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The combination of resveratrol and conjugated linoleic acid attenuates the individual effects of these molecules on triacylglycerol metabolism in adipose tissue

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Abstract

Purpose

The combination of resveratrol + conjugated linoleic acid (RSV + CLA) did not show the body fat-lowering effect exhibited by these molecules when administered separately. This study aimed to find metabolic explanations for this situation in an experimental model of diet-induced obesity.

Methods

Thirty-six male Wistar rats were divided into four groups: rats treated with saline (control), resveratrol (RSV), conjugated linoleic acid (CLA) and a combination of these molecules (RSV + CLA).

Results

Rats treated with RSV + CLA did not show the reduction in heparin-releasable lipoprotein lipase (HR-LPL) and fatty acid synthase activities observed in RSV group or the increased HSL expression found in RSV and CLA groups. These animals showed reduced sirtuin 1 expression and CLA isomer amounts in adipose tissue. Finally, intracellular Ca2+ concentration was increased.

Conclusion

The attenuation of the effects induced in adipose tissue triacylglycerol metabolism by RSV and CLA separately, such as the decrease in lipogenesis and fatty acid uptake and the increase in lipolysis, contributes to explain the lack of body fat-lowering effect of the combination RSV + CLA.

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Acknowledgments

This study was supported by grants from the Ministerio de Ciencia e Innovación (AGL2008-01005-ALI), Instituto de Salud Carlos III (CIBERobn and RETIC PREDIMED), Government of the Basque Country (GIC 07/120-IT-265-07), University of the Basque Country (UPV/EHU) (UFI 11/32) and INGENIO-CONSOLIDER Program, Fun-C-Food CSD2007-063. RSV and CLA were generous gifts from Monteloeder (Elche, Spain) and Lipid Nutrition (Wormerveer, The Netherlands), respectively. N. Arias is a recipient of a doctoral fellowship from the Basque Country Government.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Authors and Affiliations

  1. Nutrition and Obesity Group, Department of Nutrition and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad, 7, 01006, Vitoria-Gasteiz, Spain

    N. Arias, J. Miranda, M. T. Macarulla, L. Aguirre, A. Fernández-Quintela & M. P. Portillo

  2. CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), Madrid, Spain

    N. Arias, J. Miranda, M. T. Macarulla, L. Aguirre, A. Fernández-Quintela & M. P. Portillo

  3. Department of Nutrition and Food Science, XaRTA, INSA, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain

    C. Andres-Lacueva & M. Urpi-Sarda

  4. INGENIO-CONSOLIDER Program, Fun-c-food (CSD2007-063), Ministry of Science and Innovation, Barcelona, Spain

    C. Andres-Lacueva & M. Urpi-Sarda

Authors
  1. N. Arias
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  2. J. Miranda
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  8. M. P. Portillo
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Correspondence to M. P. Portillo.

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Arias, N., Miranda, J., Macarulla, M.T. et al. The combination of resveratrol and conjugated linoleic acid attenuates the individual effects of these molecules on triacylglycerol metabolism in adipose tissue. Eur J Nutr 53, 575–582 (2014). https://doi.org/10.1007/s00394-013-0566-y

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