Abstract
Narcissus tazetta is an appreciated ornamental plant worldwide with a unique sensory value. Therefore, every new insight into the behavioral aspects is crucial for the improvement of its cultivation process. The present study aimed to evaluate how the application of sodium nitroprusside (SNP), as an exogenous nitric oxide (NO) donor, can favor the flowering process in N. tazetta. Indeed, the application of different SNP concentrations (25, 50, and 100 mM) significantly increased the flowering percent and size of flowers, which was accompanied with improvement of leaf and flower scape anatomical structure. Based on the observed delay in the flowering time of Narcissus only at 100 mM SNP, the inductive late-flowering effect of NO might be dose-dependent. Furthermore, SNP triggered the accumulation of photosynthetic pigments, which was coexisted with high production of sugars. Supplying SNP also increased anthocyanins and phenolic compounds accompanied with advanced total antioxidant property in the leaves and flowers. The obtained data of H2O2, superoxide dismutase, proline, and malondialdehyde suggested no stress-inductive effect of NO in the Narcissus flowering process. Besides, the observed increase in the nitrate reductase activity in the leaves suggesting that NO can regulate diverse mechanisms in the flowering process. Overall, the results revealed the positive role of SNP in the flowering process in N. tazetta.
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References
Abd El, Gayed M (2020) Effect of bulb cutting, storage methods and hot water treatment on the propagation of Narcissus tazetta plants. J Product Dev 25(2):247–261
Abedi S, Iranbakhsh A, Ardebili ZO, Ebadi M (2021) Nitric oxide and selenium nanoparticles confer changes in growth, metabolism, antioxidant machinery, gene expression, and flowering in chicory (Cichorium intybus L.): potential benefits and risk assessment. Environ Sci Pollut Res 28(3):3136–3148
Ahmad P, Alam P, Balawi TH, Altalayan FH, Ahanger MA, Ashraf M (2020) Sodium nitroprusside (SNP) improves tolerance to arsenic (As) toxicity in Vicia faba through the modifications of biochemical attributes, antioxidants, ascorbate-glutathione cycle and glyoxalase cycle. Chemosphere 244:125480
Ahmad P, Alyemeni MN, Wijaya L, Ahanger MA, Ashraf M, Alam P, Paray BA, Rinklebe J (2021) Nitric oxide donor, sodium nitroprusside, mitigates mercury toxicity in different cultivars of soybean. J Hazard Mater 408:124852
Akram MN, Verpoorte R, Pomahačová B (2021) Effect of bulb age on alkaloid contents of narcissus pseudonarcissus bulbs. South Afr J Bot 136:182–189
Arasimowicz M, Floryszak-Wieczorek J (2007) Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Sci 172(5):876–887
Baker DE, Amacher MC (1983) Nickel, copper, zinc, and cadmium. Methods of soil analysis: part 2. Chem Microbiol Prop 9:323–336
Barrett SC, Lloyd DG, Arroyo J (1996) Stylar polymorphisms and the evolution of heterostyly in Narcissus (Amaryllidaceae). In: Floral Bio. Springer, pp 339–376.
Bates L, Waldren R, Teare I (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44(1):276–287
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Bremner J (1960) Determination of nitrogen in soil by the Kjeldahl method. J Agric Sci 55(1):11–33
Dobson HE, Arroyo J, Bergström G, Groth I (1997) Interspecific variation in floral fragrances within the genus Narcissus (Amaryllidaceae). Biochem Syst Ecol 25(8):685–706
DuBois M, Gilles KA, Hamilton JK, Pt R, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28(3):350–356
Ellis R, RH E, EH R (1980) Towards a rational basis for testing seed quality. In: Hebblethwaite PD (ed) Seed production. Butterworths, London
Farouk S, Al-Huqail AA (2020) Sodium nitroprusside application regulates antioxidant capacity, improves phytopharmaceutical production and essential oil yield of marjoram herb under drought. Indust Crop Prod 158:113034
Filippou P, Antoniou C, Fotopoulos V (2013) The nitric oxide donor sodium nitroprusside regulates polyamine and proline metabolism in leaves of Medicago truncatula plants. Free Radic Biol Med 56:172–183
Gholami J, Ranjbar R, Bahar M, Choupannejad R, Saed Moucheshi S (2018) Molecular detection and characterization of a stolbur phytoplasma associated with Narcissus tazetta phyllody in Iran. J Phytopathol 166(5):372–377
Gul F, Tahir I (2013) An effective protocol for improving vaselife and postharvest performance of cut Narcissus tazetta flowers. J Saudi Soc Agric Sci 12(1):39–46
Gun S (2020) The effect of herbal oil and citric acid on vase life of cut narcissus (Narcissus tazetta L.) flower. J Postharvest Technol 8(4):18–26
Hajihashemi S (2020) Characterization of exogenous nitric oxide effect on Crocus sativus response to different irrigation regimes. J Plant Growth Regul 40:1510–1520
Hajihashemi S, Noedoost F, Geuns J, Djalovic I, Siddique KH (2018) Effect of cold stress on photosynthetic traits, carbohydrates, morphology, and anatomy in nine cultivars of Stevia rebaudiana. Front Plant Sci 9:1430
Hajihashemi S, Noedoost F, Hedayatzadeh F (2019) Characterization of Brassica napus responses to diluted and undiluted industrial wastewater. Physiol Mol Biol Plant 25(6):1469–1482
Hajihashemi S, Brestic M, Kalaji H, Skalicky M, Noedoost F (2020a) Environmental pollution is reflected in the activity of the photosynthetic apparatus. Photosynthetica 58:529–539
Hajihashemi S, Brestic M, Landi M, Skalicky M (2020b) Resistance of Fritillaria imperialis to freezing stress through gene expression, osmotic adjustment and antioxidants. Sci Rep 10(1):1–13
Hajihashemi S, Skalicky M, Brestic M, Pavla V (2020c) Cross-talk between nitric oxide, hydrogen peroxide and calcium in salt-stressed Chenopodium quinoa Willd. At seed germination stage. Plant Physiol Biochem 154:657–664
Hanks GR (2002) The biology of Narcissus. In: Narcissus and Daffodil. CRC Press, pp 21–38
He Y, Tang R-H, Hao Y, Stevens RD, Cook CW, Ahn SM, Jing L, Yang Z, Chen L, Guo F (2004) Nitric oxide represses the Arabidopsis floral transition. Science 305(5692):1968–1971
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198
Huang H, Ullah F, Zhou D-X, Yi M, Zhao Y (2019) Mechanisms of ROS regulation of plant development and stress responses. Front Plant Sci 10:800
Khurana A, Khurana JP, Babbar SB (2011) Nitric oxide induces flowering in the duckweed Lemna aequinoctialis Welw. (Syn. L. paucicostata Hegelm.) under noninductive conditions. J Plant Growth Regul 30(3):378–385
Li X-F, Shao X-H, Deng X-J, Wang Y, Zhang X-P, Jia L-Y, Xu J, Zhang D-M, Sun Y, Xu L (2012) Necessity of high temperature for the dormancy release of Narcissus tazetta var. chinensis. J Plant Physiol 169(14):1340–1347
Naing AH, Lee K, Arun M, Lim KB, Kim CK (2017) Characterization of the role of sodium nitroprusside (SNP) involved in long vase life of different carnation cultivars. BMC Plant Biol 17(1):1–12
Persson S, Wei H, Milne J, Page GP, Somerville CR (2005) Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets. Proc Nat Acad Sci 102(24):8633–8638
Rashed M (2010) Monitoring of contaminated toxic and heavy metals, from mine tailings through age accumulation, in soil and some wild plants at Southeast Egypt. J Hazard Mater 178(1–3):739–746
Ruíz-Ramón F, Águila DJ, Egea-Cortines M, Weiss J (2014) Optimization of fragrance extraction: daytime and flower age affect scent emission in simple and double narcissi. Indust Crop Prod 52:671–678
Savage JA, Haines DF, Holbrook NM (2015) The making of giant pumpkins: how selective breeding changed the phloem of Cucurbita maxima from source to sink. Plant Cell Environ 38(8):1543–1554
Seligman K, Saviani E, Oliveira H, Pinto-Maglio C, Salgado I (2008) Floral transition and nitric oxide emission during flower development in Arabidopsis thaliana is affected in nitrate reductase-deficient plants. Plant Cell Physiol 49(7):1112–1121
Singh A, Banerjee A, Roychoudhury A (2021) Differential responses of Vigna radiata and Vigna mungo to fluoride-induced oxidative stress and amelioration via exogenous application of sodium nitroprusside. J Plant Growth Regul 40:2342–2357
Singleton V, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticul 16(3):144–158
Slezák KA, Mazur J, Jezdinský A, Kapczyńska A (2020) Bulb size interacts with lifting term in determining the quality of Narcissus poeticus l. propagation material. Agronomy 10(7):975
Szôllôsi R, Varga IS (2002) Total antioxidant power in some species of Labiatae (adaptation of FRAP method). Acta Biol Szeged 46(3–4):125–127
Tsuda T, Shiga K, Ohshima K, Kawakishi S, Osawa T (1996) Inhibition of lipid peroxidation and the active oxygen radical scavenging effect of anthocyanin pigments isolated from Phaseolus vulgaris L. Biochem Pharmacol 52(7):1033–1039
Tsujimoto Y, Hashizume H, Yamazaki M (1993) Superoxide radical scavenging activity of phenolic compounds. Int J Biochem 25(4):491–494
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151(1):59–66
Wada KC, Takeno K (2010) Stress-Induced Flowering. Plant Signal Behav 5(8):944–947
Wagner GJ (1979) Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiol 64(1):88–93
Wang Y, Yu L, Nan Z, Liu Y (2004) Vigor tests used to rank seed lot quality and predict field emergence in four forage species. Crop Sci 44(2):535–541
Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144(3):307–313
Zhang X, Zhao X, Wang Z, Shen W, Xu X (2015) Protective effects of hydrogen-rich water on the photosynthetic apparatus of maize seedlings (Zea mays L.) as a result of an increase in antioxidant enzyme activities under high light stress. Plant Growth Regul 77(1):43–56
Zhang ZW, Luo S, Zhang GC, Feng LY, Zheng C, Zhou YH, Du JB, Yuan M, Chen YE, Wang CQ (2017) Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ 40(9):1834–1848
Zhang ZW, Fu YF, Zhou YH, Wang CQ, Lan T, Chen GD, Zeng J, Chen YE, Yuan M, Yuan S (2019) Nitrogen and nitric oxide regulate Arabidopsis flowering differently. Plant Sci 284:177–184
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SH designed the experiment, SH and OJ conducted the experiment, SH and OJ analyzed the data, SH wrote the manuscript, SH proofread the manuscript.
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Hajihashemi, S., Jahantigh, O. Nitric Oxide Effect on Growth, Physiological and Biochemical Processes, Flowering, and Postharvest Performance of Narcissus tazzeta. J Plant Growth Regul 42, 892–907 (2023). https://doi.org/10.1007/s00344-022-10596-3
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DOI: https://doi.org/10.1007/s00344-022-10596-3