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Protective effects of olive oil phenolics and gallic acid on hydrogen peroxide-induced apoptosis

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Abstract

Purpose

Olive oil contains several phenolic compounds possessing antioxidant activity. The aim of this study was to investigate the protective effects of olive oil phenolic extract (OOPE) and one of its constituents, gallic acid (GA) against H2O2-induced oxidative stress and apoptotic cell death in HeLa cells, a model for human epithelial cells.

Methods

The cells were pretreated with nontoxic doses of OOPE or GA for 4, 24 and 48 h, and the intracellular reactive oxygen species (ROS) level was determined, before and after oxidative stress induction with H2O2. As an indicator of apoptosis, caspase 9 activity was measured.

Results

All pretreatments reduced ROS generation. Four hour incubation with OOPE or GA completely inhibited ROS generation. Increases in caspase 9 activity by OOPE and GA pretreatment under harsh stress conditions were inhibited 92 and 67.8%, respectively.

Conclusions

These results suggest that OOPE and GA act as powerful antioxidants against oxidative stress and exert anti-apoptotic effects.

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Acknowledgments

This study was supported by the Research Fund of Çanakkale Onsekiz Mart University (Project no: 2007/69) and the Research Fund of Istanbul University (Project no. 4120).

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Çanakkale Onsekiz Mart University, Terzioglu Campus, 17020, Çanakkale, Turkey

    Özlem Erol-Dayi

  2. Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul, Turkey

    Nazlı Arda

  3. Department of Environmental Health and Biosafety, School of Health Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Günhan Erdem

Authors
  1. Özlem Erol-Dayi
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  2. Nazlı Arda
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  3. Günhan Erdem
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Correspondence to Özlem Erol-Dayi.

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Erol-Dayi, Ö., Arda, N. & Erdem, G. Protective effects of olive oil phenolics and gallic acid on hydrogen peroxide-induced apoptosis. Eur J Nutr 51, 955–960 (2012). https://doi.org/10.1007/s00394-011-0273-5

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  • DOI: https://doi.org/10.1007/s00394-011-0273-5

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