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Effect of relative centrifugal force on the biological properties of liquid platelet-rich fibrin produced via horizontal centrifugation

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Abstract

Objectives

Platelet-rich fibrin (PRF) in liquid form has shown advantages in tissue engineering including acting as injectable fillers and drug carriers. However, few studies have investigated the best relative centrifugal force (RCF) for preparing liquid PRF. The aim of the present study was to find out optimal centrifugation force for preparing liquid PRF.

Materials and methods

Liquid PRF was prepared using horizontal centrifugation (liquid H-PRF) with RCF ranging from 100 g, 300 g, 500 g, to 700 g for 8 min. The volume, weight, solidification time, and tensile properties were subsequently investigated. Scanning electron microscopy (SEM) and rheologic tests were carried out to investigate the microstructure and rheologic properties of liquid H-PRF after natural polymerization. The total number, concentration, and distribution of cells within each liquid H-PRF was evaluated by complete blood count (CBC) analysis and hematoxylin–eosin staining.

Results

As RCF values increased, the volume and weight of liquid H-PRF both increased accordingly. SEM images revealed that as the centrifugal force increased, the fibrin bundles became thinner with a denser fibrin network, and rheologic tests revealed improved mechanical properties. CBC analysis demonstrated that 500 g group had the highest number of leukocytes and neutrophils, whereas 100 g group yielded the highest concentration of leukocytes and platelets. Furthermore, histological analysis suggests that cells obtained by 500 g for 8 min were most evenly distributed in liquid H-PRF.

Conclusions

In summary, the present study provided insights into the contents of liquid H-PRF prepared at different centrifugation forces, enabling clinicians to choose proper centrifugation forces based on their needs.

Clinical relevance

The present findings provide theoretical basis for clinical choice of liquid H-PRF protocol from mechanical, cell contents, and histological aspects.

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Funding

The study was supported by the National Natural Science Foundation of China (82101019).

Author information

Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China

    Mengge Feng, Yan Wei, Hongjiang Wei, Yunxiao Wang, Yufeng Zhang & Yulan Wang

  2. Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China

    Yufeng Zhang

  3. Department of Periodontology, University of Bern, Bern, Switzerland

    Richard J. Miron

Authors
  1. Mengge Feng
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  2. Yan Wei
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  3. Hongjiang Wei
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  4. Yunxiao Wang
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  5. Yufeng Zhang
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  6. Richard J. Miron
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  7. Yulan Wang
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Contributions

All authors contributed to the study conception and design. The study was conducted under the supervision of Yulan Wang and Yufeng Zhang. Material preparation, data collection, and analysis were performed by Mengge Feng, Yan Wei, Hongjiang Wei, and Yunxiao Wang. The first draft of the manuscript was written by Mengge Feng, revised by Yulan Wang and Richard J Miron. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yulan Wang.

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Ethical approval

The research involves collecting blood from human participants. All protocols used were approved by the Ethics Committee of the School and Hospital of Stomatology, Wuhan University (B52/2020) in this study.

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Written informed consents were obtained from volunteers.

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Feng, M., Wei, Y., Wei, H. et al. Effect of relative centrifugal force on the biological properties of liquid platelet-rich fibrin produced via horizontal centrifugation. Clin Oral Invest 27, 399–409 (2023). https://doi.org/10.1007/s00784-022-04745-x

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  • DOI: https://doi.org/10.1007/s00784-022-04745-x

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