Fig. 4a and b). The expected Ir(III/IV) SWV peaks were observed in the potential range within ca 0.11 to − 0.73 V for scans in both directions for pH = 2-13, while for pH = 2-5, a small peak was seen during the oxidation scan, with peak potentials being about 0.32 V more negative than that of the target oxidation peak. But the pre-peak was deemed not to interfere with the measurements of the main peak to be used for the pH determination. For pH values lower than 2 (Sect. 5 of SI), three peaks were observed during oxidation scans, notably, at ca − 0.12 V, 0.09 V and 0.31 V for pH = 1.15 and − 0.11 V, 0.06 V and 0.22 V vs MSE for pH = 1.86 (Fig. S5a). The reduction signals were broad for both pH values, and this was attributed to the overlap of 2 peaks (see Fig. S5b). Given the voltammetric complexity seen at low pH, it was decided to focus on the pH range from 2.0 to 13.0 for establishing a measurement protocol.
The analytically useful (for pH measurement) Ir(III/ IV) redox couple obtained in solutions with pH = 2-13 showed a shift in the peak potentials towards more negative potentials as the pH increased for both oxidative and reductive scans. To analyse the data, the oxidation/reduction peak potentials and midpoint potential of the in situ fabricated iridium oxide electrode were all recorded. The plots of potentials against pH as measured from pH metre are shown in Fig. 4c. The gradients of the slopes were 85.6 ± 2.3, 90.0 ± 2.1 and 86.1 ± 1.1 mV per pH unit for oxidation/reduction peak potentials and midpoint potential, respectively. The pH dependency is above the ca 60 mV per pH unit response of a classical glass electrode but consistent with the equilibria proposed by Olthuis et al. [67] and Burke et al. [105] being around 89 mV per pH unit as given in Eqs. 9 and 10 above where three protons are transferred per two electrons. The higher response per pH unit is a clear merit of the iridium oxide approach to pH sensing.
For the uncertainty of pH measurement, bootstrap was used [108]. Each of these simulated pH values by bootstrap has its own values of mean and uncertainty (Table 2, Sect. 6 of SI). Oxidation peaks and midpoints have standard deviations smaller that of reduction peaks (0.2 to 0.7), while midpoints are considered to be the reference for the later application, with pH uncertainties ranging from 0.1 to 0.5.

Real sample analysis: pH measurement in natural water
The pH dependency of the in situ fabricated iridium hydrous oxide layer on the iridium wire in buffer solutions was obtained and investigated in the previous section using CPE activation method followed by SWV to improve signal-tobackground ratio of the redox peak of interest. This electrode was subsequently employed for pH sensing of a natural water sample (Wenshan Lake located in Shenzhen University, China) to investigate the capability of the use under real world conditions. First, the iridium wire was activated using CPE with 4 repeats of potentials which switched between 0.45 and − 1.05 V vs MSE in a PBS solution with 0.1 M KCl (pH = 6.91). Then, SWV with the step potential of 1 mV, amplitude of 60 mV and frequency of 90 Hz was conducted in the pond water containing 0.1 M KCl. The effect of 0.1 M KCl as supporting electrolytes in pond water on the pH measurement using an Ir wire is demonstrated in Sect. 7 of SI. The oxidation peaks were recorded using SWV which initially swept to positive potentials and the scans were then reversed to obtain reduction peaks (blue dashed lines in Fig. 5a and b). The redox peaks obtained in the natural water samples were overlaid with previous results (solid lines). Three repeat experiments were done for an improved accuracy of the measurements; the average Ir(III/IV) oxidation peak from SWV is at − 0.195 ± 0.005 V and reduction peak is at − 0.241 ± 0.007 V with a corresponding average mid-point potential of − 0.218 ± 0.005 V vs MSE. The pH value of the pond water sample was then calculated to be 6.92 ± 0.08 by adapting the pH dependency obtained in buffer solutions (blue dots in Fig. 5c). The reliability and accuracy for pH sensing in pond water using such iridium electrode have been proved by comparing the calculated pH value (6.92 ± 0.08) and the value measured by a commercial pH metre (6.91 ± 0.01). Thus, one can conclude that this in situ fabricated Ir wire is an ideal pH sensor under neutral condition.

Conclusions
In this work, the fabrication and performance of an iridium oxide electrode was studied. First, the cyclic voltammetry combined with XPS study demonstrated that an Ir wire can be activated under neutral rather than acid or basic conditions and the pH sensitivity of the formed hydrous iridium oxide layer is controlled by the Ir (III/IV) couple at all potentials used for pH measurement. Cyclic voltammetry was used to identify the potentials required to form the pH sensitive layers allowing a significant simplification of the formation by using constant potential electrolysis (CPE) with four repeats of potential switch between 0.45 and − 1.05 V vs MSE rather than potential cycling. Then, the pH dependency of the electrode was studied in buffer solutions employing square wave voltammetry. The detection limit is pH = 2-13 and the gradients of the slopes being 85.6 ± 2.3, 90.0 ± 2.1 and 86.1 ± 1.1 mV per pH unit for oxidation/reduction peak potentials and midpoint potentials, respectively. Uncertainties of pH measurement are ± 0.01-0.05 pH units for midpoint potential. Finally, the measured pH of a natural water sample using the Ir electrode is 6.92 ± 0.08, while that obtained by a pH metre is 6.91 ± 0.01. The insignificant difference proves that this in situ fabricated Ir wire is an ideal pH sensor for use under neutral conditions and where in situ generation is advantageous.
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