Abstract
Species of the red algal order Acrochaetiales mostly inhabit marine environments, with only two freshwater taxa Audouinella and Ottia. A new genus and species are described for freshwater red alga Pauciramus yunnanensis from Ailao Mountain, Yunnan, China. It is closely related to Ottia and a new order Ottiales was proposed for these genera. Pauciramus has unique combination of morphological characteristics including the following: plants caespitose and densely pulvinate, slender uniseriate filaments with well-developed rhizoids, rarely branched, cylindrical vegetative cell with a single, ribbon-shaped and parietal chloroplast, reproduction by tetrasporangia, and dense sporangial branchlet only at the upper portion of filaments. Phylogenetic analysis of sequence data from the plastid ribulose-1,5-bisphosphate carboxylase-oxygenase large-subunit (rbcL), small subunit gene of the ribosomal cistron (SSU) and barcode region near the 5′ end of the mitochondrial cytochrome oxidase subunit I (COI-5P) indicated that: the new taxon, P. yunnanensis, was in a well-supported clade with Ottia meiospora, and this clade was sister to order Palmariales and Acrochaetiales. To adhere to the principle of monophyly, a new freshwater order Ottiales including Ottia and Pauciramus is proposed. Despite the high sequence interspecific divergences and obvious morphological differences between genera Ottia and Pauciramus, seems impractical to establish a new family for a monospecific genus. Therefore, we temporarily classified Pauciramus into the family Ottiaceae, and made necessary revisions to the description to accommodate this genus.
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Data Availability Statement
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Chen L, Feng J, Han X J, Xie S L. 2014. Investigation of a freshwater acrochaetioid alga (Rhodophyta) with molecular and morphological methods. Nordic Journal of Botany, 32(5): 529–535, https://doi.org/10.1111/njb.00407.
Chiasson W B, Johanson K G, Sherwood A R, Vis M L. 2007. Phylogenetic affinities of form taxon Chantransia pygmaea (Rhodophyta) specimens from the Hawaiian Islands. Phycologia, 46(3): 257–262.
Chiasson W B, Sabo N J, Vis M L. 2005. Affinities of freshwater putative chantransia stages (Rhodophyta) from molecular and morphological data. Phycologia, 44(2): 163–168, https://doi.org/10.2216/0031-8884(2005)44[163:AOFPCS]2.0.CO;2.
Choi H G, Kraft G T, Saunders G W. 2000. Nuclear small-subunit rDNA sequences from Ballia spp. (Rhodophyta): proposal of the Balliales ord. nov., Balliaceae fam. nov., Ballia nana sp. nov. and Inkyuleea gen. nov. (Ceramiales). Phycologia, 39(4): 272–287, https://doi.org/10.2216/i0031-8884-39-4-272.1.
Clayden S L, Saunders G W. 2008. Resurrecting the red algal genus Grania within the order Acrochaetiales (Florideophyceae, Rhodophyta). European Journal of Phycology, 43(2): 151–160, https://doi.org/10.1080/09670260801896630.
Clayden S L, Saunders G W. 2014. A study of two Acrochaetium complexes in Canada with distinction of Rhododrewia gen. nov. (Acrochaetiales, Rhodophyta). Phycologia, 53(3): 221–232, https://doi.org/10.2216/13-224.1.
Entwisle T J, Evans J R, Vis M L, Saunders G W. 2018. Ottia meiospora (Ottiaceae, Rhodophyta), a new genus and family endophytic within the thallus of Nothocladus (Batrachospermales, Rhodophyta). Journal of Phycology, 54(1): 79–84, https://doi.org/10.1111/jpy.12603.
Freshwater, D W, Rueness, J. 1994. Phylogenetic relationships of some European Gelidium (Gelidiales, Rhodophyta) species, based on rbcL nucleotide sequence analysis. Phycologia, 33(3): 187–194, https://doi.org/10.2216/i0031-8884-33-3-187.1
Garbary D J. 1987. The Acrochaetiaceae (Rhodophyta): an annotated bibliography. Bibliotheca Phycologica, 77: 1–267.
Guindon S, Dufayard J F, Lefort V, Anisimova M, Hordijk W, Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59(3): 307–321, https://doi.org/10.1093/sysbio/syq010.
Guiry M D, Guiry G M. 2021. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. https://www.algaebase.org. Accessed on Apr. 25, 2021.
Hall T A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95–98.
Harper J T, Saunders G W. 2001. The application of sequences of the ribosomal cistron to the systematics and classification of the Florideophyte red algae (Florideophyceae, Rhodophyta). Cahiers De Biologie Marine, 42: 25–38.
Harper J T, Saunders G W. 2002. A re-classification of the Acrochaetiales based on molecular and morphological data, and establishment of the Colaconematales ord. nov. (Florideophyceae, Rhodophyta). European Journal of Phycology, 37(3): 463–476, https://doi.org/10.1017/s0967026202003840.
Harper J, Saunders G. 1998. A molecular systematic investigation of the Acrochaetiales (Florideophycidae, Rhodophyta) and related taxa based on nuclear small-subunit ribosomal DNA sequence data. European Journal of Phycology, 33(3): 221–229, https://doi.org/10.1080/09670269810001736723.
Jao C C. 1940. Studies on the freshwater algae of China. IV. Subaerial and aquatic algae from Nanyoh, Hunan. Sinensia, 11: 241–360.
Jao C C. 1941. Studies on the freshwater algae of China. VIII. A preliminary account of the Chinese freshwater Rhodophyceae. Sinensia, 12: 245–289.
Kalyaanamoorthy S, Minh B Q, Wong T K F, von Haeseler A, Jermiin L S. 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods, 14(6): 587–589, https://doi.org/10.1038/nmeth.4285.
Kumano S. 2002. Freshwater Red Algae of the World. Biopress Ltd, Bristol, UK. 375p.
Lam D W, Verbruggen H, Saunders G W, Vis M L. 2016. Multigene phylogeny of the red algal subclass Nemaliophycidae. Molecular Phylogenetics & Evolution, 94: 730–736, https://doi.org/10.1016/j.ympev.2015.10.015.
Lanfear R, Frandsen P B, Wright A M, Senfeld T, Calcott B. 2016. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution, 34(3): 772–773, https://doi.org/10.1093/molbev/msw260.
Müller K M, Sherwood A R, Pueschel C M, Gutell R R, Sheath R G. 2002. A proposal for a new red algal order, the Thoreales. Journal of Phycology, 38(4): 807–820, https://doi.org/10.1046/j.1529-8817.2002.01055.x.
Necchi O Jr, Oliveira M C. 2011. Phylogenetic affinities of “chantransia” stages in members of the Batrachospermales and Thoreales (Rhodophyta). Journal of Phycology, 47(3): 680–686, https://doi.org/10.1111/j.1529-8817.2011.00997.x.
Necchi O, Zucchi M R. 1997. Audouinella macrospora (Acrochaetiaceae, Rhodophyta) is the Chantransia stage of Batrachospermum (Batrachospermaceae). Phycologia, 36(3): 220–224. https://doi.org/10.2216/i0031-8884-36-3-220.1.
Nguyen L T, Schmidt H A, von Haeseler A, Minh B Q. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology & Evolution, 32(1): 268–274, https://doi.org/10.1093/molbev/msu300.
Pueschel C M, Saunders G W, West J A. 2000. Affinities of the freshwater red alga Audouinella macrospora (Florideophyceae, Rhodophyta) and related forms based on SSU rRNA gene sequence analysis and pit plug ultrastructure. Journal of Phycology, 36(2): 433–440, https://doi.org/10.1046/j.1529-8817.2000.99173.x.
Ronquist F, Teslenko M, Van Der Mark P, Ayres D L, Darling A, Höhna S, Larget B, Liu L, Suchard M A, Huelsenbeck J P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61(3): 539–542, https://doi.org/10.1093/sysbio/sys029.
Saunders G W, Bird C J, Ragan MA, Rice E L. 1995. Phylogenetic relationships of species of uncertain taxonomic position within the Acrochaetiales-Palmariales complex (Rhodophyta): inferences from phenotypic and 18s rDNA sequence data. Journal of Phycology, 31(4): 601–611, https://doi.org/10.1111/j.1529-8817.1995.tb02556.x.
Saunders G W, Jackson C, Salomaki E D. 2018. Phylogenetic analyses of transcriptome data resolve familial assignments for genera of the red-algal Acrochaetiales-Palmariales Complex (Nemaliophycidae). Molecular Phylogenetics and Evolution, 119: 151–159, https://doi.org/10.1016/j.ympev.2017.11.002.
Saunders G W. 1993. Gel purification of red algal genomic DNA: an inexpensive and rapid method for the isolation of polymerase chain reaction-friendly DNA. Journal of Phycology, 29(2): 251–254, https://doi.org/10.1111/j.0022-3646.1993.00251.x.
Saunders G W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philosophical transactions of the Royal Society B: Biological Sciences, 360(1462): 1879–1888, https://doi.org/10.1098/rstb.2005.1719.
Scott F J, Saunders G W, Kraft G T. 2013. Entwisleia bella, gen. et sp. nov., a novel marine “batrachospermaceous” red alga from southeastern Tasmania representing a new family and order in the Nemaliophycidae. European Journal of Phycology, 48(4): 398–410, https://doi.org/10.1080/09670262.2013.849359.
Sheath R G, Müller K M. 1999. Systematic status and phylogenetic relationships of the freshwater genus Balbiania (Rhodophyta). Journal of Phycology, 35(4): 855–864. https://doi.org/10.1046/j.1529-8817.1999.3540855.x.
Sheath R G, Vis M L. 2015. Red algae. In: Wehr J D, Sheath R G, Kociolek J eds. Freshwater algae of North America. 2nd ed. Academic Press, San Diego, California. p.237–264, https://doi.org/10.1016/B978-0-12-385876-4.00005-0.
Shi Z X. 2006. Flora algarum sinicarum aquae dulcis, Tomus XIII, Rhodophyta, Phaeophyta. Science Press, Beijing. 208p. (in Chinese)
Skinner S, Entwisle T J. 2001. Non-marine algae of Australia: 3. Audouinella and Balbiania (Rhodophyta). Telopea, 9(3): 713–723, https://doi.org/10.7751/telopea20024009.
Skuja H. 1944. Untersuchungen über die Rhodophyceen des Süsswassers (VII-XII). Acta Horti Botanici Universitatis Latviensis, 14: 3–64.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731–2739, https://doi.org/10.1093/molbev/msr121.
Thiers B. 2021. (Constantly updated). Index Herbariorum: A Global Directory of Public Herbaria and Associated Staff. New York Botanical Garden’s Virtual Herbarium, New York. https://sweetgum.nybg.org/ih/. Accessed on Apr. 25, 2021.
Vis M L, Saunders G W, Sheath R G, Dunse K, Entwisle T J. 1998. Phylogeny of the Batrachospermales (Rhodophyta) inferred from rbcL and 18S ribosomal DNA gene sequences. Journal of Phycology, 34(2): 341–350, https://doi.org/10.1046/j.1529-8817.1998.340341.x.
Vis M L, Sheath R G. 1997. Biogeography of Batrachospermum gelatinosum (Batrachospermales, Rhodophyta) in North America based on molecular and morphological data. Journal of Phycology, 33(3): 520–526, https://doi.org/10.1111/j.0022-3646.1997.00520.x.
Volovsek M E, Hommersand M H, Manos P. 2000. The phylogenetics of Acrochaetiales revisited: inferences from rbcL and chloroplast encoded 23s rRNA sequence analysis. Journal of Phycology, 36(S3): 68–69, https://doi.org/10.1046/j.1529-8817.1999.00001-203.x.
Xie S L, Shi Z X. 2004. Balbiania Sirodot — a new record of freshwater red algae from China. Acta Botanica Boreali-Occidentalia Sinica, 24(9): 1732–1733, https://doi.org/10.3321/j.issn:1000-4025.2004.09.030. (in Chinese with English abstract)
Zhang D, Gao F L, Jakovlić I, Zou H, Zhang J, Li W X, Wang G T. 2020. PhyloSuite: an integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources, 20(1): 348–355, https://doi.org/10.1111/1755-0998.13096.
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Supported by the National Natural Science Foundation of China (No. 41871037 to Shulian XIE and No. 31800172 to Fangru NAN) and the Fund for Shanxi “1331 Project”
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Pauciramus yunnanensis, gen. et sp. nov., a novel freshwater red alga from China with proposal of the Ottiales ord. nov. (Nemaliophycidae, Rhodophyta)
343_2021_1154_MOESM3_ESM.doc
Pauciramus yunnanensis, gen. et sp. nov., a novel freshwater red alga from China with proposal of the Ottiales ord. nov. (Nemaliophycidae, Rhodophyta)
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Fang, K., Nan, F., Feng, J. et al. Pauciramus yunnanensis, gen. et sp. nov., a novel freshwater red alga from China with proposal of the Ottiales ord. nov. (Nemaliophycidae, Rhodophyta). J. Ocean. Limnol. 40, 1245–1256 (2022). https://doi.org/10.1007/s00343-021-1154-2
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DOI: https://doi.org/10.1007/s00343-021-1154-2