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Immortalized cell lines derived from dental/odontogenic tissue

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Abstract

Stem cells derived from dental/odontogenic tissue have the property of multiple differentiation and are prospective in tooth regenerative medicine and cellular and molecular studies. However, in the face of cellular senescence soon in vitro, the proliferation ability of the cells is limited, so studies are hindered to some extent. Fortunately, immortalization strategies are expected to solve the above issues. Cellular immortalization is that cells are immortalized by introducing oncogenes, human telomerase reverse transcriptase genes (hTERT), or miscellaneous immortalization genes to get unlimited proliferation. At present, a variety of immortalized stem cells from dental/odontogenic tissue has been successfully generated, such as dental pulp stem cells (DPSCs), periodontal ligament cells (PDLs), stem cells from human exfoliated deciduous teeth (SHEDs), dental papilla cells (DPCs), and tooth germ mesenchymal cells (TGMCs). This review summarized establishment and applications of immortalized stem cells from dental/odontogenic tissues and then discussed the advantages and challenges of immortalization.

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Funding

This study was supported by the National Natural Science Foundation of China (No. 81900996 to DZS) and the Key Research and Development Program of Sichuan Province, China (2022YFS0120).

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Authors and Affiliations

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, National Center for Stomatology, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China

    Yanglin Zeng, Liu Liu, Dingming Huang & Dongzhe Song

  2. Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China

    Dingming Huang & Dongzhe Song

Authors
  1. Yanglin Zeng
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  2. Liu Liu
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  3. Dingming Huang
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  4. Dongzhe Song
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Yanglin Zeng completed the writing and prepared the tables and figures. Liu drafted the pictures. Dingming Huang and Dongzhe Song contributed to the conception, design, and review of the manuscript.

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Correspondence to Dongzhe Song.

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Zeng, Y., Liu, L., Huang, D. et al. Immortalized cell lines derived from dental/odontogenic tissue. Cell Tissue Res 393, 1–15 (2023). https://doi.org/10.1007/s00441-023-03767-5

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