Skip to main content

Advertisement

SpringerLink
Log in

Effect of antioxidant lycopene on human osteoblasts

  • Research
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

The aim of this in vitro study is to evaluate the effect of antioxidant lycopene on human osteoblasts.

Material and method

The human osteoblast cell line (CRL-11372) was obtained from the American Type Culture Collection (ATCC Manassas, Va) and grown in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 U/ml), and streptomycin (100 mg/ ml) at 37 °C in a humidified atmosphere of 5% CO2 and 95% air. The effective dose of lycopene was determined by MTT assay and a real-time cell analysis (RTCA) system. Proliferative effects were analyzed by in vitro wound healing model. Gene expressions of type 1 collagen (COL1A1), osteocalcin (OCN), and growth differentiation factor-5 (GDF-5) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) at 72 h. Statistical differences between test groups were analyzed with a one-way ANOVA test.

Results

MTT assay showed that the doses between 10−5 and 1 µmol of lycopene had dose-dependent proliferative effects. The doses between 10−5 and 10−1 µmol were most effective at 72 h. Lycopene accelerates the healing rate by increasing osteoblast proliferation.

Conclusion

Results suggested that lycopene had proliferative effects on human osteoblasts, which may help to increase bone regeneration, and thus, it can be useful in tissue engineering procedures.

Clinical relevance

By the help of antioxidants like lycopene capacity, velocity and quality of new bone forming may be increased in periodontal and dental implant treatments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

Not applicable.

Notes

  1. CRL-11372 cell line, ATCC Manassas, Va.

  2. Lycopene, Sigma, Israel.

  3. THF, Sigma, Israel.

  4. Biological Industries, Beith Haemek, Israel.

  5. Roche Applied Science, Mannheim, Germany.

  6. Ibidi USA Inc., in Fitchburg, Wisconsin

  7. High Pure RNA Isolation Kit, Roche, Mannheim, Germany

  8. Roche, Mannheim, Germany

  9. SPSS Inc., Chicago, IL

References

  1. Van Dyke TE (2008) The management of inflammation in periodontal disease. J Periodontol 79:1601–1608

    Article  PubMed  PubMed Central  Google Scholar 

  2. Hathaway-Schrader JD, Novince CM (2000) (2021) Maintaining homeostatic control of periodontal bone tissue. Periodontol 86:157–187

    Article  Google Scholar 

  3. Kapila YL (2000) (2021) Oral health’s inextricable connection to systemic health: Special populations bring to bear multimodal relationships and factors connecting periodontal disease to systemic diseases and conditions. Periodontol 87:11–16

    Article  Google Scholar 

  4. Pamuk F, Kantarci A (2022) Inflammation as a link between periodontal disease and obesity. Periodontology 2000 90(1):186–196

  5. Yucel-Lindberg T, Båge T (2013) Inflammatory mediators in the pathogenesis of periodontitis. Expert Reviews in Molecular Medicine 15(e-7):1–22

  6. Sczepanik FSC, Grossi ML, Casati M, Goldberg M, Glogauer M (2000) Fine N and Tenenbaum HC (2020) Periodontitis is an inflammatory disease of oxidative stress: we should treat it that way. Periodontol 84:45–68

    Article  Google Scholar 

  7. Dahiya P, Kamal R, Gupta R, Bhardwaj R, Chaudhary K, Kaur S (2013) Reactive oxygen species in periodontitis. J Indian Soc Periodontol 17:411

    Article  PubMed  PubMed Central  Google Scholar 

  8. Sharma A, Sharma S (2011) Reactive oxygen species and antioxidants in periodontics: a review. Int J Dent Clin 3:44–48

    Google Scholar 

  9. Andrei M, Dinischiotu A, Didilescu AC, Ionita D, Demetrescu I (2018) Periodontal materials and cell biology for guided tissue and bone regeneration. Annals of Anatomy-Anatomischer Anzeiger 216:164–169

    Article  Google Scholar 

  10. Sulijaya B, Takahashi N, Yamazaki K (2019) Host modulation therapy using anti-inflammatory and antioxidant agents in periodontitis: A review to a clinical translation. Arch Oral Biol 105:72–80

    Article  PubMed  Google Scholar 

  11. Castro MML, Duarte NN, Nascimento PC, Magno MB, Fagundes NCF, Flores-Mir C, Monteiro MC, Rösing CK, Maia LC, Lima RR (2019) Antioxidants as adjuvants in periodontitis treatment: a systematic review and meta-analysis. Oxidative Med Cell Longev 9187978

  12. Corrêa M, Pires P, Ribeiro F, Pimentel S, Casarin R, Cirano F, Tenenbaum H, Casati M (2017) Systemic treatment with resveratrol and/or curcumin reduces the progression of experimental periodontitis in rats. J Periodontal Res 52:201–209

    Article  PubMed  Google Scholar 

  13. Rao AV, Ray M, Rao L (2006) Lycopene. Adv Food Nutr Res 51:99–164

    Article  PubMed  Google Scholar 

  14. Teodoro AJ, Oliveira FL, Martins NB, Maia GdA, Martucci RB, Borojevic R (2012) Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines. Cancer Cell Int 12:1–9

    Article  Google Scholar 

  15. Chen J, Song Y, Zhang L (2013) Effect of lycopene supplementation on oxidative stress: an exploratory systematic review and meta-analysis of randomized controlled trials. J Med Food 16:361–374

    Article  PubMed  PubMed Central  Google Scholar 

  16. Kim L, Rao AV, Rao LG (2003) Lycopene II—effect on osteoblasts: the carotenoid lycopene stimulates cell proliferation and alkaline phosphatase activity of SaOS-2 cells. J Med Food 6:79–86

    Article  PubMed  Google Scholar 

  17. Russo C, Ferro Y, Maurotti S, Salvati MA, Mazza E, Pujia R, Terracciano R, Maggisano G, Mare R, Giannini S (2020) Lycopene and bone: an in vitro investigation and a pilot prospective clinical study. J Transl Med 18:1–11

    Article  Google Scholar 

  18. Tripathi P, Blaggana V, Upadhyay P, Jindal M, Gupta S, Nishat S (2019) Antioxidant therapy (lycopene and green tea extract) in periodontal disease: a promising paradigm. J Indian Soc of Periodontol 23:25

    Article  Google Scholar 

  19. Viswa Chandra R, Sandhya YP, Nagarajan S, Harish Reddy B, Naveen A, Murthy KRV (2012) Efficacy of lycopene as a locally delivered gel in the treatment of chronic periodontitis: smokers vs nonsmokers. Quintessence Int 43(5):401–411

  20. Chandra RV, Srinivas G, Reddy AA, Reddy BH, Reddy C, Nagarajan S, Naveen A (2013) Locally delivered antioxidant gel as an adjunct to nonsurgical therapy improves measures of oxidative stress and periodontal disease. J Periodont Implant Sci 43:121–129

    Article  Google Scholar 

  21. Rao SM, Ugale GM, Warad SB (2013) Bone morphogenetic proteins: periodontal regeneration. N Am J Med Sci 5:161

    Article  PubMed  PubMed Central  Google Scholar 

  22. Moore YR, Dickinson DP, Wikesjö UM (2010) Growth/differentiation factor-5: a candidate therapeutic agent for periodontal regeneration? A review of pre-clinical data. J Clin Periodontol 37:288–298

    Article  PubMed  Google Scholar 

  23. Jin L, Li X (2013) Growth differentiation factor 5 regulation in bone regeneration. Curr Pharm Des 19:3364–3373

    Article  PubMed  Google Scholar 

  24. Florencio-Silva R, Sasso GRdS, Sasso-Cerri E, Simões MJ, Cerri PS (2015) Biology of bone tissue: structure, function, and factors that influence bone cells. BioMed Res Int 2015:421746

  25. Nizam N, Discioglu F, Saygun I, Bal V, Avcu F, Ozkan CK, Serdar MA (2014) The effect of α-tocopherol and selenium on human gingival fibroblasts and periodontal ligament fibroblasts in vitro. J Periodontol 85:636–644

    Article  PubMed  Google Scholar 

  26. Öztürk F, Malkoc S, Ersöz M, Hakki SS, Bozkurt BS (2011) Real-time cell analysis of the cytotoxicity of the components of orthodontic acrylic materials on gingival fibroblasts. Am J Orthod Dentofac Orthop 140:e243–e249

    Article  Google Scholar 

  27. Zhang H, Chen X, Wang D, Zheng X, Chen Z (2007) The effect of lycopene on osteoblasts which have incurred oxidative stress. Chinese J Osteoporosis 13:86

    Google Scholar 

  28. Hughes FJ, Turner W (2000) Belibasakis G and Martuscelli G (2006) Effects of growth factors and cytokines on osteoblast differentiation. Periodontol 41:48–72

    Article  Google Scholar 

  29. Griffiths G, Moulson A, Petrie A, James I (1998) Evaluation of osteocalcin and pyridinium crosslinks of bone collagen as markers of bone turnover in gingival crevicular fluid during different stages of orthodontic treatment. J Clin Periodontol 25:492–498

    Article  PubMed  Google Scholar 

  30. Lee J, Wikesjö UM (2014) Growth/differentiation factor-5: pre-clinical and clinical evaluations of periodontal regeneration and alveolar augmentation–review. J Clin Periodontol 41:797–805

    Article  PubMed  Google Scholar 

Download references

Funding

This study is supported by the Gulhane Military Medical Academy, Ankara, Turkey, (Project no: AR2013/31).

Author information

Authors and Affiliations

  1. Gulhane Faculty of Dental Medicine, University of Health Sciences, Ankara, Turkey

    U Bengi V., I Saygun & E Ozcan

  2. Ministry of Health of Turkey, 75Th Year Oral Dental Health Hospital, Ankara, Turkey

    V Bal

  3. Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey

    C Kose Ozkan

  4. Gulhane Faculty of Medicine, University of Health Sciences, Ankara, Turkey

    D Torun

  5. Memorial Ankara Hospital, Ankara, Turkey

    F Avcu

  6. The Forsyth Institute, Cambridge, MA, USA

    A Kantarcı

Authors
  1. U Bengi V.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I Saygun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V Bal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E Ozcan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C Kose Ozkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D Torun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F Avcu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A Kantarcı
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Vahdi Umut Bengi and Isil Saygun conceived the presented idea and developed the theory.

Vahdi Umut Bengi wrote the manuscript with support from Isil Saygun, Vehbi Bal, and Ferit Avcu.

Cansel Kose Ozkan and Deniz Torun performed the analysis and designed the tables and figures.

Erkan Ozcan and Alpdogan Kantarcı contributed to the design and implementation of the research, to the analysis of the results, and to the writing of the manuscript.

All authors reviewed the manuscript.

Corresponding author

Correspondence to U Bengi V..

Ethics declarations

Ethical approval

This study has been ethically approved by the Gulhane Military Medical Academy Ethics Committee on the 3rd of April 2013.

Competing interests

The authors report no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bengi V., U., Saygun, I., Bal, V. et al. Effect of antioxidant lycopene on human osteoblasts. Clin Oral Invest 27, 1637–1643 (2023). https://doi.org/10.1007/s00784-022-04789-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-022-04789-z

Keywords

Search

Navigation