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Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures

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Abstract

We investigated the pressure tolerance of deep-sea eel (Simenchelys parasiticus; habitat depth, 366–2,630 m) cells, conger eel (Conger myriaster) cells, and mouse 3T3-L1 cells. Although there were no living mouse 3T3-L1 and conger eel cells after 130 MPa (0.1 MPa = 1 bar) hydrostatic pressurization for 20 min, all deep-sea eel cells remained alive after being subjected to pressures up to 150 MPa for 20 min. Pressurization at 40 MPa for 20 min induced disruption of actin and tubulin filaments with profound cell-shape changes in the mouse and conger eel cells. In the deep-sea eel cells, microtubules and some actin filaments were disrupted after being subjected to hydrostatic pressure of 100 MPa and greater for 20 min. Conger eel cells were sensitive to pressure and did not grow at 10 MPa. Mouse 3T3-L1 cells grew faster under pressure of 5 MPa than at atmospheric pressure and stopped growing at 18 MPa. Deep-sea eel cells were capable of growth in pressures up to 25 MPa and stopped growing at 30 MPa. Deep-sea eel cells required 4 h at 20 MPa to finish the M phase, which was approximately fourfold the time required under atmospheric conditions.

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Acknowledgements

This study was supported by Grant-in-Aid for Young Scientist (A) (No. 14704007) and Grant-in-Aid for Exploratory Research (No. 15656214) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan

    Sumihiro Koyama & Tetsuya Miwa

  2. Department of Biological Information, Graduate School of Bioscience and Biotechnology,Tokyo Institute of Technology, Nagatsuta, Yokohama 226-8501, Japan

    Hiromi Kobayashi

  3. Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma 374-0193, Japan

    Akira Inoue

  4. Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8550, Japan

    Masuo Aizawa

Authors
  1. Sumihiro Koyama
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  2. Hiromi Kobayashi
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  3. Akira Inoue
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  4. Tetsuya Miwa
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  5. Masuo Aizawa
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Correspondence to Sumihiro Koyama.

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Communicated by K. Horikoshi

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Koyama, S., Kobayashi, H., Inoue, A. et al. Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures. Extremophiles 9, 449–460 (2005). https://doi.org/10.1007/s00792-005-0462-3

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  • DOI: https://doi.org/10.1007/s00792-005-0462-3

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