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Whole-exome sequencing deciphers the genetic profile of visual impairments in patients from Southwest Iran

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Abstract

Genetic ocular diseases are heterogeneous disorders. Recent advances have led to a paradigm shift in the discovery of eye disease-associated genetic variants from linkage and genome-wide association studies to next-generation sequencing-based genome studies. The aim of the current study was to investigate the spectrum of possible vision impairment-related variants in 66 Iranian patients. Whole-exome sequencing (WES) technology followed by bioinformatics analysis, Sanger validation, and co-segregation study were done to find eye disease-causing variants in the patients with vision impairments from Southwest Iran. WES revealed disease-causing variants in 82% of the enrolled cases. WES of understudied cohorts presented an effective strategy for determining pathogenic variants in heterogeneous eye diseases and demonstrated the distribution of causative genetic mutations in Iranian patients. The present data could provide the potential to accelerate genetic screening and a reference for treatment modalities for patients with different types of eye disorders from Southwest Iran.

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Acknowledgements

We acknowledge all the help from our colleagues in Narges Medical Genetics and Prenatal Diagnosis laboratory. We appreciate the cooperation of patients and their parents in the present study.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mina Zamani, Sahar Sedighzadeh, Tahereh Seifi & Hamid Galehdari

  2. Narges Medical Genetics and Prenatal Diagnosis Laboratory, Ahvaz, Iran

    Mina Zamani, Sahar Sedighzadeh, Tahereh Seifi, Samira Negahdari, Jawaher Zeighami, Alireza Sedaghat & Gholamreza Shariati

  3. Legal Medicine Research Center, Legal Medicine Organization, Ahvaz, Iran

    Samira Negahdari

  4. Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Alireza Sedaghat

  5. Department of Medical Genetics, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Gholamreza Shariati

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  1. Mina Zamani
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  2. Sahar Sedighzadeh
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  3. Tahereh Seifi
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  4. Samira Negahdari
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  5. Jawaher Zeighami
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  6. Alireza Sedaghat
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  7. Gholamreza Shariati
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  8. Hamid Galehdari
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Correspondence to Gholamreza Shariati or Hamid Galehdari.

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Communicated by Shuhua Xu.

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Zamani, M., Sedighzadeh, S., Seifi, T. et al. Whole-exome sequencing deciphers the genetic profile of visual impairments in patients from Southwest Iran. Mol Genet Genomics 297, 1289–1300 (2022). https://doi.org/10.1007/s00438-022-01917-y

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  • DOI: https://doi.org/10.1007/s00438-022-01917-y

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