Simultaneous determination of l-tryptophan impurities in meat products

L-tryptophan has been used as a feed additive for swine and poultry and as a nutrient supplement for humans. However, some impurities in l-tryptophan have been reported as causative components of eosinophilia-myalgia syndrome. Therefore, from a safety perspective, it is important to analyze meat samples for these impurities. This study aims to develop an analytical method for the simultaneous detection of l-tryptophan impurities in meat products using LC–MS/MS. Among the various impurities, detection methods for (S)-2-amino-3-(5-hydroxy-1H-indol-3-yl)propanoic acid (5-hydroxytryptophan) (HTP), 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MTCA), 3a-hydroxy-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]-indole-2-carboxylic acid (PIC), and 1,1′-ethylidenebistryptophan (EBT) and 2-(3-indoylmethyl)-l-tryptophan (IMT) were developed. The developed method allowed simultaneous determination of these four impurities in 5 min. No interferences from the matrix were observed, and the method showed good sensitivity to each analyte. The method detection limit and limit of quantification in meat matrices were below 11.2 and 35.7 μg/kg, respectively.


Introduction
L-tryptophan ((S)-2-amino-3-(3-indolyl)propionic acid) is an essential amino acid in animal diets and is regarded as the fourth and third limiting amino acid for the growth of swine and poultry, respectively (Jansman et al. 2010;Peganova et al. 2003;Le Floc'h and Seve 2007). l-tryptophan is primarily used as a building block for protein synthesis. In addition to its effect on protein synthesis, l-tryptophan plays a role in other physiological processes that are associated Handling editor: D. Tsikas.
Doo-Hee Lee and Yang Hee Kim have contributed equally to this work.

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with immune and inflammatory responses such as the synthesis of serotonin and niacin (Le Floc'h and Seve 2007;Sève 1999;Höglund et al. 2019;Fukuwatari and Shibata 2013).
Methods for the analysis of l-tryptophan impurities, more specifically EMS-related substances, have been reported, and most are HPLC-based analyses. Simat et al. reported that the existence of these substances was analyzed from the pharmaceutical and feed grade of l-tryptophan by using HPLC (Simat et al. 2003). They provided the analytical method for MTCA, EBT, and IMT by using HPLC with a UV detector, of PAA by using HPLC with an amperometric detector, and PIC by using HPLC with a fluorescence detector. The detection limit of the method was between 0.5 and 7 mg/kg. Another research group reported an HPLC analysis method for the detection of MTCA, EBT, and PAA by using UV and fluorescence detection after separating the analyte with different cartridges (PRS, SCX, C8) (Adachi et al. 2000). Recently, the EBT analysis method from dietary supplements containing l-tryptophan was reported based on LC-MS/MS (Karakawa et al. 2022).
The l-tryptophan impurities in feed-grade l-tryptophan lead to human consumption through meat products. Therefore, the potential human intake of l-tryptophan impurities by consuming meat products should be considered. In this study, a simultaneous analytical method of l-tryptophan impurities in meat products was developed using LC-MS/ MS that showed higher sensitivity than the previously reported methods.
All reagents used in the analysis were of LC-MS grade and used without further purification. Ammonium formate (≥ 99% purity) and formic acid (≥ 98% purity) were procured from Sigma Aldrich (St. Louis, USA). Methanol (100% purity) and water were procured from Merck (Darmstadt, Germany).
Meat samples (pig and chicken muscle) originating from Korea, Spain, and United States were collected from a local supermarket in Korea. And other meat samples (pig muscle, skin and organs) were obtained from a slaughterhouse in Korea. The samples were stored at − 20 °C until analysis.

Sample preparation
The samples of pig muscle, pig skin, pig liver, pig kidney, pig lung, pig serum, and chicken muscle used for the spiking test and the commercial meat product were homogenized by grinding using a blender.
To obtain spiked samples, an appropriate amount of each standard chemical was added to the homogenized meat samples. The homogenized samples (1 g) were placed into a conical tube. Then, 80% methanol (10 mL) was added to the tube. The mixture was shaken and sonicated for 1 h. After sonication, the samples were centrifuged at 15,000 rpm for 10 min. The supernatants were taken and filtered using a 0.2 μm PTFE syringe filter.
A TSQ Altis triple quadrupole mass spectrometer (Thermo Scientific, USA) equipped with a heated electrospray ionization (H-ESI) ion source was used. This instrument was coupled to the UHPLC system. The source parameters of optimized mass spectrometry were positive ion 3500 V, sheath gas 50 Arb, aux gas 10 Arb, sweep gas 1 Arb, ion transfer tube temperature 325 °C, and vaporizer temperature 350 °C. Trace Finder 4.1 software (Thermo Scientific, USA) was used for the data analysis. Selected reaction monitoring (SRM) transitions were monitored to quantify the analytes. The SRM parameters are described in Table 1.

Method performance evaluation
The analytical method developed in this study was validated by referring to official guidance (ICH 2005;EC 2002EC , 2021. The performance of the analytical method was evaluated considering the specificity, method detection limit (MDL), limit of quantification (LOQ), interday precision, recovery, and matrix effect.
The linearity of the method was evaluated with calibration curves prepared in matrices by spiking analytes in pig muscle, pig skin, pig liver, pig kidney, pig lung, pig serum, and chicken muscle. The regression equation and coefficient of each calibration curve were calculated. The method detection limit and limit of quantitation were determined by analyzing seven samples with concentrations near the expected limit of detection. The standard deviation of seven samples was simply multiplied by the correct Student's t value. The t value for six degrees of freedom and a 99% confidence level is 3.14. Therefore, the MDL and LOQ were calculated to be 3.14 × sd (standard deviation) and 10 × sd (standard deviation), respectively. The interday precision was determined using a meat matrix spiked with analytes at the levels of 20 ng/mL. It was determined by analyzing the sample on seven consecutive days.
Recovery was evaluated at three fortified levels of analytes within the linear range of the calibration curve. The percentage of recovery was calculated as the concentration determined by the following equation: The matrix effects of the analysis were assessed by comparing the peak area of the standards in solvent and matrix. It was evaluated according to the following equation: Recovery (%) =100 × measured concentration ∕fortified concentration.

Specificity
The chromatogram of the compounds is shown in Fig. 1. No significant interfering peak in the LC-MS/MS chromatogram was observed under the developed analysis conditions. Individual peaks for each analyte were separated well with a run time of less than 5 min.

Linearity
The linearity of the analysis was observed through the calibration curves of each analyte obtained using the matrices spiked with the analytes. Linear regression analysis was conducted by plotting the peak area and concentration of the analyte. The linear range of the method is summarized Matrix effect (%) = 100 × peak area (matrix)∕ peak area (solvent) − 100.  Table 2. The parameters of calibration for each analyte showed very good linearity, with a mean regression coefficient (R 2 ) above 0.99.

Method detection limit and limit of quantification
The matrix-matched standard solutions of HTP, PIC, EBT, IMT, and MTCA were prepared by adding appropriate amounts of each compound to various meat matrices. The method detection limit (MDL) and limit of quantification (LOQ) of five analytes were calculated based on the seven samples of concentrations near the expected MDL (5 ng/ mL). The MDL and LOQ of the analytes are summarized in Table 2. The MDL and LOQ of this analytical method were lower than 11.2 and 35.7 μg/kg, respectively. Although the analytical method developed to detect l-tryptophan impurities in meat products, it showed a lower detection limit than the previous data in less complex matrices such as a finished product of l-tryptophan and dietary supplement (Adachi et al. 2000;Simat et al. 2003;Karakawa et al. 2022).

Accuracy and precision
The accuracy and precision of the method were assessed by a recovery test conducted by analyzing the known quantities of each compound added to the matrix-matched sample. As    Table 3, the accuracy was expressed by the recovery percentage, and precision was expressed by the relative standard deviation (% RSD).
The matrix-matched sample was spiked with HTP, PIC, EBT, IMT, and MTCA at 4 different concentrations (5, 10, 20, 50 ng/mL). Table 3 presents the recovery and RSD of the test. The recovery of the analysis ranged from 92.6 to 112.7% for pig muscle, from 97.4 to 110.2% for pig skin, from 90.8 to 108.8% for pig liver, from 89.3 to 115.9% for pig kidney, from 90.8 to 109.9% for pig lung, from 98.8 to 105.1% for pig serum, and from 94.8 to 110.2% for chicken muscle.
For the interday precision, the test solution with the same concentration (20 ng/mL) was analyzed on seven consecutive days ( Table 4). The interday precision ranged from 1.29 to 3.35% for pig muscle, from 1.32 to 3.77% for pig skin, from 2.26 to 4.55% for pig liver, from 1.65 to 4.44% for pig kidney, from 2.46 to 6.35% for pig lung, from 0.70 to 1.20% for pig serum, and from 1.39 to 4.96% for chicken muscle.
Generally, the acceptable range of mean recoveries at the relevant concentration level should be 70-120% and the precision ≤ 20% RSD (EC 2021). For all other matrices, the range of mean recoveries at the fortified concentration level was 80-120%, and the precision was less than 10% RSD. Therefore, it was clear that the accuracy and precision of the proposed method for determining l-tryptophan impurities in the meat products were within the expected ranges indicating the high accuracy and precision of the method.

Matrix effects
Matrix effect has been known as a major concern in quantitative LC-MS analysis because it affects the accuracy precision, and sensitive of the method (Zhou et al. 2017). Especially, a high concentration of nonvolatile materials solute in an extracted sample from the biological products caused the ionization suppression when ESI was used as an ionization technique (King et al. 2000). Due to the complexity of the meat matrix, the matrix effect of the developed method was evaluated by comparing the peak area of the solvent and the matrix-matched standard. The calculated matrix effects for each of the analytes in the meat matrices were summarized in Fig. 2. The percentage of the matrix effect ranged from − 17.4 to 4.7 for pig muscle, from − 8.1 to 9.7 for pig skin, from − 13.8 to 28.7 for pig liver, from − 2.8 to 23.4 for pig kidney, from − 19.0 to 4.6 for pig lung, from − 15.0 to 7.8 for pig serum, and from − 25.9 to 13.5 for chicken muscle.
Overall, the matrix effects ranged from − 20 to + 20%, which could be considered to be not significant (EC 2021), with the exception of MTCA in pig liver, IMT in pig kidney, and EBT in chicken muscle, for which the matrix effect ranged from − 25.9 to 28.7%. Without complicated purification to remove biological compound during the sample Table 4 Recovery and relative standard deviation (RSD) of the l-tryptophan impurities in meat matrices at a fortified level of 20 ng/mL (interday precision) Compound  preparation step, the matrix effect of the developed method was an acceptable range in most cases.

Application
The applicability of the developed method was evaluated by analyzing the l-tryptophan impurities in different meat products. Several kinds of commercially available meat products sold in a local supermarket and slaughterhouse in Korea were analyzed by using LC-MS/MS under the developed analytical method. As shown in Table 5, HTP, IMT and EBT were not detected in any sample tested. PIC and MTCA were detected in some of the samples. A very small amount of MTCA was detected in pig liver and kidney. PIC was detected in most of the samples, and relatively high levels of PIC were detected in organs such as the liver, kidney, and lung.
Up to now, no analytical report regarding the l-tryptophan impurities that are related to EMS in meat products as well as food or feed product has been published. Developed method in this study is the first study that analyzes l-tryptophan impurities in the biological matrices. Furthermore, the analytical result indicated that the proposed method was effective and feasible to detect l-tryptophan impurities in meat products. However, there was no information available on how much l-tryptophan and l-tryptophan-derived impurities have been fed to pigs and chickens. Therefore, a further study is necessary to observe the residue of l-tryptophan impurities in meat products after feeding a known amount of l-tryptophan and l-tryptophan impurities to an animal. Fig. 2 Matrix effects of the method for l-tryptophan impurities in seven food matrices (pig muscle, pig skin, pig liver, pig kidney, pig lung, pig serum, and chicken muscle). It was assessed by comparing the peak area of each standard in solvent and matrix

Conclusion
The safety of l-tryptophan has been under intense debate for several decades. The outbreak of EMS in 1989 was first considered to be a direct effect of l-tryptophan but was later determined to originate from impurities in the batches produced by a particular manufacturer. The causative substances of EMS-associated l-tryptophan were identified by several researchers. In this study, we developed an advanced analytical method for the detection of EMS-related l-tryptophan impurities in meat products. Although the analytical method was developed to detect l-tryptophan impurities in meat matrices, we provided simple, faster and higher sensitivity compared to the previously reported method that used HPLC coupled with different kinds of detectors such as UV, florescence and amperometric detectors. Therefore, this method is applicable for the analysis of l-tryptophan impurities in meat matrices and is simple, sensitive, rapid, reliable, and accurate.
Author contributions YHK and IKH contributed to the conception and design of the study; DL participated in the methodology design; DL and MB performed the experiment; DL and YHK wrote the main manuscript text; YS revised the manuscript. All authors reviewed the manuscript.

Data availability
The authors declare that the data supporting the findings of this study are available within the article.

Declarations
Conflict of interest YHK, MB, HIK, and YS are employed by CJ CheilJedang (Suwon, South Korea).
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