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Enhanced expression of αVβ3 integrin in villus and extravillous trophoblasts of placenta accreta

  • Maternal-Fetal Medicine
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Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Background

Placenta accreta is one of the most serious complications in obstetrics and gynecology. Villous trophoblasts (VT) and extravillous trophoblasts (EVT) play a central role in normal placentation. Placenta accreta is characterized by abnormal invasion of EVT cells through the uterine layers, due to changes in several parameters, including adhesion proteins. Although αvβ3 integrin is a central adhesion molecule, participating in multiple invasive pathological conditions including cancer, data on placenta accreta are lacking.

Objective

To study the expression pattern of αvβ3 integrin in placenta accreta in comparison with normal placentas.

Study design

We collected tissue samples from placentas defined as percreta, the most severe presentation of placenta accreta and from normal control placentas (n = 10 each). The samples underwent protein extractions for analyses of αvβ3 expression by Western blots (WB) and a parallel tissue assessment by immunohistochemistry (IHC).

Results

WB results indicated significantly elevated αvβ3 integrin expression in the percreta samples compared to normal placentas. These elevated levels were mainly contributed by EVT cells, as demonstrated by IHC. αvβ3 integrin demonstrated a classical membranal expression in the VT cells, whereas a uniformly distributed expression was documented in the EVT cells. These patterns of the αvβ3 integrin localization were similar in both accreta and normal placental samples.

Conclusions

Enhanced αvβ3 integrin expression, mainly in extra villous trophoblasts of placenta percreta, implies for a role of this adhesion molecule in pathological placentation.

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Funding

We declare no funding support.

Author information

Authors and Affiliations

  1. Translational Hemato-Oncology Laboratory, Hematology Institute and Blood Bank, Meir Medical Center, Kfar-Saba, Israel

    Omer Weitzner, Chen Seraya-Bareket, Martin Ellis & Osnat Ashur-Fabian

  2. Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Chen Seraya-Bareket & Osnat Ashur-Fabian

  3. Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Omer Weitzner, Chen Seraya-Bareket, Tal Biron-Shental, Ami Fishamn, Yael Yagur, Keren Tzadikevitch-Geffen, Sivan Farladansky-Gershnabel, Debora Kidron, Martin Ellis & Osnat Ashur-Fabian

  4. Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel

    Omer Weitzner, Tal Biron-Shental, Yael Yagur, Keren Tzadikevitch-Geffen & Sivan Farladansky-Gershnabel

  5. Gynecological Oncology Unit, Meir Medical Center, Kfar Saba, Israel

    Ami Fishamn

  6. Department of Pathology, Sackler Faculty of Medicine, Meir Hospital, Kfar Saba, Israel

    Debora Kidron

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  1. Omer Weitzner
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Contributions

OW declare that he participated in collection of the data for this project, cleaned, assimilated and analyzed the data and wrote the manuscript. DK declare that she participated in collecting and analysis of the data. OW, TBS and OFA declare that they cleaned, assimilated and analyzed the data. OAF conceptualized the project and led data collection and analysis. CSB, TBS, AF, YY, KTG, SF, DK and ME declares that they reviewed the analysis and the manuscript and participated in editing. All authors approved this final version of the manuscript.

Corresponding author

Correspondence to Omer Weitzner.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethics approval

This study was approved by Meir medical center ethics committee.

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Informed consent was obtained from all individual participants included in the study. The authors affirm that human research participants provided informed consent for publication.

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Weitzner, O., Seraya-Bareket, C., Biron-Shental, T. et al. Enhanced expression of αVβ3 integrin in villus and extravillous trophoblasts of placenta accreta. Arch Gynecol Obstet 303, 1175–1183 (2021). https://doi.org/10.1007/s00404-020-05844-4

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