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Molecular characterization and expression of the SiUCP2 gene in sea urchin Strongylocentrotus intermedius

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Abstract

Uncoupling protein 2 (UCP2) is a proton transporter located in the inner mitochondrial membrane, and inhibits the formation of adenosine triphosphate and reactive oxygen species by uncoupling oxidative phosphorylation. To provide a theoretical basis for the role of SiUCP2 in lipid metabolism, a 2 341-bp full-length cDNA of SiUCP2 from sea urchin Strongylocentrotus intermedius, which encodes 323 amino acids (predicted MW 36.11 kDa) was obtained, and the structure and function of the SiUCP2 gene and its expression at the mRNA and protein level were studied. SiUCP2 had high homology with UCP2 of other species. Expression of SiUCP2 was detected in the order of tube feet > gonads > coelomocytes > intestines. The expression level was the highest in prismatic larvae and lowest in the two-cell stage. Moreover, using in-situ hybridization, we found that SiUCP2 protein was expressed in the gonads and intestine. This study provided a theoretical basis for subsequent studies on the role of SiUCP2 and its regulatory mechanism in lipid metabolism, and for the improvement of gonad quality to obtain a higher economic value from sea urchins.

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Data Availability Statement

The data used to support the findings of this study are available from the corresponding author upon request.

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Authors and Affiliations

  1. Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China

    Lingshu Han, Zijiao Quan, Bing Han, Beichen Ding, Xiaofang Huang, Heng Wang, Yaqing Chang & Jun Ding

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  1. Lingshu Han
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  2. Zijiao Quan
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  3. Bing Han
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  5. Xiaofang Huang
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  8. Jun Ding
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Contributions

Jun DING, Bing HAN, and Lingshu HAN contributed to the conception of the study. Lingshu HAN and Zijiao QUAN performed the experiments. Lingshu HAN, Zijiao QUAN, Xiaofang HUANG, and Beichen DING performed the data analyses. Lingshu HAN drafted the manuscript. Lingshu HAN, Jun DING, Bing HAN, and Heng WANG revised the manuscript. Jun DING and Yaqing CHANG approved the final version.

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Correspondence to Jun Ding.

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Supported by the National Key Research and Development Program of China (No. 2018YFD0901601) and Chinese Outstanding Talents in Agricultural Sciences (for Yaqing CHANG).

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Han, L., Quan, Z., Han, B. et al. Molecular characterization and expression of the SiUCP2 gene in sea urchin Strongylocentrotus intermedius. J. Ocean. Limnol. 39, 1523–1537 (2021). https://doi.org/10.1007/s00343-020-0181-8

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