Abstract.
Genomic in situ hybridization (GISH) was used to study somatic chromosomes of parental and progeny plants (all 2n=2x=14) of the bigeneric hybrid between Gasteria lutzii and Aloe aristata (Aloaceae), which is partially fertile, a rare occurrence in plants. GISH successfully distinguished between the two parental genomes in the F1 hybrid and revealed numerous genomic recombinations in chromosomes transmitted by the F1 to the back-cross progeny. The results indicate high levels of meiotic compatibility between the parental genomes, even though they differ in size by 20%. Recombination occurred at a frequency that was higher than that expected from the analysis of orcein-stained meiosis in the F1. The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.
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Received: 9 September 1996; in revised form: 21 October 1996 / Accepted: 21 October 1996
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Takahashi, C., Leitch, I., Ryan, A. et al. The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny. Chromosoma 105, 342–348 (1997). https://doi.org/10.1007/s004120050193
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DOI: https://doi.org/10.1007/s004120050193