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Expression of pigment-epithelium-derived factor during kidney development and aging

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Abstract

Inhibitors and stimulators of endothelial cell growth are essential for the coordination of blood vessel formation during organ growth and development. In the adult kidney, one of the major inhibitors of angiogenesis is pigment-epithelium-derived factor (PEDF). We have analyzed the expression and distribution of PEDF during various stages of renal development and aging with particular emphasis on the formation of functional glomeruli. We show that PEDF gene expression and protein levels in the kidney significantly increase with age. We have detected PEDF in the mesenchyme and endothelial cells at all developmental stages studied, in all regions of the nephrogenic zone in which the formation of new blood vessels is associated with the development of nephrons and collecting ducts, and in mature podocytes in the adult kidney. Our results are the first to suggest that PEDF is important in early renal postnatal development, that it could be relevant to the maturation of glomerular function and the filtration barrier formed by these cells, and that it may serve as an anti-angiogenic modulator during kidney development.

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Acknowledgments

We thank Prof. Dr. Ralph Witzgall, Institute for Molecular and Cellular Anatomy, University of Regensburg, for helpful comments and technical support, and Mrs. Eva-Maria Stoerr for technical assistance.

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Authors and Affiliations

  1. Department of Neurosurgery, University Clinic of Regensburg, Franz-Josef-Strauss Allee 11, 93053, Regensburg, Germany

    Ana Luisa Pina, Marion Kubitza & Alexander Brawanski

  2. Department of Ophthalmology, Yale University School of Medicine, New Haven, Conn., USA

    Joyce Tombran-Tink

  3. Department of Neuroscience, Penn State University, Hershey, Pa., USA

    Joyce Tombran-Tink

  4. Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany

    Sabine Kloth

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  1. Ana Luisa Pina
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  2. Marion Kubitza
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  5. Sabine Kloth
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Correspondence to Ana Luisa Pina.

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Ana Luisa Pina and Marion Kubitza contributed equally to this work.

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Pina, A.L., Kubitza, M., Brawanski, A. et al. Expression of pigment-epithelium-derived factor during kidney development and aging. Cell Tissue Res 329, 329–338 (2007). https://doi.org/10.1007/s00441-007-0420-8

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