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Bioenzyme-free colorimetric assay for creatinine determination based on Mn3O4 nanoparticles catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine

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Abstract

Mn3O4 nanozyme with good oxidase-like activity was successfully synthesized. The prepared Mn3O4 nanozyme can directly and effectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate green–blue-colored ox-TMB. Creatinine exhibits distinct inhibition effect on Mn3O4 nanozyme-catalyzed TMB colorimetric reaction system, leading to obvious color fading and absorbance intensity decrease of the reaction system. Furthermore, interference from uric acid can be effectively eliminated by regulating the pH of TMB-Mn3O4 colorimetric reaction system to pH 2.0. Then, a simple and bioenzyme-free colorimetric assay for the determination of creatinine was developed based on TMB-Mn3O4 colorimetric reaction. The linear detection range is from 100 to 800 μM and from 1 to 20 mM. The lowest limit of detection is 35.3 μM. Satisfied results are obtained for the determination of creatinine in real urine and sweat samples. This work provides the synthesis of a good oxidase-like nanozyme Mn3O4 and presents the fabrication of an effective nanozyme-based bioenzyme-free colorimetric assay for the determination of creatinine.

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Acknowledgements

This work is supported by NSAF (Grant No. U2230117), the Fundamental Research Funds for the Central Universities (PA2022GDSK0044) and the Undergraduate Innovation and Entrepreneurship Training Program (X202310359291).

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Authors and Affiliations

  1. Anhui Province Key Laboratory of Advanced Catalytic Materials and Reaction Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology, Hefei, Anhui, 230009, People’s Republic of China

    Yuyang Lu, Nuotong Shen, Yachao Xi, Tao Zhu, Hao Peng, Lihao Zhong & Fang Li

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  1. Yuyang Lu
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  2. Nuotong Shen
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  4. Tao Zhu
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  7. Fang Li
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Correspondence to Fang Li.

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The experimental protocol was approved by the Research Ethics committee of Hefei University of Technology, China (Project No. U2230117).

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Lu, Y., Shen, N., Xi, Y. et al. Bioenzyme-free colorimetric assay for creatinine determination based on Mn3O4 nanoparticles catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine. Microchim Acta 191, 44 (2024). https://doi.org/10.1007/s00604-023-06129-8

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