Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.)

Crop productivity is greatly affected by soil salinity; therefore, improvement in salinity tolerance of crops is a major goal in salt-tolerant breeding. The Salt Overly Sensitive (SOS) signal-transduction pathway plays a key role in ion homeostasis and salt tolerance in plants. Here, we report that overexpression of Arabidopsis thaliana SOS1+SOS2+SOS3 genes enhanced salt tolerance in tall fescue. The transgenic plants displayed superior growth and accumulated less Na+ and more K+ in roots after 350 mM NaCl treatment. Moreover, Na+ enflux, K+ influx, and Ca2+ influx were higher in the transgenic plants than in the wild-type plants. The activities of the enzyme superoxide dismutase, peroxidase, catalase, and proline content in the transgenic plants were significantly increased; however, the malondialdehyde content decreased in transgenic plants compared to the controls. These results suggested that co-expression of A. thaliana SOS1+SOS2+SOS3 genes enhanced the salt tolerance in transgenic tall fescue.


Introduction
Abiotic stresses, such as salinity, drought, and freezing, greatly limit plant growth, development, and productivity (Yamaguchi-Shinozaki et al. 2002;Huai et al. 2009;Jin et al. 2010). Soil salinity is one of these environmental abiotic stresses that decrease plant growth and productivity. It has been well documented that excessive soluble ions including sodium and chloride are harmful to most plants, including all major crops (Zhu 2001). The damage of salinity to crops is mainly caused by sodium ion accumulation, a high concentration of which causes water deficit and enzyme inactivity in most plant organs and cells (Zhu 2000). Sodium accumulation in cell walls can rapidly lead to osmotic stress and cell death (Munns 2002). Na + is not essential for plant growth, and under salt stress, it hinders uptake of the important mineral nutrient K + and competes for its enzyme binding sites. Maintaining a high K + and low Na + homeostasis in the cytoplasm is thus essential for plant salt tolerance. Plants prevent excessive Na + accumulation in the symplast by restricting influx, increasing efflux, and increasing vacuolar sequestration of Na + (Tester and Davenport 2003).
The SOS pathway is currently one of the most extensive studied mechanisms in controlling salt stress response in plants. The SOS pathway is responsible for ion homeostasis and salt tolerance in plants (Zhu 2003). The SOS1, SOS2, and SOS3 loci were first identified through forward genetic screens for salt-hypersensitive growth (Zhu 2002). AtSOS1 is a plasma membrane Na + /H + antiporter and mediates Na + efflux and control long-distance Na + transport from roots to shoots while protecting individual cells from Na + toxicity (Shi et al. 2000). AtSOS2 is a serine/threonine protein kinase (Liu et al. 2000). AtSOS3 responds to the Ca 2+ signal by activating a protein phosphatase or inhibiting a protein kinase (or by doing both) that regulates K + and Na + transport systems . AtSOS3 physically interacts with and activates AtSOS2 protein kinase . The AtSOS2/AtSOS3 kinase complex phosphorylates and activates the AtSOS1 protein (Quintero et al. 2002). As an essential second messenger, calcium regulates diverse cellular processes in plants. Calcineurin B-like (CBL) proteins belong to Ca 2+sensor protein families and function as sensor relays (Chen et al. 2012). Quan et al. (2007) reported that an AtSOS3 homolog CBL10 appears to be the main mediator for salt tolerance and CBL10 can regulate SOS pathway in Arabidopsis. Under salt stress, the calcium sensor SOS3 activates the kinase SOS2 that positively regulates SOS1. The SOS3 homolog SOS3-like calcium binding protein 8 (SCABP8)/calcineurin B-like 10 (CBL10) functions mainly in the shoot response to salt toxicity. CBL10 is a calcium-binding protein, and calcium enhances SOS2 kinase activity. CBL10 is hypersensitive to salt in shoot tissues and together with SOS3 functions to modulate the activity of SOS2 (Du et al. 2011). CBL10 and SOS3 are only partially redundant in their function, and each plays additional and unique roles in the plant salt stress response (Quan et al. 2007). CBL10 and SOS3 together protect shoots and roots from salt stress, while CBL10 alone partially associates to plasma membranes and recruits SOS2 (Du et al. 2011).
These transgenic reports clearly demonstrated the possibility and feasibility of breeding salt-tolerant plants using the AtSOS pathway genes and CBL10 gene.
Tall fescue (Festuca arundinacea Schreb.) is an important perennial cool-season grass grown or planted in temperate regions worldwide and is widely used for hay and pasture. Adaptation to a wide range of soil conditions, tolerance to continuous grazing, high yield persistence, and compatibility with varied management practices make it a widely used forage grass (Sleper and West 1996;Ge and Wang 2006). However, the growth of tall fescue is severely affected by the salinity soil in some traditional irrigation districts. In the past decade, it has been reported that gene transfer technique was applied to enhance salt tolerance in Tall fescue. Cao et al. (2009) reported the overexpression of transcription factor AtHDG11 in tall fescue resulted in enhanced tolerance to salt stress. Overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses (Lee et al. 2007). Overexpression vacuolar Na + /H + antiporter gene AtNHX1 confers enhanced salt tolerance to the transformed tall fescue progenies (Tian et al. 2006;Zhao et al. 2007). These previous results suggested that overexpression of one salt-tolerant gene could enhance salt tolerance to some degree in tall fescue.
Salt tolerance, like other important agronomic traits in crop plants, is complexly quantitative trait controlled by multi-genes. So far, little information is available on multi-gene cotransformation in tall fescue. Quintero et al. (2002) reported that the SOS system is reconstituted and co-expression of SOS1, SOS2, and SOS3 confers more salt tolerance than expression of one or two SOS proteins in yeast cells. However, overexpression of SOS1+SOS2+SOS3 improved only marginally salt tolerance in Arabidopsis compared to SOS1-or SOS3overexpressing Arabidopsis has been documented ). To our knowledge, there are no reports on the improvement of salt tolerance in the tall fescue using co-expression strategy of SOS pathway genes.
In the current study, we attempt to use the genes encoding SOS1, SOS2, SOS3, and CBL10 under the control of inducible promoter rd29A which were transferred into the tall fescue by Agrobacterium -mediated transformation with the aim to modify the salt tolerance in tall fescue. We demonstrated that the co-expression of the Arabidopsis SOS genes (SOS1/SOS2/SOS3) can extrude Na + and involve in K + acquisition in root of tall fescue under NaCl stress. Moreover, there is a link between Ca 2+ transport and SOS pathway (SOS1-SOS2-SOS3). Our data provide a new insight into salt tolerance using the co-expression system of Arabidopsis thaliana SOS1+SOS2+SOS3 genes in transgenic tall fescue.

Plant material and embryogenic cells of tall fescue
Mature seeds of tall fescue Arid 3 were obtained from Ningxia University Grass Research Institute, Yinchuan, Ningxia, P.R. China. The seeds were surface sterilized in 70 % ethanol for 2 min and in 0.1 % HgCl 2 for 8 min and then rinsed with sterilized water for six times. After surface sterilization, seeds were cultured on MS solid medium (Murashige and Skoog 1962) supplemented with 5 mg/l 2,4-D, 0.1 mg/l 6-BA, 30 g/l sucrose, 2 g/l gelrite, and pH 5.8 for embryonic calli induction and then kept in the dark for 3 weeks at 25°C. During the period, the buds were removed once a week to accelerate calli induction. Calli with shiny, yellowish, and compact structure were selected and subcultured on solid MS medium with 2 mg/l 2,4-D, 30 g/l sucrose, 2 g/l gelrite, and pH 5.8 for 2 weeks. The embryonic calli were used for transformation described as previously (Zhao et al. 2005).

Agrobacterium-mediated transformation and transgenic plant regeneration
The infection and co-cultivation of embryonic calli were carried out according to the protocol described (Zhao et al. 2005). The embryonic calli were immersed into A. tumefaciens cell suspensions for 15 min with gentle shaking. The infected calli were then transferred into a Petri dish containing a sheet of sterile filter paper permeated sterile water and co-cultured at 25°C in the dark for 3 days. After co-cultivation, the infected calli were rinsed three times with sterile water containing 300 mg/l cefotaxime and make calli dry with sterile filter paper. Afterward, the infected calli were placed on selection medium (MS solid medium plus 3 mg/l 2,4-dichlorophenoxyacetic acid, 500 mg/l Casein acid hydrolysate, 5 mg/l glufosinate, 300 mg/l cefotaxime) and cultured in the dark at 25°C for 4 weeks. Glufosinate-resistant calli were selected and transferred onto the same selection media. After 30 days, glufosinate-resistant calli were transferred onto the regeneration medium (MS solid medium plus 2 mg/l 6benzylaminopurine, 1 mg/l kinetin, 3 mg/l glufosinate, 300 mg/ l cefotaxime) until the buds grew at about 5-6 cm. The medium for forming the multiple shoots was half-strength MS medium containing 3 mg/l glufosinate. The rooted plants were shifted to soil and grown under greenhouse conditions.

Identification of transgenic plants
Genomic DNA of untransformed plants regenerated from calli (WT) and transgenic plants were extracted using CTAB method as previously described (Murray and Thompson 1980). The fragment of Bar, SOS1 , SOS2 , SOS3 , and CBL10 genes is 463, 700, 550, 683, and 460 bp respectively, which were detected by PCR with the following primer pairs (Electronic supplementary material, PCR products were separated on a 1 % agarose gel.

Southern blot analysis
Genomic DNA (10 μg) from PCR positive and wild-type plants was digested with EcoRI and separated on 1 % agarose gel by electrophoresis and then transferred to a nylon membrane. The membrane was hybridized with the Bar probe labeled with digoxingein (DIG) according to the instruction of DIG High Prime DNA Labeling and Detection Starter Kit (Roche, Switzerland). The insertion copy number of the transgene was detected on autoradiography film.

RT-PCR analysis
The WT and transgenic lines treated with different concentration of NaCl (150, 250, and 350 mM) for 7 days were harvested. The total RNA extraction was performed in Trizol kit (Invitrogen, USA). First Strand cDNA Synthesis was produced with 2 μg total RNA samples using cDNA synthesis supermix (Transgen, China) .The expressions of Bar and SOS1 gene were amplified with reverse transcription PCR (RT-PCR). The primers sequence of Bar and SOS1 were described previously. Amplified fragments were separated on 1 % agarose gel.

Glufosinate resistance
To identify the glufosinate resistance of transgenic plants, 250 mg/l glufosinate were sprayed on the leaves of the WT and T 1 plants of transgenic lines 6-2 and observed the phenotype of plants.

Salt stress treatment
The WT and T 1 generation of transgenic plants were grown in plastic pots filled with a peat moss, vermiculite mixture located in a greenhouse. The plants were treated with nutrient  TM2, CBL10, TM1, SOS2, SOS3, SOS1, TM220,   TM1, TM2, and TM220 are the MAR sequences, can overcome transgenic silencing, and enhance the expression levels of the transgenes solution containing 0, 150, 250, and 350 mM NaCl for 7 days, respectively. The solutions were replaced everyday. The leaves and roots were collected, and the contents and flux of Na + and K + ; the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT); and the contents of malondialdehyde (MDA) and proline (Pro) were measured, respectively, after 350 mM NaCl treatment.

Determination of Na + and K + contents
The leaves were collected and dehydrated in an oven at 80°C for 2 days. The ash samples were digested with HNO 3 overnight, and the contents of Na + and K + were measured by atomic absorption spectrophotometry (Xu et al. 2006).

Ion flux assays
Net flux of K + , Na + , and Ca 2+ were measured using Noninvasive Micro-test Technique. The scanning ion-selective electrode technique (SIET) using the SIET system (BIO-001B, Younger USA Sci. &Tech. Corp, Amherst, MA, USA). Microelectrodes were filled with different cocktails by Xuyue Science and Technology Cooperation Limited as described previously (Yue et al. 2012). Net ion flux were measured from the root epidermis in mature (2-3 mm from the root tip) and meristematic (120 μm from the tip) zones. During measurements, the software controlled the PatchMan NP2 to move the electrodes between two positions, 50 and 350 μm from the root surface in a 10-s square-wave manner. The software also recorded electric potential differences from the electrodes between the two positions using a DAS08 analogue to digital card (Computer Boards, USA) in the computer.
Enzyme activity measurements of SOD, POD, and CAT and content detection of MDA and Pro Pro content was measured according to Bates method (Bates et al. 1973). SOD activity was measured according to Beauchamp and Fridovich method (Beauchamp and Fridovich 1971). CAT activity was determined as described by Beers and Sizers method (Beers and Sizers 1952). Measurements of POD activity were carried out according to Gong (2001). MDA content was determined by thiobarbituric acid (TBA) test (Draper et al. 1993).

Transformation of tall fescue
After an extensive genotype screening, Arid 3, one of the most tissue culture-responsive commercial varieties, was chosen for all transformation experiments (Electronic supplementary material, Table 2). The construct was introduced into embryogenic calli via Agrobacterium-mediated transformation (Fig. 2a). The infected calli were co-cultured for 3 days (Fig. 2b). Putative transgenic calli were selected on plants containing 5 mg/l glufosinate for 4 weeks. Glufosinate-resistant calli were white or light yellow, and grew the buds, whereas non-transgenic calli gradually turned brown (Fig. 2c). Shoots emerged from the explants on media containing 2 mg/l glufosinate. After 3 weeks, putative transformed plants grew quickly and formed strong root systems (Fig. 2d). Subsequently, the transformed plants were transformed to the greenhouse for acclimatization (Fig. 2e, f).

Molecular identification of transgenic plants
To validate whether the target genes integrated into the tall fescue genome, PCR reactions were first performed with primers designed to amplify the sequence of Bar, SOS1, SOS2, SOS3, and CBL10 genes individually (Fig. 3a-e). We totally obtained 47 bar-resistant lines and further identified by PCR. The integration of the Bar gene into the Tall fescue genome was further confirmed by southern blot. The genomic DNA was digested by EcoRI and hybridized with the Bar probe. Among the six tested transgenic lines, four out of six lanes showed one to two hybridization signals, whereas no hybridization signals was detected in wild-type plants (Fig. 3f).
Expressions of the Bar gene and SOS1 gene were confirmed by RT-PCR analysis. A Bar band with the expected size of (463 bp) and an SOS1 band with the expected size of (700 bp) were amplified from transgenic lines having salt stress but nothing from wild-type plants (Fig. 3g).

Identification of glufosinate resistance
The wild-type plants could not resist the glufosinate, and the leaves became dry yellow and withered after 3 days; however, the leaves of transgenic plants maintained green and grew normal (Fig. 4).

Enhanced salt tolerance in the transgenic plants
Based on the identification of glufosinate resistance, 12 plants of T 1 generation line 6-2 and WT were selected, respectively, and treated with different concentrations of NaCl (0, 150, 250, and 350 mM) to analyze salt tolerance, and each treatment was triplicates. After 7 days, obvious phenotypic differences were observed between wild type and transgenic line 6-2. Wildtype plants showed growth retardation, whereas the transgenic plants displayed normal growth (Fig. 5).

Na + and K + accumulation in transgenic plants
To test whether overexpression of these genes can reduce Na + accumulation in plants, we compared the Na + and K + contents among the transgenic and WT plants. Na + and K + contents in leaves were determined with atomic emission spectrophotometer. Na + and K + contents were similar between transgenic and WT plants without NaCl treatment. With the 350 mM concentration of salt, the Na + concentration in leaves of transgenic and wildtype plants also increased. However, the wild-type plants accumulated more salt in leaves than transgenic plants (Fig. 6a). At 350 mM of salt stress, the wild-type plants withered after 7 days, whereas transgenic plants showed normal growth and produced new leaves.
The K + contents in the leaves of transgenic and wild-type plants increased as the NaCl concentration increased, whereas there was significant difference of K + content between leaves of wild-type and transgenic plants, the K + content of transgenic plants in leaves are more than the wild-type plants under salt treatment (Fig. 6b).
Non-invasive ion flux measurements K + efflux in WT and K + influx in transgenic plants were showed without NaCl treatment. Salinity stress caused significant changes in net ion flux, and 350 mM NaCl treatment caused a K + efflux in roots both for WT and transgenic plants (Fig. 7a). Roots of transgenic plants lost less K + instantaneously than did WT plants. NaCl treatment increased the mean rate of K + flux for both WT and transgenic plants (Fig. 7b). However, the mean rates of K + flux in transgenic plants were smaller than WT plants without or with NaCl treatments.
Na + uptake, transport, and compartmentation are crucial for plants to survive saline environments with high NaCl content. Na + flux are influx in root of transgenic plants, while Na + flux are efflux in roots of WT plants without NaCl treatment. The transgenic plants and WT plants are treated with 350 mM NaCl for 7 days, salt induced Na + efflux both in roots of transgenic plants and WT plants (Fig. 8a). The mean rates of Na + flux in transgenic plants were more than WT plants with NaCl treatments (Fig. 8b).
Ca 2+ efflux was significantly higher in the root of WT plants than in transgenic plants in normal condition. Higher Ca 2+ influx was observed in roots both for WT and transgenic plants treated with 350 mM NaCl for 7 days (Fig. 9a). However, the mean rates of Ca 2+ influx in transgenic plants increased apparently than WT plants with NaCl treatment (Fig. 9b).

Changes of physiological characteristics
The Pro content of transgenic and wild-type plants was measured before and after salt treatment. Before the salt stress, the Pro content was almost same in both types of plants. However, an obvious increase was observed in the transgenic and wild plants, which resulted in an about ninefold to fourfold higher Pro content level in the transgenic and wild plants after salt stress, and the Pro content in the leaves of transgenic plants was significantly higher than wild types (Fig. 10a).
The SOD activity change was obviously different between the transgenic and wild-type plants. Under salt stress, the SOD activity in all transgenic plants showed only a slight increase, while SOD activity in the wildtype plants increased significantly (Fig. 10b). The CAT activity was also similarly increased in the transgenic plants after salt stress; however, the CAT activity in wild-type plants reduced slightly compared to what observed before treatment (Fig. 10c). The POD activity increased both in the transgenic and wild-type plants after salt stress, while the increase in the transgenic plants was significantly higher than that of the wild-type plants (Fig. 10d). The MDA content increased in wild-type plants after salt stress; however, the MDA content decreased in transgenic plants (Fig. 10e).

Discussion
To date, the majority of studies reported are involved in the manipulation of single genes in the improvement of salt tolerance. However, salt tolerance is associated with the interactions of many genes; thus, co-overexpression strategy of multi-genes is a promising way to improve salt tolerance. In this study, SOS1 +SOS2 +SOS3 were in combination in one vector, with each gene being driven by a stress-inducible rd29A promoter, respectively. We obtained 47 transgenic lines and one transgenic line 6-2 was selected to test the salt tolerance. Our results indicated that co-expression of these three SOS pathway genes confers enhanced salt tolerance in transgenic tall fescue. a PCR amplification of 463 bp Bar gene. M, DL2000 marker, CK + , plasmid, CK − , wild-type, lanes 1,2,3, and 4 represent PCR amplicon from line 6-2, 6-3, 6-4, 6-5, and 6-6, respectively. b PCR amplification of 700 bp SOS1 gene. c PCR amplification of 550 bp SOS2 gene. d PCR amplification of 683 bp SOS3 gene. e PCR amplification of 460 bp CBL10 gene. f Analysis of transgenic tall fescue plants (lanes 1-6) by southern blot hybridization after digesting genomic. DNA with EcoI and probing with labeled Bar. M molecular marker, W wild-type plant. g RT-PCR analysis of Bar and SOS1 transcripts in transgenic plants. M , DL2000 marker, lanes 1 and 2 represent Bar amplicon from lines 6-2 and 6-3. Lane 3 represents Bar amplicon from WT. Lanes 4 and 5 represent SOS1 PCR amplicons from lines 6-2 and 6-3, respectively In addition, symptoms of major damage caused by salt stress, such as growth retardation, yellowing of old leaves, and death of older leaves, were moderated in transgenic plants (Fig. 5) as compared to WT controls. The transgenic lines 6-2 exhibited greatly improved phenotype over WT plants in salt stress, indicating that co-expression of SOS genes confers enhanced salt tolerance in tall fescue. This performance was consistent with tall fescue overexpression of OsNHX1 , a vacuolar Na + /H + antiporter ). These results further suggest the potential use of transgenic tall fescue in the co-expression of SOS genes in saline soil. Therefore, the analysis of salt tolerance in transgenic plants showed coexpression of SOS pathway genes and CBL10 gene could improve the salt tolerance of transgenic plants, which were consistent with glufosinate resistance test (Fig. 4).
The salt tolerance of plants is associated with the ability to extrude Na + from the root and maintain a low Na + /K + ratio in the cell. Transgenic plants overexpression SOS1 (driven by the 35S promoter) apparently reduced Na + accumulation (Shi et al. 2003). The Na + accumulation in transgenic plants with overexpression of SOS3, AtNHX1+SOS3, SOS2+SOS3, or SOS1+SOS2+SOS3, respectively, was reduced as compared to that in wild-type plants under salt treatment ). SOS2/SOS3 complex regulates the expression level of Fig. 6 Na + (a) and K + (b) content changes in the transgenic plants and wild-type plants in leaves under salt stress. CK wildtype plant, 6-2 transgenic line Fig. 7 Effect of salinity (350 mM NaCl) on net K + flux (influx negative) measured in roots of transgenic lines (6-2) and wildtype (WT) plants. CK-0 wild-type plant without treatment, CK-350 wild-type plants with 350 mmol NaCl treatment, 6-2-0 transgenic lines 6-2 without treatment, 6-2-350 transgenic lines 6-2 with 350 mM NaCl treatment. a K + flux for WT and transgenic plants roots. b The mean rate of K + flux during the period of salt-stress treatment a salt tolerance effector gene encoding SOS1, a plasma membrane Na + /H + antiporter, which extrudes excess Na + from the cytosol (Shi et al. 2000). The SOS signal transduction pathway has also been shown to be involved in K + acquisition by roots (Wu et al. 1996;Zhu et al. 1998). SOS1-overexpressing seedlings with 150 mM NaCl stress accumulated less Na + and more K + (Yue et al. 2012). Similar to those with the previous results, we found that SOS1 +SOS2 +SOS3 -and CBL10overexpressing transgenic plants accumulated less Na + than WT plants under salt stress (350 mM NaCl) (Figs. 6a and 8a, b). Therefore, co-expression of the Arabidopsis SOS genes (SOS1/SOS2/SOS3) and CBL10 can extrude Na + from the cytoplasm in tall fescue roots and leaves, mitigate the toxic effects of Na + , and improve salt tolerance under NaCl stress, and this may be due to the SOS pathway and CBL10 which are responsible for Na + homeostasis in plants. CBL10 and SOS3 must fulfill distinct regulatory functions in the salt stress response, as they could not replace each other in genetic complementation. Salt stress can induce SOS2 phosphorylates CBL10 at the plasma membrane, stabilizes the CBL10-SOS2 interaction, and enhances plasma membrane Na + /H + exchange activity (Lin et al., 2009). SOS3 and SOS2 are involved in sensing and responding to the influx of sodium ions. This complex activates SOS1, a plasma membrane-located sodium/proton antiporter. Activation of SOS1 mediates the export of the Na + ions (Lin et al. 2009). Therefore, co-expression SOS pathway genes and CBL10 gene could make the transgenic plants decrease accumulation of Na + and mitigate the toxic effects of Na + .
Adaptation of plants to saline environments not only depends on their ability to avert the toxic effects of Na + but also on their ability to overcome salt-induced injury, and it was closely related with K + uptake and K + homeostasis. Salinity may reduce K + uptake as a result of competition between Na + and K + for plasma membrane uptake sites (Tyerman and Skerrett 1999;Maathuis and Amtmann 1999;Tester and Davenport 2003). SOS1 protected the AKT1 of K + channel, which mediated K + influx in the presence of increased Na + (Qi and Spalding 2004;Shabala et al. 2005). Consistent with those reports, our results showed that K + efflux from the elongation region of roots of transgenic plants overexpressing SOS1+ SOS2 +SOS3 was smaller than that from WT plants (Figs. 6b and 7a, b). These may be due to the SOS signal transduction pathway which is also involved in K + acquisition in roots. We assumed that the transgenic tall fescue having lower Na + levels and higher K + levels in the leaves and roots, respectively, in salt stress, is likely because the co-expression of the SOS genes could affect K + transported by Na + levels and promote K + uptake to maintain K + homeostasis.
Ca 2+ has been widely implicated as an intracellular messenger of physiologically and environmentally induced signaling pathways in plants (Trewavas and Malho 1998). Because cellular Ca 2+ levels are tightly The mean rate of Na + flux during the period of salt-stress treatment regulated, small changes in intracellular Ca 2+ can provide information for the modification of enzyme activity and gene expression needed for subsequent responses . Salt stress elicits a transient increase of Ca 2+ that is sensed by SOS3 , a myristoylated calcium-binding protein, which interacts with and activates SOS2 , a serine/threonine protein kinase. The SOS2/SOS3 kinase complex phosphorylates and activates the SOS1 protein (Qiu et al. 2002;Zhu 2003). Kun-Mei Guo found that the SOS mutations alter the activity of Ca 2+ transport systems in normal and NaClaffected conditions, and all SOS mutations led to increased Ca 2+ influx into the meristem cells in comparison with WT in the absence of salt stress because SOS mutants are needed to increase Ca 2+ sequestration into internal cell compartments to maintain [Ca 2+ ] cyt at the WT level ). An increase in [Ca 2+ ] cyt during NaCl stress has been reported by Kiegle et al. (2000). Ca 2+ regulation of SOS3 has been observed previously (Gong et al. 2004). Based on the combination of previous studies and data presented here (Fig. 9a, b), the mean rates of Ca 2+ influx in transgenic plants increased apparently than in WT plants with 350 mM NaCl treatment. We suggested that there were links between Ca 2+ transport and SOS pathway (SOS1 -SOS2 -SOS3 ) and the regulation of Na + and Ca 2+ homeostasis in tall fescue. Earlier report demonstrated that SOS1 was apparently involved in enhancing Ca 2+ transport in NaCl-affected conditions ). The CBL10 gene was shown to bind calcium, interact with SOS2 both in vitro and in vivo, recruit SOS2 to the plasma membrane, enhance SOS2 activity in a calciumdependent manner, and activate SOS1 in yeast (Quan et al. 2007). The CBL10 gene is associated with membrane fractions. This membrane localization is consistent with the idea that many Ca 2+ -signaling events are initiated by Ca 2+ fluxes across membranes (Rudd and Franklin-Tong 1999). Moreover, calcium is widely recognized to play an important part in regulating the passive entry of Na + and in K + /Na + selectivity. Thus, it is probable that the enhanced root levels of Ca 2+ in transgenic plants could guarantee the integrity of its membranes, allowing the change in K + /Na + and the selective absorption of K + . However, there was a significant difference in K + , Na + , and Ca 2+ flux between the WT and transgenic plants under non-salt treatment. This would be the reason of difference in root zone at the same distance (500 μm) between them.
Salinity could generate ion toxicity to cell, which caused reactive oxygen species (ROS) and proline accumulation Fig. 9 Effect of salinity (350 mM NaCl) on net Ca + flux (influx negative) measured in roots of transgenic lines (6-2) and wildtype (WT) plants. CK-0 WT plants without treatment, CK-350 WT plants with 350 mmol NaCl treatment, 6-2-0 transgenic lines 6-2 without treatment, 6-2-350 transgenic lines 6-2 with 350 mmol NaCl treatment. a Ca 2+ flux for WT and transgenic plants in roots. b The mean rate of Ca + flux during the period of saltstress treatment in plants (Greenway and Munns 1980;Xiong et al. 2002). The ROS accumulation leads to the instability of the cell membrane system and inhibits plant growth and development (Li et al. 2011).The SOD, POD, and CAT are the important antioxidation enzymes for decreasing ROS harm and maintaining the integrity of the cellular membrane construction (Li et al. 2011). In this report, we assayed the changes of physiological characteristics including SOD, POD, and CAT activity and content of MDA and Pro. In transgenic tall fescue, the Na + could not generate serious ion toxicity to cell and the excessive Na + induced SOD, POD, and CAT activity to decrease ROS accumulation (Figs. 6a and 10b-d). However, ROS might not be scavenged by SOD, POD, and CAT instantly and effectively, which caused the significant rise of the Pro content. This might have been more severe with higher Na + contents of the cell with 350 mM NaCl treatments. For WT, without SOS pathway genes and CBL gene, the absorbed Na + could not be efficiently mediated by Na + efflux to maintain a sufficiently low Na + concentration in cytoplasm; therefore, the higher Na + contents induced SOD, POD, and CAT activity and Pro content were inferior to transgenic tall fescue. These results implied that the Na + content of cytoplasm in transgenic tall fescue could be kept at a low level and the SOS pathway genes and CBL10 gene accelerated a large amount of Na + efflux. Consequently, the co-expression of SOS pathway genes and CBL10 gene could efficiently maintain Na + homeostasis of cytoplasm and improve salt tolerance of transgenic plants.
The MDA content of WT showed a significant increase in comparison to transgenic plants with 350 mM NaCl treatment (Fig. 10e). The increased MDA content might be due to the membrane destruction in WT resulting from ROSinduced oxidative damage ). The salt treatment may be involved in scavenging ROS more efficiently in the transgenic plants. Proline accumulation may be the symptom of injury caused or the adaptive response in salt stress (Fig. 10a) (Riazi et al. 1985;Aspinall and Paleg 1981). Osmoregulation has been attributed to Pro accumulation in tissues of the plants in response to salt stress (De and Maiti 1995). Moreover, Pro would stabilize enzymes as RUBISCO, allowing its efficient functioning even in the presence of NaCl (Solomon et al. 1994).
In summary, we obtained 47 transgenic tall fescue lines via Agrobacterium-mediated transformation. Moreover, a typical line 6-2 was further used to investigate its salt tolerance. Results from the contents and flux of Na + , K + , and Ca 2+ and the physiological traits illustrated that the SOS1 +SOS2 + SOS3 -overexpressing transgenic tall fescue had enhanced tolerance to salt stress. Our result may provide a new insight in molecular breeding of turfgrass and be helpful in exploiting and utilizing saline soil.