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A MnO2 nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots and o-phenylenediamine for determination of 6-mercaptopurine

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Abstract

A MnO2 nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots (CQDs) and o-phenylenediamine (OPD) has been developed for the detection of the anticancer drug 6-mercaptopurine (6-MP). CQDs with strong fluorescence are synthesized via the one-step hydrothermal method. MnO2 nanosheets as an oxidase-mimicking nanomaterial directly oxidize OPD into 2,3-diaminophenazine (DAP) which has a fluorescence emission at 570 nm, whereas the fluorescence of CQDs at 445 nm is then reduced by the DAP through the inner filter effect (IFE) under a single excitation wavelength (370 nm). After adding 6-MP, MnO2 nanosheets can be reduced to Mn2+ and lose their oxidase-like property, blocking the IFE with the fluorescence decrease of DAP and fluorescence increase of CQDs. The novel ratiometric fluorescence probe exhibits considerable sensitivity toward 6-MP and linear response is in the 0.46–100.0 μmol L−1 concentration range with the detection limit of 0.14 μmol L−1. Furthermore, the probe shows good selectivity when exposed to a series of interfering other organic and inorganic compounds, and biomolecules and can be applied to the detection for 6-MP in human serum samples and pharmaceutical tablets. Satisfactory recoveries of 6-MP in human serum samples are in the range 96.1–110.9% with the RSD of 1.4 to 3.2%. The amount of 6-MP is successfully estimated as 49.3 mg in pharmaceutical tablet with the RSD of about  2.2%.

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Funding

This work was financially supported by the National Natural Science Foundation of China (Grant No. 21705132) and the Fundamental Research Funds for the Central Universities (no. XDJK2019TY003).

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Authors and Affiliations

  1. Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China

    Xuanxuan An, Ruchun Chen, Qizhen Chen, Qin Tan, Shuang Pan & Xiaoli Hu

  2. College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, People’s Republic of China

    Hui Liu

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  1. Xuanxuan An
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Correspondence to Xiaoli Hu.

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An, X., Chen, R., Chen, Q. et al. A MnO2 nanosheet-assisted ratiometric fluorescence probe based on carbon quantum dots and o-phenylenediamine for determination of 6-mercaptopurine. Microchim Acta 188, 156 (2021). https://doi.org/10.1007/s00604-021-04802-4

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