Plasmid diversity in arctic strains of Psychrobacter spp.

Six strains of Psychrobacter spp. isolated from guano of little auks collected on Spitsbergen island (Arctic) carried nine plasmids that were fully sequenced. These replicons (ranging in size from 2917 to 14924 bp) contained either repA (ColE2-type) or repB (iteron-type) replication systems of a relatively narrow host range, limited to Psychrobacter spp. All but one of the plasmids carried predicted mobilization for conjugal transfer systems, encoding relaxases of the MOBQ, MOBV or MOBP families. The plasmids also contained diverse additional genetic load, including a type II restriction-modification system and a gene encoding a putative subunit C of alkyl hydroperoxide reductase (AhpC)—an antioxidant enzyme and major scavenger of reactive oxygen species. Detailed comparative sequence analyses, extended to all plasmids identified so far in psychrophilic bacteria, distinguished groups of the most ubiquitous replicons, which play a key role in horizontal gene transfer in cold environments. Electronic supplementary material The online version of this article (doi:10.1007/s00792-013-0521-0) contains supplementary material, which is available to authorized users.


Introduction
The polar regions occupy over 20 % of the Earth's terrestrial surface. The High Arctic regions, such as the Svalbard Archipelago, are the coldest and most extreme. A greater part (58 %) of the Svalbard Archipelago is permanently covered in snow and ice, and this region also experiences low humidity, strong winds and cyclical, long periods of daylight and darkness (Thomas et al. 2008). Spitsbergen is the largest island of the Svalbard Archipelago in Norway. It lies within the north Arctic tundra and borders the Arctic Ocean, the Norwegian Sea and the Greenland Sea. The Spitsbergen climate is extremely harsh, with a mean annual air temperature of -4.4°C, although due to the frequent winds, usually exceeding 30 m s -1 , the perceived temperature can be even lower. The annual amplitude of temperatures is 49.4°C, with a minimum of -35.9°C, and a maximum of 13.5°C (Nowosielski 2004;Przybylak and Araźny 2006).
Bacteria that tolerate low temperatures are the dominant life forms in polar ecosystems. These cold-adapted microorganisms can be divided into two overlapping ecological groups: (1) psychrophiles, whose cardinal growth temperatures (minimum, optimum and maximum) are 0, 15 and 20°C, respectively, and (2) psychrotrophs (or psychrotolerants), that grow in a broader range of temperatures, between 0 and about 30°C (Morita 1975). Psychrophiles and psychrotolerant microorganisms are subpopulations of bacteria inhabiting various environments including deep sea waters, temporarily or permanently frozen soils, and even food products. Both ecological groups persist in a state of successional dynamics, with one population outcompeting another as environmental conditions change (Helmke and Weyland 2004).
The biodiversity, ecological role and molecular basis of the adaptation to psychrophilicity of microorganisms inhabiting cold environments have been extensively studied in recent years. The increased interest in cold-adapted bacteria is a consequence of biotechnological application of cold-active enzymes (e.g. in the food and chemical industries), which exhibit high catalytic activity at low temperatures and low thermostability at elevated temperatures (Cavicchioli et al. 2002). Over 40 complete genomic sequences of 'psychro' bacteria have been determined so far. These include three strains of the genus Psychrobacter (Psychrobacter arcticus 273-4, Psychrobacter cryohalolentis K5 and Psychrobacter sp. PRwf-1)-bacteria frequently isolated from cold environments, but still largely uncharacterized.
Psychrobacter spp. comprise a group of gram-negative, rod-shaped, non-motile, aerobic and usually non-pigmented, heterotrophic bacteria, which inhabit Arctic and Antarctic ornithogenic soils, polar permafrost, sea ice and water, as well as living organisms (strains have been isolated from krill and fishes) (e.g. Ayala-del-Río et al. 2010;Bozal et al. 2003). Preliminary information concerning the genome composition of these bacteria has been provided by DNA sequencing projects. However, knowledge of their mobile genetic elements (MGE), i.e. plasmids and transposons, is still very fragmentary.
MGEs play a crucial role in horizontal gene transfer (HGT) and may serve as the basis for the construction of genetic tools useful for biotechnology. Until now, only a few such elements have been identified in Psychrobacter spp.: a bacteriophage Psymv2 (Meiring et al. 2012), a composite transposon Tn5080 (7193 bp; contains streptomycin and tetracycline resistance genes) (Petrova et al. 2009) and four plasmids-plasmid 1 (41221 bp) of P. cryohalolentis K5 (composite replicon containing two replication systems), pP62BP1 (34467 bp) of Psychrobacter sp. DAB_AL62B, and pRWF101 (13956 bp) and pRWF102 (2117 bp) of Psychrobacter sp. PRwf-1. Only pP62BP1 (characterized in our previous study) contains a defined genetic module of adaptive value that has been linked with a specific phenotype, namely transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible substrate (organic sulfates metabolism) (Lasek et al. 2012). The other three plasmids seem to be cryptic replicons encoding proteins involved in replication, stabilization and conjugal transfer, as well as hypothetical proteins, mostly of unknown function.
In this study we performed an in-depth characterization of a pool of plasmids of six strains of Psychrobacter spp. isolated from Spitsbergen island in the Arctic, and we show their relationship to other plasmids that have previously been identified in psychrophilic bacteria.

Sample collection and bacterial isolation
Psychrobacter spp. strains were isolated from guano of little auks (dovekie; Alle alle). Guano samples were collected in August 2005 from a breeding colony situated on Spitsbergen island (mountain slope of Ariekammen Isbjornhamna Bay-Hornsund Fjord; 77°00 0 37.0 00 N, 15°31 0 49.5 00 E). For bacterial isolation, 6 independent samples of guano were taken (1 g wet weight each) and homogenized in 10 ml of 0.85 % NaCl (pH 7.0) in conical Pyrex bottles (100 ml) by mixing vigorously with glass beads (120 rpm for 20 min at 5°C). After waiting 20 min to allow larger particles to settle, a series of supernatant dilutions were prepared in saline. Aliquots of 100 ll of the respective dilutions were plated on nutrient agar medium and incubated at 4°C. All operations were carried out aseptically (Zmuda-Baranowska 2010).

16S rRNA gene amplification
A colony PCR method was used for the amplification of 16S rRNA gene fragments (Gathogo et al. 2003). PCR was performed with the primers 27f and 1492r (Lane 1991).
The amplified 16S rDNA fragments were used as templates for DNA sequencing.

DNA sequencing
Nucleotide sequences of Psychrobacter spp. plasmids and PCR-amplified 16S rDNA fragments were determined using a dye terminator sequencing kit and an automated sequencer (ABI 377 Perkin Elmer) in the DNA Sequencing and Oligonucleotide Synthesis Laboratory at the Institute of Biochemistry and Biophysics, Polish Academy of Sciences. Restriction fragments of Psychrobacter plasmids were cloned into compatible sites of vectors pBGS18 and pBluescript SK II for DNA sequencing. Primer walking was used to obtain the full nucleotide sequences of particular plasmids.

DNA manipulations and introduction of plasmid DNA into bacterial cells
Isolation of plasmid DNA and common DNA manipulation techniques were performed as described by Sambrook and Russell (2001). Transformation of E. coli strains was performed according to the method of Kushner (1978). Triparental mating was performed as described previously (Bartosik et al. 2001).

Temperature and pH tolerance
The temperature and pH tolerance of Psychrobacter spp. isolates were measured by following changes in the optical density of cultures (in comparison with noninoculated controls) grown in titration plates, using an automated microplate reader (Sunrise TECAN). Overnight cultures were diluted in fresh LB medium (pH 7.0 for the temperature tolerance analysis and pH 2.0-13.0 for the pH tolerance analysis) to obtain an initial optical density at 600 nm (OD 600 ) of 0.05. Culture aliquots were dispensed into microplates and these were incubated with shaking at 22°C (for pH tolerance analysis) or 4, 15, 22, 25, 30 or 37°C (for temperature tolerance analysis) for 72 h.

Antibiotic, heavy metal and metalloid ion resistance
The minimum inhibitory concentrations (MICs) of ampicillin, chloramphenicol, kanamycin and tetracycline were determined by Epsilometer tests (E tests, bioMérieux), with a gradient of the appropriate antibiotic. Each E test strip was placed on lawns of bacteria on an agar plate. The pattern of bacterial growth was examined after 48 h of incubation at 22°C. The lowest concentration of the antibiotic that prevented growth was considered the MIC.
Analytical grade heavy metal salts (3CdSO 4 9 8H 2 O; CoSO 4 9 7H 2 O; CuSO 4 ; HgCl 2 ; K 2 Cr 2 O 7 ; NaAsO 2 ; Na 2 HAsO 4 9 7H 2 O; NiCl 2 9 6H 2 O; ZnSO 4 9 7H 2 O) were used to prepare 0.01, 0.1 or 1 M stock solutions in water. Each solution was filter-sterilized and added to LB medium to final concentrations of between 0.01 and 100 mM of the metal ion. Exact MICs for the Psychrobacter isolates were defined on titration plates using a broth dilution method (Sunrise, TECAN) for measuring changes in the optical density of the cultures in comparison with non-inoculated controls. Each microplate was checked for growth at 24-h intervals for 3 days.

Results
Isolation and identification of a pool of strains of Psychrobacter spp.
Bacterial strains were isolated from guano of little auksthe most abundant Arctic sea birds. A random selection of 88 isolates (Table S1, Supplementary Materials) (obtained from 6 independent guano samples) was examined for their colony and cell morphology, as well as basic physiological features (data not shown). Fragments of 16S rDNA were amplified by PCR from the strains and sequenced. Comparative analysis of the obtained sequences revealed that 17 of the strains (19.3 %) could be classified to the genus Psychrobacter. One of the randomly chosen strains (Psychrobacter sp. DAB_AL62B) was already described in our previous study (Lasek et al. 2012). The remaining strains were subjected to further analysis. To avoid the characterization of strains of clonal origin, isolates from different guano samples were examined. Phylogenetic analysis was performed, based on the comparison of partial 16S rDNA sequences (1351 bp) of the strains and type strains representing the 34 Psychrobacter species described to date. The analyzed sequences of the strains DAB_AL32B, DAB_AL43B, DAB_AL60 and DAB_AL109bw were identical, and share 99.70 % similarity with DAB_AL25. The topology of the phylogenetic tree revealed that these five strains form a separate cluster linked to P. frigidicola DSM 12411 (99.11 % identity of the 16S rDNA sequences), isolated from ornithogenic soil in Antarctica (Bowman et al. 1996) (Fig. 1). The strains DAB_AL62B and DAB_AL12 were clustered in different groups. The former is most related to P. urativorans DSM 14009 (99.26 % identity) and P. cibarius JG-219 (99.18 % identity), isolated from ornithogenic soil and food products, respectively (Bowman et al. 1996;Jung et al. 2005), while the latter (most divergent among the ''DAB'' strains) is closest to P. cryohalolentis K5 (99.78 % identity), isolated from a cryopeg taken from the permafrost in the Kolyma lowland (Siberia, Russia) (Bakermans et al. 2006) (Fig. 1).

Characterization of the Psychrobacter spp. strains
A preliminary characterization of the isolated Psychrobacter spp. strains revealed that all of them fulfill the requirements of psychrophilicity (Morita 1975): they could grow at temperatures ranging between 4 and 25°C, but not at C30°C (optimum temperature 22°C) ( Figure S1, Supplementary Materials). These strains also grew in LB medium at pH values between 5 and 10, which is typical for neutrophilic bacteria (Slonczewski et al. 2009).
Possible resistance phenotypes, which are often determined by plasmids and transposons, were also examined.
None of the Psychrobacter spp. strains were resistant to any of the tested antibiotics (ampicillin, chloramphenicol, kanamycin and tetracycline), but they showed resistance to several heavy metals: (1) low or moderate resistance to zinc, chromium(VI) and copper (MICs from 2 to 4 mM), and (2) moderate or high level resistance to arsenate-As(V) (MICs from 15 to 100 mM), except DAB_AL25 (MIC 2 mM). Two strains (DAB_AL12 and DAB_AL32B) also exhibited trigger level resistance to arsenite-As(III) (MICs of 1.5 and 2 mM, respectively).
Identification of a pool of Psychrobacter spp. plasmids Plasmids are natural vectors that play a major role in the dissemination of accessory genetic information in HGT. The Psychrobacter strains analyzed in this study were found to carry nine circular plasmids (ranging in size from 2.9 to 14.9 kb) that are listed in Table 1. The highest number of plasmids was found in DAB_AL43B (4) and DAB_AL60 (2), while the remaining strains possess only single replicons or none at all.
The complete nucleotide sequences of these plasmids were determined. The relatively low average GC content of the obtained sequences (35.7-42.9 mol%) is typical for Psychrobacter spp. genomic DNA (e.g. Ayala-del-Río et al. 2010). The plasmids were found to contain from 2 to 13 open reading frames (ORFs), of sizes between 165 and 2133 bp (Table 1). Based on similarities to known genes, it was possible to predict functions for the polypeptides encoded by almost half of these ORFs. A summary of the ORFs, including their position, transcriptional orientation, the size of the encoded proteins, and their closest known homologs, is presented in Table S2 (Supplementary Materials).
Further analysis of the organization of the plasmids revealed the presence of several putative genetic modules responsible for (1) plasmid replication (REP), (2) stabilization (STA), and (3) mobilization for conjugal transfer (MOB). All plasmids also appear to carry different accessory genetic information (Fig. 2).
The characterized REP modules of pP12P1, pP43BP1, pP43BP2, pP43BP3 and pP60P2 have a structure that is typical for the replication systems of many theta-replicating plasmids (Chattoraj 2000). They contain a single ORF encoding a predicted protein with similarities to the initiator RepB protein, possessing nicking-closing (topoisomerase I like) activity, and a putative origin of replication (oriV), placed upstream of the repB gene (Fig. 3a). Comparative analysis revealed that closely related replication proteins are also encoded by plasmid 1 of P. cryohalolentis K5 (acc. no. NC_007968) and many plasmids of Acinetobacter spp. strains (e.g. pMAC and p11921 of Acinetobacter baumannii, acc. nos. NC_006877 and GU979000, respectively).
The putative oriVs of pP12P1, pP43BP1, pP43BP2, p43BP3 and pP60P2 contain four tandemly placed 22-bp long repeated sequences (IT1-IT4), i.e. putative iterons, which (as shown for other related plasmids of this type; Chattoraj 2000) may constitute binding sites for the Rep proteins. The IT repeats are located 81-132 bp upstream of the repB genes. They are identical in particular plasmids (with one exception being IT4 of pP43BP2, which differs from IT1 to IT3 by 5 mismatches); however, they show only limited reciprocal sequence similarity (Fig. 3a). Interestingly, the plasmids pP12P1, pP43BP1, pP43BP2 and pP60P2 contain a conserved palindromic sequence [5 0 -TAA(A/C)AGCTTTTA-3 0 ] located 37-46 bp upstream of the IT1 repeats (Fig. 3a). In the case of pP43BP3, a similar sequence (5 0 -AAATTCATTT-3 0 ) is situated between the IT2 and IT3 repeats (Fig. 3a). High conservation of the palindromic structure might suggest a role in replication initiation.
The REP modules of the second group of plasmids (pP32BP1, pP43BP4, pP60P1 and pP109bwP1) contain a single ORF encoding a putative protein in which three conserved regions can be distinguished: (1) the replicase domain, typical for plasmid DNA replication initiator proteins, (2) an alpha helical domain, found in the C-terminal regions of primases (PriCT-1), and (3) a HTH motif, that is most probably responsible for protein-DNA interactions (data not shown). The analyzed proteins share significant amino acid sequence homology with the RepA proteins of plasmid 1 of P. cryohalolentis K5 and  (Yasueda et al. 1989). Careful inspection of the nucleotide sequences of the analyzed REP modules also revealed the presence of three DNA regions typical for the origins of replication of ColE2-type plasmids: (1) two direct repeats (L and R) 5 0 -CAGATAA-3 0 , (2) sites a and b, which determine the specificity of the interactions of Rep protein with the origin, and (3) a short sequence to which the Rep protein synthesizes a unique RNA primer that is crucial for the initiation of leading-strand DNA synthesis by DNA polymerase I (Fig. 3b) (Yagura et al. 2006). The host range of REP modules representing both of the aforementioned groups of replicons was then examined. For this analysis we used two shuttle plasmids, pABW-12P1 and pABW-60P1, containing the REPs of pP60P1 and pP12P1, respectively, cloned (within XbaI restriction fragments) into the E. coli-specific, narrow host range (NHR) vector pABW1 (ColE1-type ori of pMB1). The ability of the shuttle plasmids to replicate was tested in (1) A. tumefaciens LBA 288R and P. versutus UW225 (Alphaproteobacteria), (2) Alcaligenes sp. LM16R (Betaproteobacteria), and (3) P. putida KT2442R (Gammaproteobacteria). The plasmids pABW-12P1 and pABW-60P1 were found to replicate exclusively in Psychrobacter spp., suggesting a relatively NHR.

Stable maintenance modules
Most of the analyzed plasmids appeared to lack stabilization systems, which are components of the vast majority of bacterial replicons. Only plasmid pP43BP3 carries a complete toxin-antitoxin (TA) system, possibly involved in the postsegregational elimination of plasmid-less cells from a bacterial population (Fig. 2). This putative TA module is composed of two short overlapping ORFs (14 bp overlap) encoding proteins with similarity to a number of RelB-like antitoxins (ORF5) and RelE-like toxins (ORF4) of relBE/ parDE-type TA systems (Anantharaman and Aravind 2003). Two other plasmids, pP43BP2 and pP109bwP1, carry incomplete TA modules represented by single ORFs encoding RelB-like antitoxins.
Another plasmid, pP60P2, contains a putative type II restriction-modification (R-M) system. Similar to TA, such systems may increase the stability of plasmids by killing plasmid-less cells (e.g. Ichige and Kobayashi 2005;Dziewit et al. 2011b). The RM module of pP60P2 is composed of two divergently orientated ORFs: ORF5 and ORF6 (Fig. 2). BLAST searches revealed that the polypeptide encoded by ORF5 shares substantial homology with a large number of proteins annotated as m5C methyltransferases (MTases), with highest sequence similarity (60 %) to a putative MTase of Marivirga tractuosa DSM 4126 (acc. no. YP_004054233). The ORF6-encoded protein is similar to a Fig. 2 Linear maps showing the genetic structure of circular plasmids pP12P1, pP32BP1, pP43BP1, pP43BP2, pP43BP3, pP43BP4, pP60P1, pP60P2 and pP109bwP1. The predicted genetic modules are indicated by gray rectangles: REP A (REP B ) repA-like (repB-like) replication systems, MOB P (MOB Q , MOB V ) system for mobilization for conjugal transfer, TA toxin-antitoxin system, R-M type II restriction-modification system, MRS multimer resolution system, AhpC subunit C of alkyl hydroperoxide reductase module. Gray stars indicate Sel1 domain repeat-containing proteins with slight homology to beta-lactam hydrolases. Arrows indicate the transcriptional orientation of the genes. Areas of gray shading connect genes of different plasmids encoding related proteins putative restriction endonuclease (predicted recognition sequence 5 0 -CGCG-3 0 ) encoded by Moraxella catarrhalis BC1 (acc. no. ZP_11632318).
ORF10 of pP60P2 (Fig. 2) encodes a predicted protein with a catalytic domain characteristic of serine recombinases, often recognized as resolvases in multimer resolution systems (MRS). MRS act to resolve plasmid oligomers and this activity increases the number of independent plasmid molecules available for distribution during cell division (Bahl et al. 2009). Homologs of ORF10 have been identified in other Psychrobacter plasmids, including pRWF101 of PRwf-1 and pP62BP1 of DAB_AL62B (Lasek et al. 2012).

MOB modules: structure and diversity
Many bacterial plasmids can be mobilized for conjugation by other self-transmissible elements (e.g. conjugative plasmids and integrative and conjugative elements-ICEs) encoding type 4 secretion systems. The mobilizable plasmids contain MOB modules encoding specific relaxosome components and an origin of transfer (oriT) (Francia et al. 2004;Garcillan-Barcia et al. 2009). The analyzed plasmids of Psychrobacter spp. were found to contain eight putative MOB modules, which may be classified, based on amino acid sequence similarities of their relaxases, into three distinct families (MOB P , MOB Q and MOB V ).
Analysis of the MOB P family [MOB P5 (MOB HEN ) clade] members, found in plasmids pP43BP1, pP43BP4, pP60P1 and pP109bwP1, revealed their structural divergence and permitted the identification of two subgroups. The single member of the first subgroup (MOB of pP43BP1) is composed of two overlapping ORFs (putative mobA and mobC), while the other three MOB P modules carry four convergently orientated ORFs (Fig. 4). The MobA relaxases of the two subgroups show partial amino acid sequence identity (B36 %). The pP43BP1 MobA is most similar (44 % identity) to the relaxase/mobilization nuclease domain protein of plasmid pRWF101 of Psychrobacter sp. PRwf-1 (acc. no. NC_009516). In turn, the MobA proteins of pP43BP4, pP60P1 and pP109bwP1 are most similar (C57 % identity) to the relaxase encoded by plasmid pKW1 of Pseudoalteromonas sp. 643A (Cieśliński et al. 2008). Interestingly, the three relaxases of this second subgroup are at least twice the length of other MobA proteins, and their C-terminal parts show no similarity to known protein sequences present in the GenBank (NCBI) databases. All of the analyzed MOB HEN modules contain predicted oriTs (located upstream of the mobC genes), which share sequence similarity with oriT of the mobilization system (MOB HEN clade member) of plasmid pSW200 of Erwinia stewartii SW2 (Fu et al. 1998) (Fig. 4).  The MOB Q family is the most abundant and diverse group of mobilization systems. Two such modules (encoding relaxases most similar to proteins classified within the MOB Q3 clade; Garcillan- Barcia et al. 2009) were identified within plasmids pP32BP1 and pP60P2. Both MOBs are composed of two non-overlapping, divergently orientated ORFs, encoding a putative relaxase (MobA) and a mobilization protein C (MobC), as well as a predicted oriT located within the mobA-mobC intergenic region (Fig. 4). The oriTs display 55 % sequence identity and they match the oriT consensus sequence of the MOB Q family (Francia et al. 2004) (Fig. 4). The relaxases of pP32BP1 and pP60P2 differ significantly in length and they exhibit only a moderate level of sequence similarity (40 %). Similarity searches revealed that the predicted proteins are most closely related (52 %) to the MobA protein encoded by a small (4658 bp) cryptic plasmid pMbo4.6 of Moraxella bovis ATCC 10900 (acc. no. NC_013500).
The MOB modules of plasmids pP43BP2 and pP43BP3 (Fig. 4) encode related relaxases (85 % identity), which exhibit C40 % amino acid sequence homology with the Mob protein of the mobilization system of a broad host range plasmid pBBR1 (2687 bp) of Bordetella bronchiseptica S87-the prototype of the MOB V2 clade (MOB V family) (Antoine and Locht 1992;Garcillan-Barcia et al. 2009). The predicted oriTs of these plasmids (both placed upstream of the mobA genes) are identical, and differ slightly from the oriT of pBBR1 (Fig. 4). They contain 8-bp long inverted repeats (IR) separated by a 3-bp spacer, which is a typical structure for oriTs of MOBs of the MOB V family (Szpirer et al. 2001;Guzman and Espinosa 1997).

Accessory genetic information
Besides the REP, MOB and STA modules, the analyzed plasmids were found to contain accessory genetic information (33 ORFs in total) of unknown function. Using the PRIAM program for automated enzyme detection we were able to assign putative functions to only four of the identified ORFs. ORF4 and ORF8 of pP60P2 and ORF6 of pP32BP1 (Fig. 2) encode putative Sel1 domain proteins, with weak homology (35-39 % amino acid sequence similarity; E value [1e-10) to beta-lactam hydrolases (EC 3.5.2.6)-a group of enzymes of varying specificity that hydrolyze penicillins or cephalosporins. ORF7 of plasmid pP60P2 encodes a putative protein with 99 % amino acid sequence similarity to subunit C of alkyl hydroperoxide reductase (EC 1.11.1.15) encoded by the ahpC gene of Psychrobacter arcticus 273-4. This enzyme reduces H 2 O 2 , organic peroxides and peroxynitrite (ONOO -), and therefore acts as an antioxidant and major scavenger of reactive oxygen species (ROS) generated in the cytoplasm of bacteria as a by-product of aerobic metabolism (Chen et al. 1998;Steele et al. 2010).

Discussion
In this study we have performed a detailed analysis of the plasmidome of six Arctic strains of Psychrobacter spp. The effect of temperature and pH on the growth of these strains in culture was similar, which permitted their classification as neutrophilic psychrophiles. The strains also showed a low or moderate level of resistance against arsenic(III), zinc, chromium(VI) and copper, and a high level of tolerance to arsenic(V). Since planktivorous seabirds are efficient vectors for the transport of heavy metals between marine and terrestrial ecosystems (e.g. Yin et al. 2008), these resistance phenotypes may be a consequence of the constant exposure of bacteria to heavy metals accumulated in little auk feces. Nevertheless, none of the plasmids analyzed in this study contain heavy metal resistance determinants.
The isolated Psychrobacter spp. strains carried nine plasmids in total, containing either a repA-like (pP32BP1, pP43BP4, pP60P1 and pP109bwP1) or repB-like (pP12P1, pP43BP1, pP43BP2, pP43BP3 and pP60P2) replication system. REP modules are major components of plasmid backbones, and therefore they serve as exclusive phylogenetic markers for classification of these replicons (e.g. Petersen et al. 2009). We performed in-depth searches of the NCBI databases in order to define all known plasmids of psychrophilic bacteria. This analysis revealed the presence of only 45 plasmids (including the 9 replicons analyzed in this study) identified in 27 bacterial strains (the vast majority of the plasmid sequences were derived from whole genome sequencing projects).
All plasmids of Psychrobacter spp. analyzed in this study have a relatively NHR, limited to this genus. Eight contain MOB systems (classified within the MOB Q , MOB V or MOB P families), which suggests the possibility of their mobilization by conjugal transfer. As shown by Smorawinska et al. (2012) such NHR, mobilizable plasmids are efficient carrier molecules, since they may act as natural suicide vectors, promoting the spread of diverse genetic information among evolutionarily distinct bacterial species.
Each of the analyzed plasmids also carries an additional genetic load. Most of the predicted ORFs encode Phylogenetic tree for 16S rDNA sequences of psychrophilic bacteria harboring plasmids. The tree was constructed by the neighbor-joining algorithm with Kimura corrected distances, and the statistical support for the internal nodes was determined by 1000 bootstrap replicates. The 16S rDNA sequence of a representative of the Archaea, Ignicoccus hospitalis KIN4/I, was used as the outgroup. Gram-negative and gram-positive bacteria are shown against dark and light gray backgrounds, respectively. The type of replication system of each analyzed plasmid (based on sequence alignments, which are summarized in the Supplementary Materials, Figs. S2, S3 and S4) is indicated by a dot or square: black dot RepB-like, white square RepA-like, black square RepL-like, white dot other types. Dots within square parenthesis represent replication systems of composite replicons, and dots within round parenthesis represent replication modules of plasmids of Pseudomonas sp. MC1, Pseudoalteromonas spp. (Bsi429, PS1M2 and PS1M3) and Flavobacterium sp. KP1 (whose 16S rDNA sequences were not available in the GenBank database). Accession numbers of all plasmids whose replication proteins were used for the analysis are given in the Supplementary Materials, Table S3 hypothetical proteins of unknown function (this is also the case for Psychrobacter spp. genomes, which are largely uncharacterized and require further investigation). Two of the analyzed plasmids (pP32BP1 and pP60P2) carry ORFs encoding proteins with Sel1 domains (present in beta-lactam hydrolases), but the host strains of these plasmids did not show resistance to beta-lactam antibiotics. Nevertheless, this is an interesting observation, since the beta-lactamase-like proteins, possibly involved in some aspect of ''normal'' bacterial metabolism, may in fact represent ancestors of the antibiotic resistance determinants commonly found in environmental strains and clinical isolates (Petrova et al. 2009).
One of the analyzed plasmids (pP60P2) encodes a putative type II restriction-modification system. The main role of R-M systems is to protect the host cell against invasion by exogenous (not protected by methylation) DNA, e.g. bacteriophage genomes (Tock and Dryden 2005). The first bacteriophage able to infect Psychrobacter spp. cells was recently described (Meiring et al. 2012). On the other hand, R-M systems may facilitate DNA recombination by the generation of highly recombinogenic, double-stranded breaks in homologous donor DNA, which can significantly enhance bacterial diversity. R-M systems are frequently carried by plasmids, e.g. pP62BP2 of Psychrobacter DAB_AL62B contains two nearly identical modules of this type (Lasek et al. 2012). It has been demonstrated that R-M systems act in an analogous manner to TA modules, which stabilize plasmids by eliminating plasmid-less cells from the bacterial population (Kobayashi 2001).
In close proximity to the R-M module, plasmid pP60P2 also contains a single ORF encoding a putative subunit C of alkyl hydroperoxide reductase (AhpC). The AhpC proteins are components of alkyl hydroperoxide reductase complexes-AhpCD or AhpCF. Peroxiredoxins of the AhpC family reduce, and therefore, detoxify H 2 O 2 , organic peroxides and peroxynitrite (ONOO -), while AhpD and AhpF are peroxiredoxin reductases that restore the enzymatic activity of AhpC. AhpCD and AhpCF complexes serve as important antioxidants, and the AhpC protein is the major scavenger of ROS produced by aerobic metabolism (Chen et al. 1998;Steele et al. 2010). In a few cases it has been demonstrated that AhpC proteins may influence the virulence of bacterial pathogens, including Helicobacter pylori (Olczak et al. 2003) and Mycobacterium bovis (Wilson et al. 1998). Interestingly, it was also shown that mycobacterial AphC could protect human cells against necrosis and apoptosis caused by reactive nitrogen intermediates (RNI) (Chen et al. 1998).
The role of the AhpC proteins in psychrophilic strains may be strictly linked to the environmental conditions they face. Springtime polar ozone depletion and global warming mean that the impact of UV radiation (UVR) remains relatively high in polar regions. This phenomenon, together with other stress-inducing factors (e.g. pollutants), strongly enhances the formation of ROS (Müller et al. 2012;Regoli et al. 2000). Therefore, the acquisition of an ahpC-encoding plasmid may potentially increase the adaptation of the host strain to the arctic environment. Further study is required to experimentally confirm this speculation.
In conclusion, the findings of this study greatly increase current knowledge of the mobile DNA of Psychrobacter spp. This extended comparative analysis of plasmids has shed light on the distribution of related replicons among psychrophilic bacteria, which, in many cases, reflects the frequency and direction of HGT events. Our results also identify the most ubiquitous ''cold-active'' plasmid-encoded REP modules, which may form the basis of novel shuttle vectors, specific for this group of bacteria.