Identification of a novel redox switch between metabolism and cardiac function using HyPer power

functions

the cardiovascular system [5]. Most studies addressing this question so far were using transgenic mouse models overexpressing or deleting the major endogenous source of H 2 O 2 in the cardiovascular system, the NADPH oxidase Nox4. In the vasculature, Nox4 knockouts provide anti-atherosclerotic effects and endothelial protection [4,8]. In the myocardium, the role of Nox4 is less well-defined [5]. Several studies using Nox4 knockouts showed protective effects in the heart, but upregulation of Nox4 in the myocardium can also cause cardiac remodeling via Akt-mTOR and NFκB signaling pathways [10].
The type of transgenic models is important for the analysis of ROS in the cardiovascular system [9]. Transgenic models can differ in the type of affected ROS, their intracellular or tissue-specific ROS localization, and their effect on ROS concentration. High or low ROS concentrations can mediate different, sometimes opposite effects. Low H 2 O 2 concentrations in the nanomolar range might be protective, while high H 2 O 2 concentration above 100 µm can induce cytotoxic effects and cell death. The sensitivity to ROS differs between cells and tissues due to their antioxidative capacity. Furthermore, the assays used to measure ROS needs to be critically discussed [2]. Taking these points into account, the development of appropriate transgenic models is crucial to test the role of H 2 O 2 as a potential molecular switch between metabolic and cardiovascular functions in vivo (Fig. 1).
This important scientific question has been addressed by Dörthe Katschinski and her colleagues [6]. The paper has been recently published in Nature Communications and is highlighted as the Paper of the Month by the German Physiological Society. In this excellent study, Nanadikar et al. developed a novel chemogenetic transgenic mouse model of inducible overexpression of the hydrogen peroxide sensor HyPer using D-amino acid oxidase (DAO) (HyPer-DAO mice) in cardiomyocytes. The primarily nuclear localization 1 3 of HyPer in this model resulted in a better fine tuning of intracellular H 2 O 2 formation in comparison to classical overexpression or knockout of endogenous H 2 O 2 sources like Nox4. The HyPer probe was reversibly inducible in response to the DAO-specific substrate D-Alanine (D-ala). Using this model, increased endogenous H 2 O 2 formation in cardiomyocytes was leading to impaired cardiac contractility. The authors identified novel redox-sensitive target proteins by proteomics. Hydrogen peroxide could reversibly oxidize protein cysteine thiols (-SH) to sulfenic acid (-SOH) finally resulting in disulfide-bridge formation. Such a reversible oxidation of a protein cysteine thiol by H 2 O 2 is considered as a redox switch. Most of the redox-sensitive proteins identified in this screen had cysteine residues. The authors focused on proteins involved in mitochondrial metabolism and discovered the γ-subunit of the TCA cycle enzyme isocitrate dehydrogenase (IDH) 3 as a novel redox switch linking metabolism and cardiac function. HyPer-DAO overexpressing HEK cells exhibited a reversible redox modification and activity of IDH3 after activation of DAO in vitro. Endogenously produced H 2 O 2 impaired ATP generation in the mitochondria. In a series of elegant experiments using microsecond molecular dynamics simulations and cysteine-gene-edited cells, they could prove that IDH3γ cysteine (Cys) 148 and 284 are critically involved in the H 2 O 2 -dependent regulation of IDH3 activity. Finally, they provide evidence that the redox modification of IDH3γ Cys148 and Cys284 is responsible for ATP production in the mitochondria. In summary, these data shed light into a novel mechanism how mitochondrial metabolism could be linked via redox-sensitive mechanisms to cardiac function.
What are the implications of these exciting findings for cardiovascular physiology and pathophysiology? First, redox-sensitive modifications of these and other proteins or cysteine residues could provide novel regulatory mechanisms to fine tune the cross-talk between metabolism and cardiac function. Second, this novel transgenic mouse model might be used to study the role of H 2 O 2 in important processes like myocardial ischemia and reperfusion. Especially interesting would be a detailed molecular analysis of the most probably redox-sensitive mechanisms of stunning [3].
In conclusion, the use of HyPer power might provide stunning novel views into redox-sensitive processes of cardiovascular physiology and pathophysiology. Data availability Not applicable.

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