Abstract
The isolated halophilic bacterial strain Halovibrio variabilis TG-5 showed a good performance in the pretreatment of coal gasification wastewater. With the optimum culture conditions of pH = 7, a temperature of 46 °C, and a salinity of 15%, the chemical oxygen demand and volatile phenol content of pretreated wastewater were decreased to 1721 mg/L and 94 mg/L, respectively. The removal rates of chemical oxygen demand and volatile phenol were over 90% and 70%, respectively. At the optimum salinity conditions of 15%, the total yield of intracellular compatible solutes and the extracellular transient released yield under hypotonic conditions were increased to 6.88 g/L and 3.45 g/L, respectively. The essential compatible solutes such as l-lysine, l-valine, and betaine were important in flocculation mechanism in wastewater pretreatment. This study provided a new method for pretreating coal gasification wastewater by halophilic microorganisms, and revealed the crucial roles of compatible solutes in the flocculation process.
Similar content being viewed by others
Data availability
All experimental data are available upon request.
Abbreviations
- CGW:
-
Coal gasification wastewater
- NH4 +–N:
-
Ammonia nitrogen
- COD:
-
Chemical oxygen demand
- TSS:
-
Total suspended solids
- PCR:
-
Polymerase chain reaction
- ML:
-
Maximum Likelihood
- LC–MS:
-
Liquid chromatography–electrospray mass spectrometry
References
Abdelhafez OH, Othman EM, Fahim JR, Desoukey SY, Pimentel-Elardo SM, Nodwell JR, Schirmeister T, Tawfike A, Abdelmohsen UR (2019) Metabolomics analysis and biological investigation of three malvaceae plants. Phytochem Analy 31(2):204–214
Aljuboori AH, Idris A, Al-Joubory HH, Uemura Y, Ibn Abubakar BS (2015) Flocculation behavior and mechanism of bioflocculant produced by Aspergillus flavus. J Environ Manag 150:466–471
Aston JE, Peyton BM (2007) Response of Halomonas campisalis to saline stress: changes in growth kinetics, compatible solute production and membrane phospholipid fatty acid composition. FEMS Microbiol Lett 274(2):196–203
Banciu HL, Gridan IM, Zety AV, Baricz A (2022) Asgard archaea in saline environments. Extremophiles 26(2):21–28
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Demurtas A, Pescina S, Nicoli S, Santi P, Ribeiro de Araujo D, Padula C (2020) Validation of a HPLC-UV method for the quantification of budesonide in skin layers. J Chromatogr B 1164:122512
Fan CZ, Lu AH, Li Y, Wang CQ (2010) Pretreatment of actual high-strength phenolic wastewater by manganese oxide method. Chem Eng J 160(1):20–26
Gai HJ, Jiang YB, Qian Y, Kraslawski A (2008) Conceptual design and retrofitting of the coal-gasification wastewater treatment process. Chem Eng J 138(1–3):84–94
Gai HJ, Feng YR, Lin KQ, Guo K, Xiao M, Song HB, Chen XL, Zhou H (2017) Heat integration of phenols and ammonia recovery process for the treatment of coal gasification wastewater. Chem Eng J 327:1093–1101
George WH, Nishi V, Albert B (2005) Characterisation of a highly stable α-amylase from the halophilic archaeon Haloarcula hispanica. Extremophiles 9(6):487–495
Hou BL, Han HJ, Jia SY, Zhuang HF, Zhao Q, Xu P (2014) Effect of alkalinity on nitrite accumulation in treatment of coal chemical industry wastewater using moving bed biofilm reactor. J Environ Sci 26(5):1014–1022
Hu X, Chen K, Lai XK, Yao Q, Ji SP, Kaiser K (2016) Treatment of pretreated coal gasification wastewater (CGW) by magnetic polyacrylic anion exchange resin. J Environ Chem Eng 4(2):2040–2044
Imhoff JF, Rahn T, Künzel S, Keller A, Neulinger SC (2020) Osmotic adaptation and compatible solute biosynthesis of phototrophic bacteria as revealed from genome analyses. Microorganisms 9(1):46
Ji QH, Tabassum S, Yu GX, Chu CF, Zhang ZJ (2015a) Determination of biological removal of recalcitrant organic contaminants in coal gasification waste water. Environ Technol 36(22):2815–2824
Ji QH, Tabassum S, Yu GX, Chu CF, Zhang ZJ (2015b) A high efficiency biological system for treatment of coal gasification wastewater—a key in-depth technological research. RSC Adv 5(50):40402–40413
Ji QH, Tabassum S, Hena S, Silva CG, Yu GX, Zhang ZJ (2016) A review on the coal gasification wastewater treatment technologies: past, present and future outlook. J Clean Prod 126:38–55
Kim M, Oh H-S, Park S-C, Chun J (2014) Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. IJSEM 64(Pt_2):346–351
Kuhlmann AU, Bremer E (2002) Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp. Appl Environ Microbiol 68(2):772–783
Le BS, Paniagua D, Vazquez-Duhalt R (2008) Biodegradation of organic pollutants by halophilic bacteria and Archaea. J Mol Microb Biotech 15(2–3):74–92
Lewis M, Pryor R, Wilking L (2011) Fate and effects of anthropogenic chemicals in mangrove ecosystems: a review. Environ Pollut 159(10):2328–2346
Li HQ, Han HJ, Du MA, Wang W (2011) Removal of phenols, thiocyanate and ammonium from coal gasification wastewater using moving bed biofilm reactor. Bioresour Technol 102(7):4667–4673
Li C, Zhang CY, Song GL, Liu H, Sheng GH, Ding ZF, Wang ZL, Sun Y, Xu Y, Chen J (2015a) Characterization of a protocatechuate catabolic gene cluster in Rhodococcus ruber OA1 involved in naphthalene degradation. Ann Microbiol 66(1):469–478
Li P, Ailijiang N, Cao XX, Lei T, Liang P, Zhang XY, Huang X, Teng JL (2015b) Pretreatment of coal gasification wastewater by adsorption using activated carbons and activated coke. Colloids Surf A 482:177–183
Mao W, Li H, Li Y, Zhang H, Qi X, Sun H, Chen Y, Guo S (2009) Chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum (Chlorophyta). Int J Biol Macromol 44(1):70–74
Monnerat G, Seara FA, Evaristo JA, Carneiro G, Evaristo GP, Domont G, Nascimento JH, Mill JG, Nogueira FC, Campos de Carvalho AC (2018) Aging-related compensated hypogonadism: role of metabolomic analysis in physiopathological and therapeutic evaluation. J Steroid Biochem Mol Biol 183:39–50
Moreno MD, García MT, Ventosa A, Iglesias-Guerra F, Mellado E (2010) The extremely halophilic bacterium Halovibrio variabilis IC10 accumulates the compatible solute betaine. Syst Appl Microbiol 33(6):308–310
Moreno MD, Sánchez-Porro C, Piubeli F, Frias L, García MT, Mellado E (2011) Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans. PLoS ONE 6(6):1–8
Neubert H, Bonnert TP, Rumpel K, Hunt BT, Henle ES, James IT (2008) Label-free detection of differential protein expression by LC/MALDI mass spectrometry. J Proteome Res 7(6):2270–2279
Oren A (2002) Molecular ecology of extremely halophilic archaea and bacteria. FEMS Microbiol Ecol 39(1):1–7
Pitcher DG, Saunders NA, Owen RJ (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8(4):151–156
Qu XF, Zheng JT, Zhang YZ (2007) Catalytic ozonation of phenolic wastewater with activated carbon fiber in a fluid bed reactor. J Colloid Interf Sci 309(2):429–434
Rubinstein SM, Kolodkin-Gal I, Mcloon A, Chai L, Kolter R, Losick R, Weitz DA (2012) Osmotic pressure can regulate matrix gene expression in Bacillus subtilis. Mol Microbiol 86(2):426–436
Song J, Sun DD, Wang YN, Xue WP, Dong XL, Ma C, Zhang XX (2010) Acclimatization of halophilic bacteria for biology treatment of high-salt wastewater. Environ Poll Control 32(02):51–54
Vasudevan V, Gayathri KV, Krishnan ME (2018) Bioremediation of a pentacyclic PAH, Dibenz (a, h) Anthracene- A long road to trip with bacteria, fungi, autotrophic eukaryotes and surprises. Chemosphere 202:387–399
Wang W, Han HJ (2012) Recovery strategies for tackling the impact of phenolic compounds in a UASB reactor treating coal gasification wastewater. Bioresour Technol 103(1):95–100
Wang W, Han HJ, Yuan M, Li HQ, Fang F, Wang K (2011a) Treatment of coal gasification wastewater by a two-continuous UASB system with step-feed for COD and phenols removal. Bioresour Technol 102(9):5454–5460
Wang W, Ma WC, Han HJ, Li HQ, Yuan M (2011b) Thermophilic anaerobic digestion of Lurgi coal gasification wastewater in a UASB reactor. Bioresour Technol 102(3):2441–2447
Wang ZX, Xu XC, Gong Z, Yang FL (2012) Removal of COD, phenols and ammonium from Lurgi coal gasification wastewater using A2O-MBR system. J Hazard Mater 235–236:78–84
Wang ZX, Xu XC, Chen J, Yang FJ (2013) Treatment of Lurgi coal gasification wastewater in pre-denitrification anaerobic and aerobic biofilm process. J Environ Chem Eng 1(4):899–905
Wang YF, Wang XL, Li H, Lin KF, Wang P, Yang J, Liu YD, Sun ZY, Fan LH, Wu ZM (2014) Treatment of high salinity phenol-laden wastewater using a sequencing batch reactor containing halophilic bacterial community. Int Biodeterior Biodegrad 93:138–144
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173(2):697–703
Woolard CR, Irvine RL (1994) Biological treatment of hypersaline wastewater by a biofilm of halophilic bacteria. Water Environ Res 66(3):230–235
Yao S, Liu J, Kong XX, Sun H, Liu ZG (2016) Progress in the application of the treatment technology of coal-chemical industrial wastewater. Ind Water Treat 36(3):16–21
Zeidler S, Müller V (2018) The role of compatible solutes in desiccation resistance of Acinetobacter baumannii. MicrobiologyOpen 8(5):e00740
Zhao Q, Han HJ, Xu CY, Zhuang HF, Fang F, Zhang LH (2013) Effect of powdered activated carbon technology on short-cut nitrogen removal for coal gasification wastewater. Bioresour Technol 142:179–185
Zhao DY, Lun WJ, Wei JJ (2017) Discussion on wastewater treatment process of coal chemical industry. IOP Conf Ser Earth Environ Sci 100:012067
Zhu H, Han YX, Xu CY, Han HJ, Ma WW (2018) Overview of the state of the art of processes and technical bottlenecks for coal gasification wastewater treatment. Sci Total Environ 637–638:1108–1126
Zhu LP, Su Y, Ma ZH, Guo LZ, Yang S, Yu H (2022) Comparative proteomic analysis reveals differential protein expression of Hypsizygus marmoreus in response to different light qualities. Int J Biol Macromol 223:1320–1334
Zhuang HF, Han HJ, Hou BL, Jia SY, Zhao Q (2014a) Heterogeneous catalytic ozonation of biologically pretreated Lurgi coal gasification wastewater using sewage sludge based activated carbon supported manganese and ferric oxides as catalysts. Bioresour Technol 166:178–186
Zhuang HF, Han HJ, Jia SY, Hou BL, Zhao Q (2014b) Advanced treatment of biologically pretreated coal gasification wastewater by a novel integration of heterogeneous catalytic ozonation and biological process. Bioresour Technol 166:592–595
Zou YJ, Yang LF, Wang L, Yang SS (2008) Cloning and characterization of a Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus aidingensis AD-6T. J Microbiol 46(4):415–421
Acknowledgements
The research was supported by the Foundation of Tianjin Science and Technology Committee Development Project (No. 21ZYQCSY00050) and Tianjin Key Laboratory Project of Brine Chemical Engineering and Resource Eco-utilization (No. BCERE201908).
Author information
Authors and Affiliations
Contributions
XS: conceptualization, data curation, formal analysis, writing—original draft. GZ: investigation, writing—review and editing. YS: conceptualization, investigation, software. DZ: supervision, resources, data curation. LC: software, visualization.
Corresponding author
Additional information
Communicated by Moracci.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Sun, X., Zhang, G., Shi, Y. et al. Efficient flocculation pretreatment of coal gasification wastewater by halophilic bacterium Halovibrio variabilis TG-5. Extremophiles 28, 11 (2024). https://doi.org/10.1007/s00792-023-01328-w
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00792-023-01328-w