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CdTe quantum dots coated with a molecularly imprinted polymer for fluorometric determination of norfloxacin in seawater

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Abstract

A fluorescent nanoprobe consisting of CdTe quantum dots (QDs) and coated with molecularly imprinted layers was prepared and successfully applied to the determination of norfloxacin (NOR) in seawater and wastewater samples. The 3-mercaptopropionic acid capped QDs were prepared and then covered with a protective silica shell. A molecularly imprinted layer was finally synthesized around the silanized QDs using 3-aminopropyltriethoxysilane as functional monomer and norfloxacin as the template. Compared with the non-imprinted polymer, the fluorescence of the nanoprobe with imprinted polymer (measured at excitation/emission wavelengths of 300/596 nm) is strongly reduced in the presence of NOR, and the imprinting factor is 8.8. Under the optimal experimental conditions, the detection limit of the nanoprobe is 0.18 μM, and response is linear between 0.5 - 28 μM of NOR. The relative standard deviation of the detection of NOR is <7.2%. In order to evaluate the practicality of the probe, wastewater and seawater samples spiked with norfloxacin were analyzed by this method, and recoveries ranged from 96.2 - 106.0%.

Schematic presentation and fluorescence spectrum of fluorescent nanoprobe with selectivity for norfloxacin (NOR). CdTe quantum dots (QDs) are used as fluorescent carriers, 3-aminopropyltriethoxysilane (APTES) as template molecules, tetraethyl orthosilicate (TEOS) as crosslinking agent, and aqueous ammonia as initiator.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 41876078), the Public Science and Technology Research Funds Projects of Ocean (No. 201505034), Shandong Provincial Natural Science Foundation of China (ZR2018MD016) and the Special Fund for Basic Scientific Research in Central Colleges and Universities (No. 201713059). We especially thank the anonymous reviewers for their constructive commends.

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Authors and Affiliations

  1. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, People’s Republic of China

    Tian Shi, Hailu Fu, Liju Tan & Jiangtao Wang

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  1. Tian Shi
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  2. Hailu Fu
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Correspondence to Jiangtao Wang.

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Shi, T., Fu, H., Tan, L. et al. CdTe quantum dots coated with a molecularly imprinted polymer for fluorometric determination of norfloxacin in seawater. Microchim Acta 186, 362 (2019). https://doi.org/10.1007/s00604-019-3440-7

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