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Complete genome sequence of a novel virus belonging to the genus Badnavirus in jujube (Ziziphus jujuba Mill.) in China

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Abstract

The genome sequence of a novel circular DNA virus related to members of the genus Badnavirus was identified in diseased jujube trees by high-throughput sequencing and verified by conventional Sanger sequencing of cloned PCR products. The name "jujube badnavirus WS" (JuBWS) is proposed for this virus. Diseased jujube leaves showed yellow mosaic and malformation symptoms, with round chlorotic spots found on diseased fruit. The genome of this virus has a length of 6450 nt and has a typical badnavirus genomic structure with three open reading frames (ORFs). JuBWS was identified as a novel badnavirus based on nucleotide differences in the RNase (RT + RNase H) coding region of ORF3. The JuBWS sequence showed 70.48–76.41% nucleotide sequence identity to other known badnaviruses, thus meeting the taxonomic criterion for establishing a new species within the genus Badnavirus. This study suggested that the novel badnavirus might be a pathogen associated with jujube mosaic disease, and this will be investigated in the future.

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Acknowledgements

Financial support was provided by the Research and Demonstration of Key Technologies for Blockade Prevention and Control of Jujube Virus Diseases in Xinjiang (grant number: 2018E02026) and “Excellent Postdoctoral Professionals and General Funding Staff of Living Subsidies” from the Department of Human Resources and Social Security of Xinjiang Uygur Autonomous Region.

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Author notes

  1. Baojun Liu and Guoxin Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China

    Baojun Liu, Guoxin Zhang, Danbo Song, Quan Wang & Aixing Gu

  2. Institute of Economic Forestry, Xinjiang Academy of Forestry Sciences, Urumqi, 830063, China

    Baojun Liu, Hong Li & Jianyu Bai

Authors
  1. Baojun Liu
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  2. Guoxin Zhang
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  3. Danbo Song
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  4. Quan Wang
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  5. Hong Li
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  6. Aixing Gu
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  7. Jianyu Bai
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Correspondence to Hong Li, Aixing Gu or Jianyu Bai.

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Liu, B., Zhang, G., Song, D. et al. Complete genome sequence of a novel virus belonging to the genus Badnavirus in jujube (Ziziphus jujuba Mill.) in China. Arch Virol 167, 1885–1888 (2022). https://doi.org/10.1007/s00705-022-05482-y

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  • DOI: https://doi.org/10.1007/s00705-022-05482-y

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