Abstract
Petal spots are widespread in plants, they are important for attracting pollinators and as economic traits in crop breeding. However, the genetic and developmental control of petal spots has seldom been investigated. To further clarify the development of petal spots formation, we performed comparative transcriptome analysis of Lilium davidii var. unicolor and Lilium davidii petals at the full-bloom stage. In comparison with the parental species L. davidii, petals of the lily variety L. davidii var. unicolor do not have the distinct anthocyanin spots. We show that among 7846 differentially expressed genes detected, LdMYB12 was identified as a candidate gene contributing to spot formation in lily petals. The expression level of LdMYB12 in the petals of L. davidii was higher than that in L. davidii var. unicolor petals. Moreover, overexpression of LdMYB12 led to the appearance of spots on the petals of L. davidii var. unicolor, accompanied by increased expression of anthocyanin synthesis-related genes. Taken together, these results indicate that abnormal expression of LdMYB12 contributes to petal spot deficiency in L. davidii var. unicolor.
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Data availability
The Illumina reads have been deposited in the Sequence Read Archive (SRA) Database at NCBI (http://www.ncbi.nlm.nih.gov/sra) and are available under study accession number PRJNA936201.
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Funding
This research was supported by the Project for Crop Germplasm Resources Conservation of Jiangsu (2021-SJ-011), the Modern Agricultural Industry Technology System in Jiangsu (JATS [2022] 007), and the High Level Talent Project of the Top Six Talents in Jiangsu (NY-077).
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Zhang, X., Xu, S., Pan, X. et al. Low LdMYB12 expression contributes to petal spot deficiency in Lilium davidii var. unicolor. Mol Genet Genomics 298, 1545–1557 (2023). https://doi.org/10.1007/s00438-023-02080-8
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DOI: https://doi.org/10.1007/s00438-023-02080-8