Skip to main content
SpringerLink
Log in

Identification and molecular characterization of a novel kudzu-infecting virus of the family Betaflexiviridae

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A new virus, tentatively named “kudzu virus D” (KuVD) was discovered in kudzu (Pueraria montana var. lobata) in South Korea. Its complete genome comprises 7,922 nucleotides, excluding the poly(A) tail, and contains five open reading frames (ORFs) encoding, from 5′ to 3′, a replicase (ORF1), three triple gene block proteins TGB1–3 (ORF2–ORF4), and a coat protein (ORF5). This genome organization is typical of members of the subfamily Quinvirinae of the family Betaflexiviridae. Pairwise alignment analysis revealed that the nucleotide sequences of the replicase and coat protein of KuVD were 12.13-54.46% and 24.03-50.67% identical, respectively, to those of other members of the family Betaflexiviridae. These values are far below the current species ICTV demarcation threshold. Consequently, KuVD should be considered a member of a new species in the subfamily Quinvirinae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Tungmunnithum D, Intharuksa A, Sasaki Y (2020) A Promising View of Kudzu Plant, Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep: flavonoid phytochemical compounds, taxonomic data, traditional uses and potential biological activities for future cosmetic application. Cosmetics 7:12

    Article  CAS  Google Scholar 

  2. Zhang J, Wu Z (2013) First Report of Kudzu mosaic virus on Pueraria montana (kudzu) in China. Plant Dis 97:148–148

    Article  CAS  Google Scholar 

  3. Khankhum S, Bollich P, Valverde R (2013) First report of tobacco ringspot virus infecting kudzu (Pueraria montana) in Louisiana. Plant Dis 97:561–561

    Article  CAS  Google Scholar 

  4. Zhou J, Aboughanem-Sabanadzovic N, Sabanadzovic S, Tzanetakis IE (2018) First report of Soybean vein necrosis virus infecting Kudzu (Pueraria montana) in the United States of America. Plant Dis 102:1674–1674

    Article  Google Scholar 

  5. Adams MJ, Antoniw JF, Bar-Joseph M, Brunt AA, Candresse T, Foster GD, Martelli GP, Milne RG, Zavriev SK, Fauquet CM (2004) The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149:1045–1060

    Article  CAS  Google Scholar 

  6. Xin M, Zhang P, Liu W, Ren Y, Cao M, Wang X (2017) The complete nucleotide sequence and genome organization of a novel betaflexivirus infecting Citrullus lanatus. Arch Virol 162:3239–3242

    Article  CAS  Google Scholar 

  7. Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2011) Family Betaflexiviridae. In: King AMQ, Adams MJ, Carstend EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the International Committee on taxonomy of viruses. Elsevier, Academic Press, Amsterdam, pp 920–941

    Google Scholar 

  8. Lefkowitz EJ, Dempsey DM, Hendrickson RC, Orton RJ, Siddell SG, Smith DB (2018) Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic Acids Res 46:D708-d717

    Article  CAS  Google Scholar 

  9. Melcher U (2000) The “30K” superfamily of viral movement proteins. J Gen Virol 81:257–266

    CAS  PubMed  Google Scholar 

  10. Morozov SY, Solovyev AG (2003) Triple gene block: modular design of a multifunctional machine for plant virus movement. J Gen Virol 841:1351–1366

    Article  Google Scholar 

  11. Adams MJ, Lefkowitz EJ, King AMQ, Harrach B, Harrison RL, Knowles NJ, Kropinski AM, Krupovic M, Kuhn JH, Mushegian AR, Nibert M, Sabanadzovic S, Sanfaçon H, Siddell SG, Simmonds P, Varsani A, Zerbini FM, Gorbalenya AE, Davison AJ (2016) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016). Arch Virol 161:2921–2949

    Article  CAS  Google Scholar 

  12. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries; Project No. AGC1762111), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. This study was also supported by the Animal and Plant Quarantine Agency (No. FDM0012211) Republic of Korea. We thank Jennifer Smith, Ph.D, from Edanz (http://www.edanz.com/ac) for editing a draft of this manuscript.

Author information

Authors and Affiliations

  1. Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia

    Davaajargal Igori

  2. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Davaajargal Igori, Ah-Young Shin, Se-Eun Kim, Suk‑Yoon Kwon & Jae Sun Moon

  3. Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

    Suk‑Yoon Kwon & Jae Sun Moon

Authors
  1. Davaajargal Igori
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ah-Young Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Se-Eun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Suk‑Yoon Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jae Sun Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Suk‑Yoon Kwon or Jae Sun Moon.

Additional information

Handling Editor: Jesús Navas-Castillo.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (TXT 8 KB)

Supplementary file2 (DOCX 29 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Igori, D., Shin, AY., Kim, SE. et al. Identification and molecular characterization of a novel kudzu-infecting virus of the family Betaflexiviridae. Arch Virol 167, 1707–1711 (2022). https://doi.org/10.1007/s00705-022-05468-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-022-05468-w

Search

Navigation