Mechanisms linking bariatric surgery to adipose tissue, glucose metabolism, fatty liver disease and gut microbiota

Purpose In the last 20 years, bariatric surgery has achieved an important role in translational and clinical research because of obesity comorbidities. Initially, a tool to lose weight, bariatric surgery now has been shown to be involved in several metabolic pathways. Methods We conducted a narrative review discussing the underlying mechanisms that could explain the impact of bariatric surgery and the relationship between obesity and adipose tissue, T2D, gut microbiota, and NAFLD. Results Bariatric surgery has an impact in the relation between obesity and type 2 diabetes, but in addition it induces the white-to-brown adipocyte trans-differentiation, by enhancing thermogenesis. Another issue is the connection of bariatric surgery with the gut microbiota and its role in the complex mechanism underlying weight gain. Conclusion Bariatric surgery modifies gut microbiota, and these modifications influence lipid metabolism, leading to improvement of non-alcoholic fatty liver disease.


Introduction
Obesity is a major public health problem that has been increasing worldwide [1].This multifactorial disease is associated with an increased risk of developing several medical conditions, such as insulin resistance, hypertension, dyslipidaemia, non-alcoholic fatty liver disease (NAFLD), cardiovascular disease and even some types of cancers.In addition, it is the major risk factor for type 2 diabetes (T2D) [2,3].
Bariatric surgery has been demonstrated to successfully achieve significant and sustainable weight loss and improvement of associated comorbidities [4,5].The benefits observed in metabolic disease, independently of weight loss, define bariatric surgery as metabolic surgery.In 1978, L. Varco described metabolic surgery as "the operative manipulation of a normal organ system to achieve a biological result for a potential health gain" [6].The first surgical management for obesity was in 1952, when V. Henrikson, a Swedish surgeon, performed a 105-cm small bowel resection on a woman with obesity [7].Since then, there have been six historically dominant procedures in bariatric surgery [6]: jejunoileal by-pass (JIB), Roux-en-Y gastric by-pass (RYGB), then modified in one anastomosis gastric by-pass (OAGB), vertical banded gastroplasty (VBG), biliopancreatic diversion (BPD) and its modification duodenal switch (DS), adjustable gastric banding (AGB) and sleeve gastrectomy (SG) (Table 1).Globally, the number of surgical procedures has dramatically increased from 146,301 procedures carried out in 2003 to 604,223 surgical procedures in 2018 [8,9].SG is the most performed bariatric procedure (55.4%), followed by RYGB (29.3%), then OAGB (6.6%), whereas no other single surgical procedure exceeds 1.5% [8].
These procedures were originally designed to achieve weight loss, but now it is known that bariatric surgery involves molecular, anatomical and physiological alterations even by weight-independent mechanisms, with long-term effects [4,5].this, intestinal by-pass is seldom performed in superobese patients; thus, Scopinaro proposed an intestinal by-pass procedure called BPD [12].BPD includes a partial gastrectomy with closure of the duodenum and a long intestinal by-pass with a Roux limb of 250-cm length and a 50-cm common channel.The procedure was then modified in DS where the distal gastrectomy was a sleeve gastrectomy, and the common channel had a length of 100 cm [13].However, surgical complexities and the risk of long-term complications have limited the popularity of these procedures.
Restrictive procedures reduce the stomach capacity by creating a smaller chamber for food intake.One of the earliest restrictive procedures was VBG, which involved the creation of a vertical pouch of about 50-mL volume with an outlet flow encircled by a fixed band to prevent it from dilatation [14].The concept of an external gastric band sustains the use of AGB.Initially not adjustable, and then adjustable thanks to a subcutaneous port, AGB became popular by laparoscopic approach [15].However, an overall modest performance and band complications have reduced the number of this procedure over the years.The most recent restrictive procedure is the SG.This procedure was initially performed as the first step of a two-staged DS in high-risk patients who undergo SG and after about 1 year, intestinal by-pass [16].However, many patients obtained good results with the SG alone; thus, it was adopted as a stand-alone procedure.In SG, the gastric greater curvature is resected, and thus, the stomach is reduced to a narrow tube.The removal of the greater curvature induces weight loss but also hormonal changes, such as a reduction of serum ghrelin levels, which help promote early satiety and prolonged satiation.
RYGB is the most popular version of gastric by-pass, originally proposed by Mason [17].The procedure, currently considered the "gold standard" in bariatric surgery, includes a vertical lesser curvature pouch, coupled with a jejuno-jejunostomy, in addition to a gastro-jejunostomy and a common limb of around 150 cm.Thus, it is a combination of restrictive and malabsorptive procedures.Of course, the complete procedure of DS, which includes SG and intestinal by-pass, can also be considered a combined restrictive and malabsorptive procedure.Another combined procedure is OAGB, a gastric by-pass that involves only one anastomosis-an end-to-side anastomosis between the gastric pouch and a jejunum loop 150-250 cm from the Treitz ligament [18].Patients undergoing OAGB were found to have more nutritional deficiencies compared with those who underwent RYGB [19].To avoid these problems, some surgeons suggest reducing the length of the biliopancreatic limb to less than 150 cm [20].
In recent years, many endoscopic techniques have been proposed to induce weight loss, especially in superobese highrisk patients [21].The most popular endoscopic technique is the intragastric balloon insertion that decreases the intraluminal gastric volume to induce early satiety during food intake [8].The device is temporary and must be removed within 6 months, mostly because weight loss is transient.The intragastric balloon, in fact, is used as a bridge to definitive bariatric surgery.Other endoscopic procedures include endoscopic sleeve gastroplasty involving full-thickness sutures and primary obesity surgery endoluminal (POSE) procedure that creates up to ten gastric plications.Both procedures reduce the gastric cavity by remodelling the stomach [21].

Bariatric surgery and adipose tissue
Adipose tissue is recognized as an endocrine organ implicated in the physiopathology of obesity and its comorbidities [22,23].As the organ is a self-contained group of tissues that perform a specific function, in the adipose organ, we can distinguish two different adipose tissues, the white adipose tissue (WAT) storing energy and the brown adipose tissue (BAT) using energy for thermogenesis [24].The WAT can be divided into two broad categories, visceral adipose tissue (VAT) located in the peritoneal cavity and subcutaneous adipose tissue (SAT) located under the skin.The WAT-BAT cooperation consists of the reciprocal ability of conversion in relation to physiologic requirement of the body [25].
The endocrine function of the adipose tissue is carried out by the secretion of hundreds of different signalling proteins called adipokines into the circulation [26].These include leptine that suppresses appetite when lipid storage is high and stimulates pro-inflammatory immune response [27] and adiponectine that acts on other organs such as the liver and muscle, and is highly correlated with metabolic derangements of obesity and T2D [28].
Although the adipose organ of animals and humans with obesity is increased at both subcutaneous and visceral sites, VAT alone is responsible for the onset of obesity-associated metabolic disorders [29][30][31].These disorders result from adipose tissue dysfunction and inflammation.In obese mice and humans, inflammatory cells infiltrate adipose tissue producing inflammatory mediators that may explain the correlation between visceral fat and cardiovascular and metabolic complications, such as insulin resistance and T2D [3,[32][33][34].Macrophages are the main inflammatory cells found in inflamed adipose tissue.Hypertrophic adipocytes die and remnants of dead adipocytes are surrounded by active MAC2 immunoreactive macrophages reabsorbing the large debris.These form a characteristic histopathology feature denominated crown-like structure (CLS) [33].Furthermore, VAT is composed by more fragile adipocytes compared to those of SAT; in obese subjects, these adipocytes die with a smaller critical death size inducing major inflammation [34].
Why visceral fat behaves differently from SAT in animals and humans with obesity is a concept that is important to understand.The size of adipocytes in VAT is smaller than that of subcutaneous fat.The reason for this difference is not known, but a hypothesis can be proposed.Since a large proportion of VAT in young people is composed by BAT and age is an important factor inducing BAT to WAT conversion, a daring hypothesis for the different size could be that subcutaneous fat originates from WAT adipocyte precursors, while visceral fat originates from conversion of BAT.This theory was recently demonstrated using a mouse-model lacking ATGL (adipose-triglycerides lipase) [3].In these mice, adipocytes cannot use stored lipids for thermogenesis and BAT is converted into a WAT-like tissue.This WAT-like tissue derived from BAT conversion is more prone to death as shown by the number of CLS in the WAT-like tissue compared with regular WAT with the same size of adipocytes.WAT, producing adipokines, growth factors, enzymes and active immune cells such as macrophages and T cells, takes part in the progression of inflammation in obesity [35].These data offer an explanation to the higher level of inflammation in VAT and as the visceral obesity is the clinical condition more frequently associated to T2D in obese patients [3].
Studies of depot-specific fat mass show how bariatric surgery induces both VAT and SAT reduction with a metabolically beneficial redistribution among different anatomic depots [36][37][38][39].Through reduction of WAT, bariatric surgery reverses the balance between pro-inflammatory and anti-inflammatory mediators [40,41].Recent studies show that post-surgical circulating levels of adiponectin and leptin are significantly increased and decreased respectively [41,42].Similarly, serum inflammatory mediators IL-6 and TNF are downregulated [36,43].Adiponectin reduces fat storage and inflammation, increases fibrinolysis and additionally activates 5′-AMP-activated protein kinase (AMPK) after surgery.Indeed, subcutaneous adipose tissue levels of AMPK increase after metabolic surgery [44], and AMPK has been associated with improvements in inflammation, oxidative stress, mitochondrial biogenesis and insulin resistance in several tissues [45].However, it is unclear whether AMPK reduces oxidative stress or whether the reduction of oxidative stress suppresses AMPK [44].
Preclinical and clinical studies suggest that bariatric surgery induces changes in BAT by enhancing thermogenesis [46].Increasing BAT size has been observed after RYGB [47,48].This effect could be due to an increase of glucagonlike peptide 1 (GLP-1) in RYGP, which improves thermogenesis and increases BAT size [49,50].On the other hand, no significant changes in BAT size were observed in patients submitted to SG [51].However, SG may enhance BAT thermogenesis contributing to improve glycaemic control [47,52].How BAT activation after surgery modulates the energy balance and remission of T2D is unknown.One possible mechanism may act through bile acids that promote BAT thermogenesis via interaction with the thyroid system and GLP-1 receptor signalling [53,54].

Bariatric surgery and T2D
T2D is the most frequent form of diabetes accounting for about 90% of all diagnosis of diabetes.It represents one of the most important pathologies in Western countries, with 592 million T2D patients expected by 2035 [55,56].T2D is the fifth leading cause of death [57].Thus, all efforts to prevent or treat this disease must be encouraged, and all aspects of related scientific research on causes and physiopathology should be strongly sustained by governments worldwide.
T2D is associated with progressive loss of pancreatic beta-cell function and mass [58].As previously reported, obese fat in mice and humans is infiltrated by macrophages [32,59].Macrophages cause a chronic low-grade inflammation producing mainly TNFa and IL6.The mechanism by which tissue inflammation influences insulin sensitivity is unclear, but these molecules have been proven to interfere with the insulin receptor causing insulin resistance [60][61][62][63].The gradual transition of insulin resistance to T2D is linked with a change in pancreatic islet composition.During compensated insulin resistance, pancreatic islets are conspicuous by their hyperplasia, resulting from increased cell number and size [58].But as compensation fails, islet mass gradually decreases, and beta-cells become depleted of their characteristic insulin secretory granules, ending with a functional exhaustion which coincides with the onset of T2D [58,63,64].Recently, it has been demonstrated that obese mice have an increased noradrenergic innervation of Langerhans islets, with data supporting nerve-epithelial contacts with beta-cells [58,65].A recent paper confirmed these data in humans [66].Thus, considering the well-known inhibitory activity of noradrenaline on insulin secretion, the hypothesis is that the lack of insulin secretion inducing T2D after a period of hyper-production is not due to beta-cell exhaustion, but to beta-cell inhibition by increased noradrenergic innervation.For unknown reasons, bariatric surgery could induce a de-innervation process in the Langerhans islets restoring the insulin secretory activity of beta-cells.Data supporting a direct innervation of pancreatic islets by neurons located in the intestinal wall are in line with the idea that surgical removal or intestinal by-pass could influence pancreatic islet innervation [67].
In the last decade, a considerable amount of high-quality evidence supported that bariatric surgery has an effective role in the treatment of T2D, with an improvement of glucometabolic profiles and a complete remission of diabetes [68].The effects of metabolic surgery are stable over time, with a substantially greater effect at five years compared with medical treatment [69].Some factors contribute to the post-operative diabetes response after surgery.Patients with a BMI > 30 kg/m 2 and those with a BMI < 30 kg/m 2 have distinct remission predicting factors.Low HbA1c is a predictor of remission in low-high-BMI patients while the length of time in which the patients are affected by diabetes is a predictor in high-low-BMI patients [70].
In fact, in animal models of obesity and in humans undergoing different types of bariatric surgery, T2D improves within days to weeks, whereas weight loss occurs much more slowly [68].The weight-independent mechanisms involved are not completely understood, but they include the incretin effect of GLP1, alterations in bile acids and changes in gut microbiota composition [5].GLP1 increases dramatically after bariatric surgery, independently of both calorie reduction and weight loss [71].Therefore, reduction of food intake (anorexic effect of surgery), reduction of insulin resistance (long-term weight reduction after surgery) and increase of insulin secretion (which is weight loss independent) improves diabetes.Bile acids probably interact with the gut microbiota in the duodenum and proximal jejunum.The gut microbiota has an interdependent relationship with bile acids, whereby bile acids affect the microbiota composition by altering bacterial membrane integrity, and the gut microbiota can alter bile acid synthesis and function, including bile acid deconjugation, dihydroxylation, oxidation and epimerization [72,73].The effects and interactions of these systems are illustrated in Fig. 1.

Bariatric surgery and gut microbiota
The human gut, mainly the colon, holds the greatest numbers of microbiota in the organism.Humans and microorganisms have long benefited from this symbiotic relationship, yet our understanding of the extent and meaning of this co-existence has been limited due to the lack of reliable and effective tools to study it.Recent evidence has suggested a role for alterations in the gut microbiota in promoting or aggravating different diseases such as obesity [74].
Components of gut microbiota are now considered to play a significant role in several fields, such as the regulation of intestinal function, metabolism, behaviour and immunity [75].
The adult gut microbiota is dominated by two phyla, Firmicutes and Bacteroidetes, which constitute about 90% of all the bacterial species in the gut [74].Many studies have shown a relative decrease of Bacteroidetes with a relative increase in Firmicutes in the obese microbiota, but the findings are still weak [76][77][78][79].A difficult question is whether changes in the intestinal microbiota precede the development of obesity or reflect the obese phenotype.Of course, diet, 101 Page 6 of 13 microbiota and immunity participate in the development of obesity [80][81][82][83].
Several theories discuss the hypothesis that gut microbiota can induce obesity.One hypothesis is that the gut microbiota of individuals with obesity is capable of fermenting dietary carbohydrates that increases the rate of short-chain fatty acids (SCFAs), providing extra energy, which are then stored as lipids or glucose [77,78,84].In fact, Firmicutes, which are major producers of the SCFAs, are increased in the obese faecal microbiota [76].This theory is supported by transplanting obese faecal microbiota in germ-free mice and finding a higher level of Firmicutes in faecal samples and an increase in body fat [76].Carbohydrate and lipid metabolism are highly influenced by microbiota that increase the bioavailability of monosaccharides, and the subsequent induction of de novo hepatic lipogenesis.The microbiota of genetically obese mice is rich in enzymes involved in the fermentation of dietary fibre; the products of dietary fibre fermentation include SCFAs such as acetate, propionate and butyrate [74], which generally improve glucose and energy homeostasis [85].Preclinical and human studies show that obese individuals present higher faecal concentrations of SCFAs than lean controls [75,86].However, the role of SCFAs is controversial because obesity-inhibiting properties have been described [87].SCFAs suppress the inflammatory immune response in the gut [88,89] and they are involved in the release of GLP-1 and leptine, which may downregulate appetite and thus reduce caloric intake [90].
Weight loss interventions, as bariatric surgery, induce a decrease in faecal SCFAs, mainly due to low-carbohydrate diets [87].This reduction could be an indication of reduced efficiency with which energy is harvested from dietary SCFAs during weight loss among overweight or obese individuals [75,87].Given the benefits of SCFA on colon cancer risk [91], studies are needed to clarify if the decrease of SCFA is a potential adverse effect of weight loss.
Another theory is that a key process in the biological physiology of obesity includes systemic inflammatory changes.
The systemic increased levels of adiponectin and inflammatory cytokines, such as tumour necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), has also been associated with hypertrophy of adipose tissue and with augmented risk of metabolic disorders such as cardiovascular diseases, fatty liver disease and T2D [74].Despite these various association studies, the causal pathways between obesity, inflammation and metabolic disease remain incompletely understood.The presence of low-grade systemic inflammation associated with obesity usually involves a complex network of signals interconnecting several organs [92,93].Understanding the mechanisms that regulate gut microbiota homeostasis and dysbiosis will lead to a better comprehension of the inflammation-related pathophysiology of obesity and consequently could provide an avenue for interventions aimed at modulating gut microbiota in individuals with obesity [94,95].
The ingestion of high fat diets could be a facilitating factor in the disruptions of gut microbiota homeostasis in obesity [74,95].These diet-induced changes in the microbiota physiology can cause low-grade systemic inflammation in obesity and may even precede or predispose to obesity [94][95][96][97][98]. Changes in the composition of gut microbiota increase intestinal mucosal inflammation with changes in gut permeability.Together, these processes can result in increased metabolic endotoxemia and in an increase of components such as plasma lipopolysaccharides (LPS) within the circulating system [99].The gut microbiota-related inflammatory changes have been linked to activation of tolllike receptor 4 (TLR4) signalling and to a resulting increase in intestinal levels of LPS [100].Studies have also shown that increased levels of LPS, together with TLR4, are risk Fig. 1 The cross-linking between bariatric surgery and adipose tissue, glucose metabolism, gut microbiota and NAFLD.Restrictive and/or malabsorptive surgeries such as SG and RYGB have important effects on lipid and glucose metabolism by direct action or through modification of gut microbiota.SCFAs, short-chain fatty acids; GLP1, glucagon-like peptide 1 Page 7 of 13 101 factors for obesity, insulin resistance and cardiovascular diseases [101][102][103].
Significant changes in gut microbiota have been noted after bariatric surgery, specifically with increases in Bacteroidetes, Fusobacteria, Verrucomicrobia and Proteobacteria and a reduction of Firmicutes, Clostridiales, Clostridiaceae, Blautia and Dorea [55,104,105].An increase in Bacteroidetes species has been correlated with a reduction in body fat mass and leptin, while the Firmicutes responsible for dietary carbohydrate fermentation and energy harvesting are decreased [106,107].
Most of the changes in microbial composition occurred within 3 months and those changes were maintained up to a year [108].This points out how remodelling of the microbial community occurred mainly within the first 3 months after surgery [108].
Gut microbiota modifications are different between bariatric procedures [109].Bacteroides vulgatus, a bacteria increased in patients with obesity and positively correlated with glycaemic status, is reduced significantly after SG, whereas it is not significantly affected by either post-AGB or post-RYGB [110].Furthermore, SG also increases Faecalibacterium prausnitzii, another bacterium decreased in obese subjects with T2D that increases post-RYGB [111].These data allow hypothesizing that the change in these bacteria could be involved in the glucose improvement observed after SG; however, this is still unclear.In another study, comparing SG and RYGB in a small sample size, Murphy et al. observed that although SG was associated with functional changes in gut microbiota, these were fewer than those observed post-RYGB43.Furthermore, in another study comparing SG and RYGB, both procedures induced similar clinical improvement, but gut microbiota modifications involved distinct pathways according to the surgical technique [112].
Post-RYGB patient gut microbiota reduced body weight gain when transferred into germ-free mice.These effects appear to be part of the weight loss-independent mechanisms of RYGB, as the germ-free mice colonized with the microbiota from post-RYGB patients gained 43% less body fat than mice colonized with the microbiota from weightmatched patients who did not have surgery [113].
RYGB produces significant metabolic changes, including decrease in plasma bile acid content and increases in various amines production, which reflect changes in the microbial metabolism of precursors like choline [114].
Bariatric surgery affects bile acids (BAs) metabolism [110].BAs influence glucose metabolism by increasing insulin sensitivity and reducing gluconeogenesis [115] through increased secretion of GLP-1 and activation of TGR5, improving the energy balance [116].Moreover, BAs play a role in the gut microbiota composition and in the weight loss after bariatric surgery [117].A recent study conducted by Ilhan et al. focused attention on the gut microbiota of obese patients who had underwent RYGB.Surgery causes a reduction in faecal BA concentration, which relates to changes in microbiota composition, and the gut microbiota itself is involved in the modulation of BAs metabolism [118].In fact, the anatomical changes made in RYGB increase the amount of BAs reaching the lower intestine, thus allowing conjugated BAs to be actively reabsorbed in the terminal ileum and primary BAs to enter the colon and be transformed into secondary BAs by the gut microbiota [111].These changes are linked with fatty liver disease.In NAFLD patients, the serum primary/secondary BAs ratio is significantly higher compared to controls and correlates with the severity of NAFLD [119].Bariatric surgery produces a significant repopulation of the gut microbiota and a reversal of the circulating primary/secondary BAs ratio, thus inducing metabolic improvements with positive effects on NAFLD and metabolic syndrome [119].

Bariatric surgery and NAFLD
NAFLD is defined as more than 5% fat accumulation in hepatocytes [120].In adult population, NAFLD has a high prevalence, especially in people with obesity (65.7%) and T2D (74%) [121][122][123].The association between NAFLD and T2D can be explained by insulin resistance, dyslipidaemia and the accumulation of liver triglycerides in NAFLD and beta-cell defect in T2D [124].NAFLD can progress from simple hepatic steatosis to steatohepatitis (NASH), which is characterized by inflammation and hepatocyte degeneration.A small proportion of NASH will further progress to liver fibrosis/cirrhosis and hepatocellular carcinoma.Obesity is a dominant risk factor for development of NAFLD and NASH, with 4.5-fold increased risk of hepatocellular carcinoma [125].
Secretion of adropin, an insulin sensitizing factor, and of sex hormone-binding globulin (SHBG) can be observed in the liver affected by NAFLD [41].Lower levels of SHBG could be involved in the development of NAFLD and T2D, but data are still unclear [41].
Many studies have shown that dysregulation of the gut microbiota can be involved in the pathogenesis of NAFLD [126][127][128].Changes in the abundance and diversity of the gut microbiota have been linked to the progression of NAFLD; each stage of NAFLD has a special gut microbiota signature [129].
In NAFLD, Bacteroidetes are reported to be decreased, while levels of Firmicutes and Proteobacteria are increased, especially in patients with obesity [126,129,130].Changes in microbiota in NASH are reported to overlap with steatosis, with differences identified especially in patients diagnosed with NASH with fibrosis.For example, Eubacterium rectale 101 Page 8 of 13 is increased in moderately severe NAFLD, but decreased in NASH with fibrosis [129].The higher the degree of fibrosis, the higher the abundance of Proteobacteria, and this suggests the role of these bacteria in the process of liver fibrosis, although the exact mechanism is still unknown [131].
The expression of genes involved in LPS synthesis in gut microbiota is increased in NASH compared with steatosis, while increased flagellar biosynthesis gene expression in NASH indicates fibrosis.Furthermore, bacterial translocation due to increased gut permeability and increased blood levels of LPS have been associated with NAFLD [132,133].
The intestinal microbiota has the ability which seems to play a role in the pathogenesis of NAFLD, through different pathways of bacterial metabolites such as bile acids, SCFAs, amino acids, choline and ethanol [129].
Weight loss is currently the mainstay of NAFLD treatment.A 3 to 5% weight loss has been shown to reduce steatosis, and a greater weight loss of up to 10% might be necessary to improve hepatic necro-inflammation [134].However, most NAFLD patients are not able to achieve such weight loss by diet restriction, but bariatric surgery can produce up to 85% resolution of NAFLD and NASH, with an improvement of both histological and biochemical markers [135].Oxidative stress and lipid peroxidation in patients with NAFLD also improve after metabolic surgery, reducing DNA damage and the inflammatory cascade from hepatocellular injury to fibrosis and cirrhosis [135].
Analysing the effect of specific bariatric procedures, SG determines improvements in aspartate aminotransferase, alanine aminotransferase, triglycerides and high-density lipoprotein serum levels and the NAFLD resolution assessed with ultrasound imaging and histological amelioration [136][137][138][139]. RYGB leads to reduction of steatosis, lobular inflammation, ballooning degeneration and centrilobular/perisinusoidal fibrosis [140][141][142][143]. Several studies suggest that RYGB is more effective compared with SG and LAGB in terms of benefits on NAFLD, NASH and fibrosis, whereas, in other studies, SG shows a better improvement than RYGB in serum levels of hepatic enzymes [136,144,145].
As mentioned above, bariatric surgery has additional effects beyond weight loss, which contribute to amelioration of NAFLD.Post-surgical changes in the gut microbiota and bile acid circulation, as well as a decrease in portal influx of free fatty acids, may also be beneficial for metabolic syndrome and NAFLD, as suggested by recent studies [115,[146][147][148].

Future perspective
A substantial number of metabolic modifications occurs after bariatric surgery.The mechanism involved might be either weight dependent or weight independent.The crosslinking of these mechanisms is key in the long-term effects after surgery.Inflammation in visceral fat is strictly connected with insulin resistance and T2D, but it is also related with gut microbiota.However, human studies on changes in the gut microbiota are still relatively unpowered, are not always conclusive and vary across different populations.For these reasons, further research is needed to investigate how microbiota modifications are related to glucose and lipid metabolism after bariatric surgery.