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CTAB-Co-MOFs@AuPt NPs as signal probes for the electrochemical detection of carcinoembryonic antigen 15–3

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Abstract

A sensitive electrochemical strategy for carcinoembryonic antigen 15–3 (CA15-3) detection is reported using CTAB-Co-MOFs@AuPt NPs as signal probes. The electrochemical strategy was designed as follows: First, the graphene aerogel@gold nanoparticles (GA@Au NPs) nanocomposites were employed to modify the sensing surface for promoting electron transfer rate and primary antibody (Ab1) immobilization due to GA possesses a large specific surface area, eminent conductivity, and a 3D network structure. Cobalt metal–organic frameworks (CTAB-Co-MOFs) synthesized were then used as a carrier for AuPt NPs and secondary antibody (Ab2) immobilization (notes: labelled-Ab2). With sandwich immunoreaction, the labelled-Ab2 was captured on the surface of the GA@Au NPs nanocomposites. Finally, differential pulse voltammetry (DPV) was employed to register the electrochemical signal of the immunosensor at the potential of − 0.85 V (vs SCE) in phosphate buffer saline (PBS) containing 2.5 mM H2O2. It was verified that the electrochemical reduction signal from Co3+ to Co2+ was recorded. The AuPt NPs could catalyze the reaction of H2O2 oxidizing Co2+ to Co3+, resulting in the amplification of the electrochemical signal. Under the selected conditions, the immunosensor can detect CA15-3 in the range 10 µU/mL to 250 U/mL with a low detection limit of 1.1 µU/mL. In the designed strategy, the CTAB-Co-MOFs were not only employed as carriers for AuPt NPs, but also acted as signal probes. The CTAB-Co-MOFs were investigated including SEM, TEM, XPS, and XRD. The application ability of the immunosensor was evaluated using serum sample, demonstrating the immunosensor can be applied to clinic serum analysis.

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Funding

This work is supported by the Nature Science Foundation of the Education Department of Anhui Province (No KJ2016A848).

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chem-Biosensing, Anhui Normal University, Wuhu, 241002, People’s Republic of China

    Tingting Zhang, Yan Ma, Ruifen Tian & Yuzhong Zhang

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  1. Tingting Zhang
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  2. Yan Ma
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Correspondence to Yuzhong Zhang.

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All experiments were performed in accordance with the relevant laws and institutional guidelines of China, and approved by the ethics committee at Anhui Normal University (AHNU-ET2023051).

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Zhang, T., Ma, Y., Tian, R. et al. CTAB-Co-MOFs@AuPt NPs as signal probes for the electrochemical detection of carcinoembryonic antigen 15–3. Microchim Acta 191, 176 (2024). https://doi.org/10.1007/s00604-024-06254-y

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