Abstract
The complete genome sequence of peony leafroll-associated virus (PLRaV) was determined by deep sequencing of ribosomal-RNA-depleted total RNA extracted from a peony plant exhibiting leafroll symptoms. Further PCR and RACE analysis showed that the PLRaV genome consists of 15,406 nucleotides and contains 10 putative open reading frames, with an organization typical of members of the genus Ampelovirus, family Closteroviridae. Amino acid sequence comparisons showed that the viral heat shock protein 70 homolog (HSP70h) shared the highest sequence identity (41.7%) with the corresponding region of grapevine leafroll-associated virus 1, and the coat protein (CP) and RNA-dependent RNA polymerase (RdRp) shared the highest sequence identity (32.1% and 52.3%, respectively) with grapevine leafroll-associated virus 13. Phylogenetic analysis of the HSP70h, CP, and RdRp aa sequences showed that PLRaV clustered with members of subgroup I of the genus Ampelovirus.
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References
Bremer K (1985) Strawberry latent ringspot virus in ornamental plants in Finland. Ann Agric Fenn 24:101–102
Wei NS, Wu YF (1990) On a peony isolate of Tobacco rattle virus (TRV-Pa). Acta Phytopathol Sin 20:247–251
Bellardi MG, Rubies-Autonell C, Bianchi A (2003) First report of a disease of peony caused by alfalfa mosaic virus. Plant Dis 87:99
Parrella G, Gognalons P, Gebre-Selassiè K, Volvas C, Marchoux G (2003) An update of the host range of Tomato spotted wilt virus. J Plant Pathol 85:227–264
Cardin L, Onesto JP, Moury B (2010) First report of Cucumber mosaic virus in Paeonia lactifera in France. Plant Dis 94:790
Gress JC, Smith S, Tzanetakis IE (2017) First report of Citrus leaf blotch virus in peony in the USA. Plant Dis 101:637
Shaffer C, Gress JC, Tzanetakis IE (2019) First report of Cycas necrotic stunt virus and Lychnis mottle virus in peony in the United States. Plant Dis 103:1048
Shaffer C, Michener DC, Viasava N, Botermans M, Starre J, Tzanetakis IE (2021) First report of gentian Kobu-sho-associated virus infecting peony in the United States and the Netherlands. Plant Dis. https://doi.org/10.1094/PDIS-06-21-1316-PDN
Shaffer C, Vakić M, Tzanetakis IE (2021) First report of amazon lily mild mottle virus in peony in the United States. Plant Dis 105:236
Jia AN, Yan CG, Yin H, Sun R, Xia F, Gao L, Li YQ, Zhang YJ (2021) Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses. Plant Dis. https://doi.org/10.1094/PDIS-01-21-0007-RE
Chen YX, Chen YS, Shi CM, Huang ZB, Zhang Y, Li SK, Li Y, Ye J, Yu C, Li Z, Zhang XQ, Wang J, Yang HM, Chen Q (2018) SOAPnuke: a MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughputsequencing data. Gigascience 7:1–6
Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644–652
Agranovsky AA, Koonin EV, Boyko VP, Maiss E, Frotschl R, Lunina NA, Atabekov JG (1994) Beet yellows closterovirus: complete genomestructure and identification of a leader papain-like thiol protease. Virol 198:311–324
Karasev AV, Boyko VP, Gowda S, Nikolaeva OV, Hilf ME, Koonin EV, Niblett CL, Cline K, Gumpf DJ, Lee RF, Garnsey SM, Lewandowski DJ, Dawson WO (1995) Complete sequence of the citrustristeza virus RNA genome. Virol 208:511–520
Karasev AV, Nikolaeva OV, Koonin EV, Gumpf DJ, Garnsey SM (1994) Screening of the closterovirus genome by degenerate primer-mediated polymerase chain reaction. J Gen Virol 75:1415–1422
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0.Mol. Biol Evol 30:2725–2729
Fuchs M, Bar-Joseph M, Candresse T, Maree HJ, Martelli GP, Melzer MJ, Menzel W, Minafra A, Sabanadzovic S, ICTV Report Consortium (2020) ICTV virus taxonomy profile: Closteroviridae. J Gen Virol 101:364–365
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This work was supported by a Grant from the Beijing Municipal Education Committee (CIT&TCD201904055).
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Dong, Z., Liang, Y., Wang, X. et al. Complete genome sequence of peony leafroll-associated virus, a novel ampelovirus in subgroup I. Arch Virol 167, 995–998 (2022). https://doi.org/10.1007/s00705-022-05389-8
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DOI: https://doi.org/10.1007/s00705-022-05389-8